Results 121 to 130 of about 2,335,448 (272)

Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for rapid detection of Actinobacillus pleuropneumoniae based the dsbE-like gene

open access: yesPesquisa Veterinária Brasileira, 2012
This paper reports on the development and validation of a loop-mediated isothermal amplification assay (LAMP) for the rapid and specific detection of Actinobacillus pleuropneumoniae (A. pleuropneumoniae).
Hongwei Ji   +7 more
doaj   +1 more source

Revolutionizing Healthcare With Paper‐Based Nucleic Acid Testing

open access: yesExploration, EarlyView.
This work summarizes strategies to enhance paper‐based devices for nucleic acid testing. Key approaches include optimizing paper platforms, improving nucleic acid amplification, and refining labeling/signaling methods. These advancements aim to boost sensitivity, speed, and usability, making paper‐based diagnostics more effective for point‐of‐care ...
Hong Zhang   +8 more
wiley   +1 more source

Reverse transcription loop-mediated isothermal amplification (RT-LAMP) HIV-1 assay specifications.

open access: yes, 2014
Reverse transcription loop-mediated isothermal amplification (RT-LAMP) HIV-1 assay specifications.
Rachel Johns (666475)   +10 more
core   +1 more source

Flexible photocatalytic materials: From substrate engineering to multi‐field synergistic enhancement

open access: yesFlexMat, EarlyView.
This review examines flexible photocatalytic systems, covering substrate evolution from polymers to biomass, fabrication methods like electrospinning, and key mechanical and optoelectrochemical metrics under deformation. Enhancement strategies are critically discussed, followed by applications in energy conversion, environmental remediation, and ...
Weiqi Wang   +3 more
wiley   +1 more source

Loop-Mediated Isothermal Amplification for Diagnosis of Zoonotic Malaria

open access: yesThe American Journal of Tropical Medicine and Hygiene
Zoonotic malaria, caused by Plasmodium knowlesi, Plasmodium cynomolgi, Plasmodium coatneyi, and Plasmodium inui, is a significant global health concern. The gold standard microscopy, while widely used for malaria diagnosis, faces limitations in differentiating between malaria species.
Lai, Meng Yee   +4 more
openaire   +2 more sources

Rapid In-Field Detection of Airborne Pathogens Using Loop-Mediated Isothermal Amplification (LAMP) [PDF]

open access: yes
Multiple human and plant pathogens are dispersed and transmitted as bioaerosols (e.g., Mycobacterium tuberculosis, SARS-CoV-2, Legionella pneumophila, Aspergillus fumigatus, Phytophthora spp., and Fusarium graminearum).
Bani, A.   +14 more
core   +1 more source

Deciphering transcriptome complexity via long‐read sequencing

open access: yesiMeta, EarlyView.
Long‐read sequencing is transforming transcriptomics from gene‐level quantification to isoform‐resolved interpretation by directly resolving full‐length transcript structures, transcription in repetitive regions, and linked molecular features. This review provides an end‐to‐end roadmap covering sequencing platforms, library preparation strategies ...
Chuwen Xu   +21 more
wiley   +1 more source

Enhanced CRISPR‐cas systems for genome editing and molecular diagnostics

open access: yesInterdisciplinary Medicine, EarlyView.
This review systematically summarizes the major optimization strategies for CRISPR‐Cas systems, focusing on the engineering of Cas proteins, guide RNAs, reporter probes, and the incorporation of chemical additives. The aim is to enhance editing precision and diagnostic capability, thereby expanding the CRISPR system's potential for broader biomedical ...
Sheng Li   +7 more
wiley   +1 more source

Loop-Mediated Isothermal Amplification Targeting Actin DNA of Trichomonas vaginalis

open access: yes, 2020
Trichomoniasis, which is caused by the protist, Trichomonas vaginalis, is one of the most common non-viral sexually transmitted infections, with an estimated prevalence of 276 million new infections worldwide each year The most common clinical ...
So-Young Joo   +9 more
core  

Low‐Concentration Enhancement Effect: Enabling Sensitive Detection of EML4‐ALK Variants via Multiplex RT‐MIRA

open access: yesJournal of Clinical Laboratory Analysis, EarlyView.
This study developed an optimized multiplex RT‐MIRA assay for the simultaneous detection of EML4‐ALK variants, achieving single‐copy sensitivity. Unexpectedly, during the development of the reaction system, we discovered a “low‐concentration enhancement effect,” where reducing primer or probe concentrations within a specific range markedly improved ...
Xiao‐Dong Ren   +10 more
wiley   +1 more source

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