Results 151 to 160 of about 2,208 (185)

Optimizing the performance of digital loop-mediated isothermal amplification

Analytical Biochemistry, 2021
Digital loop-mediated isothermal amplification (dLAMP) is attractive for the detection of nucleic acid due to its superior characteristics including isothermal amplification, absolute quantification, and single-molecule sensitivity. However, dLAMP suffers from the inaccurate quantification caused by low digital efficiency, which means only part of ...
Yingying, Xue, Xinyi, Luo, Xueyuan, Pang, Jianhua, Zhou, Jiasi, Wang   +4 more
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Recombinase assisted loop-mediated isothermal DNA amplification

The Analyst, 2020
A novel method named RALA which combines the advantages of recombinase and loop-mediated amplification was developed. Fast and sequence-specific detection was achieved by introducing a probe named ProofMan.
Gangyi Chen, Rong Chen, Sheng Ding, Mei Li, Jiayu Wang, Jiawei Zou, Feng Du, Juan Dong, Xin Cui, Xin Huang, Yun Deng, Zhuo Tang   +11 more
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Detection of porcine parvovirus by loop-mediated isothermal amplification

Journal of Virological Methods, 2009
Loop-mediated isothermal amplification is a novel method for rapid amplification of DNA. It has been adopted widely for the detection of virus because of its simplicity, rapidity, and specificity. A loop-mediated isothermal amplification assay was developed for the detection of porcine parvovirus.
Chang-mu, Chen, Shang-jin, Cui
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Detection of fish nocardiosis by loop-mediated isothermal amplification

Journal of Applied Microbiology, 2006
Loop-mediated isothermal amplification (LAMP) is a novel method that amplifies DNA with high specificity and rapidity under isothermal conditions. In this study, using the LAMP method, a protocol for detecting Nocardia seriolae which is a causative agent of fish nocardiosis, was designed.A set of four primers, two inner and two outer, were designed ...
T, Itano, H, Kawakami, T, Kono, M, Sakai
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Loop-Mediated Isothermal Amplification for the Detection of Plant Pathogens

2012
Loop-mediated isothermal amplification (LAMP) is a technique involving the use of four to six primers (two inner primers, two outer primers, and two loop primers) and the strand displacement activity of Bacillus subtilis-derived (Bst) DNA polymerase.
Lisa I, Ward, Scott J, Harper
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Loop‐Mediated Isothermal Amplification for Detection of Nucleic Acids

Current Protocols in Molecular Biology, 2014
AbstractSequence‐specific isothermal nucleic acid amplification techniques are ideally suited for use in molecular diagnostic applications because they do not require thermal cycling equipment and the reactions are typically fast. One of the most widely cited isothermal techniques is termed loop‐mediated isothermal amplification (LAMP).
Nathan A, Tanner, Thomas C, Evans
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[Loop-mediated isothermal amplification].

Nihon rinsho. Japanese journal of clinical medicine, 2007
We have developed the loop-mediated isothermal amplification (LAMP) method, an innovative gene amplification technique, which has the potential to become important in genetic testing. This method allows highly efficient amplification by a single enzyme at a constant temperature. It also has features such as high specificity, rapidity, and simplicity of
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Colorimetric strategies applicable for loop-mediated isothermal amplification

Journal of Microbiological Methods
In recent years, loop-mediated isothermal amplification (LAMP) has gained popularity for detecting various pathogen-specific genes due to its superior sensitivity and specificity compared to conventional polymerase chain reaction (PCR). The simplicity and flexibility of naked-eye detection of the amplicon make LAMP an ideal rapid and straightforward ...
Syaidatul Akmal, Saifuddin, Roslina, Rashid, Nurin Jazlina, Nor Azmi, Suharni, Mohamad   +3 more
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A loop-mediated isothermal amplification assay for the diagnosis of pulmonary tuberculosis

Letters in Applied Microbiology, 2019
Quantitated Mycobacterium tuberculosis (M.tb) H37Rv DNA was used to analyse the sensitivity and the specificity was assessed using DNA isolated from the reference strain H37Rv, 12 nontuberculous mycobacterium (NTM) species and five nonmycobacterium species.
G. Sharma, R. Tewari, S.K. Dhatwalia, R. Yadav, D. Behera, S. Sethi   +5 more
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In-Field Diagnostics Using Loop-Mediated Isothermal Amplification

2012
Loop-mediated isothermal amplification (LAMP) is a method for amplification and detection of target organisms which, unlike polymerase chain reaction, does not require thermal cycling. LAMP assays can be developed in the laboratory for subsequent deployment in the field, where the simplicity of isothermal amplification makes LAMP a suitable method for ...
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