Results 131 to 140 of about 48,371 (318)

Smartphone-based pathogen diagnosis in urinary sepsis patients. [PDF]

, 2018
BackgroundThere is an urgent need for rapid, sensitive, and affordable diagnostics for microbial infections at the point-of-care. Although a number of innovative systems have been reported that transform mobile phones into potential diagnostic tools, the
Barnes, Lucien, Choe, Jane, Fitzgibbons, Lynn N, Fox, Gary N, Fried, Jeffrey C, Heithoff, Douglas M, Mahan, Michael J, Mahan, Scott P, Marth, Jamey D, Soh, H Tom, Zambrano, Andrea   +10 more
core  

Thermostable Bioluminescent Intercalating Dyes for Real‐Time, Integrated Nucleic Acid Amplification and Detection

Angewandte Chemie, EarlyView.
We present a bioluminescent diagnostic platform that integrates DNA amplification and detection in a single step. The engineering of thermostable luciferase‐intercalating dye conjugates and controlled release of caged luciferin substrates enable real‐time, detection of attomolar concentrations of viral DNA within 30 min.
Yosta de Stigter, Harmen J. van der Veer, Sterre de Lignie, Robbert J. de Haas, Renko de Vries, Joost P. H. Schoeber, Anne J. M. Loonen, Adriaan J. C. van den Brule, Maarten Merkx   +8 more
wiley   +2 more sources

Loop mediated isothermal amplification (LAMP) of Theileria annulata DNA [PDF]

, 2017
In the past three decades, as an alternative to PCR (polymerase chain reaction) new diagnostic techniques like LAMP (loop mediated isothermal amplification) whereby target DNA can be amplified under isothermal conditions without using thermocycler have
Bakirci, Serkan, Bilgiç, Hüseyin Bilgin, Eren, Hasan, Karagenç, Tülin, Weir, William   +4 more
core   +1 more source

SDS‐CRISPR for Single‐Nucleotide Variant Detection

Advanced Science, EarlyView.
Structure‐disruption‐sensitive CRISPR (SDS‐CRISPR) converts structural instability into single‐nucleotide precision, thereby overcoming mismatch tolerance in canonical Cas12a and enabling versatile diagnostics across DNA, RNA, and microRNA targets. When applied to rapid IDH1 mutation detection for glioma genotyping and integrated with lateral‐flow ...
Xin Guan, Chong Guo, Jiongyu Zhang, Rui Yang, Yerramsetti Ramachandra, Chengyu Hou, Minjie Pei, Shuo Zhang, Kurt T. Schalper, Xingye Liu, Qian Wu, Ketan R. Bulsara, Changchun Liu   +12 more
wiley   +1 more source

A simple, high-throughput, colourimetric, field applicable loop-mediated isothermal amplification (HtLAMP) assay for malaria elimination. [PDF]

, 2015
BACKGROUND: To detect all malaria infections in elimination settings sensitive, high throughput and field deployable diagnostic tools are required. Loop-mediated isothermal amplification (LAMP) represents a possible field-applicable molecular diagnostic ...
AF Vallejo, B Dinko, CJ Sutherland, CM Scheel, Colin J. Sutherland, CV Plowe, D Padley, E Han, F Lu, G Kiddle, G Snounou, H Hopkins, H Iseki, J Cook, James S. McCarthy, JC Patel, L Luo, LC Okell, LC Okell, LL Poon, M Goto, M Sema, M Yamamura, MJ Grigg, MS Hsiang, N Tomita, NW Lucchi, PS Walsh, Qin Cheng, RE Coleman, S Dittrich, S Yongkiettrakul, SD Polley, SD Polley, SL Wastling, Sumudu Britton, T Bousema, U Morris, WHO, WHO, WHO, X Ou, Y Arimatsu, Y Mori, YL Lau, ZK Njiru   +45 more
core   +3 more sources

Multidimensional and Multifunctional Laser‐Induced Graphene (LIG) for Point‐of‐Care and Wearable Biosensing, Theranostics, and Bioactive Interfaces Toward Personalized Healthcare and Regenerative Medicine

Advanced Science, EarlyView.
Multidimensional laser‐induced graphene (LIG) spanning from 0D to 3D architectures is comprehensively reviewed for multifunctional biomedical platforms, including biosensing, theranostics, and bioactive interface applications, which highlights its potentials for point‐of‐care diagnostics, wearable health monitoring, smart drug delivery, and tissue ...
Li Zhang, Yurou Yuan, Xiaohong Ding, Wing Cheung Mak   +3 more
wiley   +1 more source

Recent trends in molecular diagnostics of yeast infections : from PCR to NGS [PDF]

, 2019
The incidence of opportunistic yeast infections in humans has been increasing over recent years. These infections are difficult to treat and diagnose, in part due to the large number and broad diversity of species that can underlie the infection.
Arastehfar, A, Boekhout, T, Buda De Cesare, G, Butler, G, Dolk, E, Gabaldón, Tony, Hafez, A, Hagen, F, Hovhannisyan, H, Hube, B, Iracane, E, Kostrzewa, M, Lackner, M, Lass-Flörl, C, Llorens, C, Mixão, V, Munro, C, Oliveira-Pacheco, J, OPATHY Consortium, the, Pekmezovic, M, Pérez-Hansen, A, Rodriguez Sánchez, Antonio, Sauer, FM, Sparbier, K, Stavrou, AA, Vaneechoutte, Mario, Vatanshenassan, M   +26 more
core   +2 more sources

Rapid detection of Mycoplasma synoviae by loop-mediated isothermal amplification [PDF]

Archives of Microbiology, 2014
Mycoplasma synoviae (MS) remains a serious concern in production of poultry and affects world production of chickens and turkeys. Loop-mediated isothermal amplification (LAMP) of DNA has been recently used for the identification of different economically important avian pathogens.
Kursa, Olimpia, Woźniakowski, Grzegorz, Tomczyk, Grzegorz, Sawicka, Anna, Minta, Zenon   +4 more
openaire   +2 more sources

DNA Nanoarray for Multiplexed RNA Detection With Single‐Molecule Readout

Advanced Science, EarlyView.
A carrier‐based DNA nanoarray integrates programmable nucleic acid hybridization with solid‐state nanopore readout for direct RNA detection at single‐molecule resolution. A ternary encoding system enables 27 carriers with 81 addressable sensing sites, allowing the simultaneous identification of targets spanning a wide size range, from short microRNAs ...
Yunxuan Li, Yesheng Wang, Chunmiao Yu, Siyu Hou, Elli Mylona, Plamena Naydenova, Jingyuan Zhao, Hong Yuan, Jingsong Duan, Hendrik F. P. Runge, Stephen Baker, Jinbo Zhu, Ulrich F. Keyser   +12 more
wiley   +1 more source

Ultrasensitive loop mediated isothermal amplification (US-LAMP) to detect malaria for elimination

Malaria Journal, 2019
Background Malaria elimination requires diagnostic methods able to detect parasite levels well below what is currently possible with microscopy and rapid diagnostic tests.
Abu Naser Mohon, Sisay Getie, Nusrat Jahan, Mohammad Shafiul Alam, Dylan R. Pillai   +4 more
doaj   +1 more source