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Efficient short-read sequencing on long-read sequencers
2020We present SMURF-seq, a protocol to efficiently sequence short DNA molecules on a long-read sequencer by randomly ligating them to form long molecules. Applying SMURF-seq using the highly portable and inexpensive Oxford Nanopore MinION yields up to 30 fragments per read, providing an average of 6.2 and up to 7.5 million mappable fragments per run ...
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Long-read Sequencing Technology
This 50 minute lecture on long-read sequencing technology covers the following items: 1) Review of RNA-Seq Short-read Sequencing, 2) Overview and benefits of Long-read Sequencing, 3) Oxford Nanopore Sequencing, and 4) Pacific Biosciences Sequencing.openaire +1 more source
Genome sequencing: Long reads for a short plant
Nature Plants, 2015The genome of a tiny resurrection plant has been sequenced using PacBio's long-read single-molecule real-time sequencing technology, aiding the understanding of extreme desiccation tolerance. The genome contiguity is comparable to that of genomes sequenced using far more laborious approaches.
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Unveiling microbial diversity: harnessing long-read sequencing technology
Nature MethodsLong-read sequencing has recently transformed metagenomics, enhancing strain-level pathogen characterization, enabling accurate and complete metagenome-assembled genomes, and improving microbiome taxonomic classification and profiling. These advancements are not only due to improvements in sequencing accuracy, but also happening across rapidly changing
Daniel P. Agustinho +5 more
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Transcriptomics in the era of long-read sequencing
Nature Reviews GeneticsTranscriptome sequencing revolutionized the analysis of gene expression, providing an unbiased approach to gene detection and quantification that enabled the discovery of novel isoforms, alternative splicing events and fusion transcripts. However, although short-read sequencing technologies have surpassed the limited dynamic range of previous ...
Carolina Monzó +2 more
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Ottelia alismoides long-read RNA sequenceing
This project sequenced Ottelia alismoides leaves RNA sequences under high and low CO2 treatments at dark and light conditions. Total RNA from two different treatments at two different times were mixed together for sequencing. There long-read sequences were used to provide gene sequences.openaire +1 more source
Long‐Read Sequencing Could Increase Diagnosis Rates
American Journal of Medical Genetics Part A, 2021openaire +2 more sources
Treatment of muscle‐invasive and advanced bladder cancer in 2020
Ca-A Cancer Journal for Clinicians, 2020Vaibhav G Patel +2 more
exaly
Iso-Seq Long Read Transcriptome Sequencing
2021Nam V. Hoang, Robert J. Henry
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