Results 121 to 130 of about 48,371 (318)

Loop-Mediated Isothermal Amplification Detection of SARS-CoV-2 and Myriad Other Applications.

Journal of Biomolecular Techniques, 2021
As the second year of the COVID-19 pandemic begins, it remains clear that a massive increase in the ability to test for SARS-CoV-2 infections in a myriad of settings is critical to controlling the pandemic and to preparing for future outbreaks.
Keith J M Moore, J. Cahill, Guy Aidelberg, R. Aronoff, A. Bektaş, D. Bezdan, D. Butler, S. Chittur, Martin Codyre, Fernán Federici, N. Tanner, S. Tighe, R. True, S. B. Ware, A. Wyllie, Evan E. Afshin, Andrés Bendesky, Connie B. Chang, Richard Dela Rosa, E. Elhaik, D. Erickson, A. Goldsborough, G. Grills, Kathrin Hadasch, A. Hayden, Seong-Young Her, J. Karl, Chang Hee Kim, Alison J. Kriegel, T. Kunstman, Zeph Landau, Kevin Land, B. Langhorst, A. Lindner, Benjamin E. Mayer, L. McLaughlin, M. McLaughlin, Jenny Molloy, C. Mozsary, J. Nadler, M. D'Silva, D. Ng, D. O’Connor, J. Ongerth, O. Osuolale, Ana Pinharanda, D. Plenker, Ravikant Ranjan, M. Rosbash, A. Rotem, J. Segarra, S. Schürer, S. Sherrill-Mix, H. Solo-Gabriele, S. To, Merly C. Vogt, Albert D. Yu, C. Mason   +57 more
semanticscholar   +1 more source

Mass‐Produced and High‐Performance Nanowell Biosensor Fabricated via Semiconductor Manufacturing for Rapid and Accurate COVID‐19 Diagnosis in the Clinical Field

Advanced Science, EarlyView.
A semiconductor‐fabricated nanowell biosensor enables rapid, scalable, and highly reproducible detection of SARS‐CoV‐2 antigens from nasal swabs within ∼10 minutes. Clinical validation in 249 retrospective and 243 prospective patient samples demonstrates high sensitivity and specificity, minimal cross‐reactivity, and robust batch‐to‐batch ...
Yoo Min Park, Zahra Rezaei, Nam Ho Bae, Donggee Rho, Da‐Seul Kim, EunYoung Go, YoungTae Seo, Seok Jae Lee, Won Chan Seo, Luke P. Lee, HeaYeon Lee, Su Ryon Shin   +11 more
wiley   +1 more source

A Modular and Programmable Cas13d Platform for RNA Single Nucleotide Variant Detection

Advanced Science, EarlyView.
A scenario‐guided Cas13d platform enables configurable single nucleotide variant ribonucleic acid diagnostics through rule‐based guide design and ribonucleic acid binding domain engineering, supporting amplification‐free ultrasensitive detection and amplification‐coupled robust detection for accurate mutation classification in clinical tumor specimens.
Zeyu Wang, Jiahao Li, Zhuying Yue, Beixuan He, Wenhua Zhang, Zhen Fang, Qing Xia, Yingbin Liu, Yanjing Li   +8 more
wiley   +1 more source

Assessing the presence of Wuchereria bancrofti in vector and human populations from urban communities in Conakry, Guinea [PDF]

, 2015
The Global Programme to Eliminate Lymphatic Filariasis was launched in 2000 with the goal of interrupting transmission of lymphatic filariasis (LF) through multiple rounds of mass drug administration (MDA).
Boakye, Daniel A., Bockarie, Moses J., Dadzie, Samuel K., de Souza, Dziedzom K., Diakité, Lamia, Goepogui, Andre, King, Sandra A., Kouassi, Bernard L., Koudou, Benjamin G., Mamadou, Baldé S., Narh, Charles A., Utzinger, Jürg   +11 more
core   +4 more sources

Toward Multi(radio)metalated DNA: Enzymatic Polymerization of Metal‐Chelate‐Modified Deoxyribonucleoside Triphosphates

Angewandte Chemie, EarlyView.
This work presents the first enzymatic polymerization of defined metal‐chelator‐modified nucleotides that leverage polymerase fidelity for uniform (radio)metal loading with successful incorporation of five different metals: Ga, In, Tb, Lu, and Y.
Antonio A. W. L. Wong, François Bénard, David M. Perrin   +2 more
wiley   +2 more sources

A portable device for nucleic acid quantification powered by sunlight, a flame or electricity. [PDF]

, 2018
A decentralized approach to diagnostics can decrease the time to treatment of infectious diseases in resource-limited settings. Yet most modern diagnostic tools require stable electricity and are not portable.
Cesarman, Ethel, Duru, Jens, Erickson, David, Gardner, Andrea, Kopparthy, Varun Lingaiah, Martin, Jeffrey, Maurer, Toby, Semeere, Aggrey, Snodgrass, Ryan   +8 more
core   +1 more source

Detektion des Kartoffelspindelknollen Viroids mit Hilfe der Loop Mediated Isothermal Amplification [PDF]

, 2011
PSTV ist ein hoch infektiöses Viroid, das in Kartoffeln verkleinerte und spindelähnliche Knollen verursacht. Um Ernteverlusten vorzubeugen, ist eine Detektion in frühen Infektionsstadien von großer Bedeutung.
Frohme, Marcus, Glökler, Jörn, Kadolsky, Marianne, Schulze, Katja   +3 more
core   +1 more source

TopCas: Topology‐Gated Cas12a via DNA‐RNA Chimeric Circular crRNA for Amplification‐Free Nucleic Acid Detection and Conditional Gene Editing

Advanced Science, EarlyView.
The TopCas platform utilizes an engineered DNA‐RNA chimeric circular crRNA to establish topology‐gated control over CRISPR‐Cas12a activity, enabling target‐triggered self‐catalytic amplification (preamplification‐free). This system seamlessly integrates highly sensitive molecular diagnostics with conditionally controlled gene editing, demonstrating ...
Shun Zhang, Wen Sun, Ting Xiao, Yali Wang, Xianlan Wu, Huiyou Chen, Ming Chen, Jun Zhang   +7 more
wiley   +1 more source

A Portable Colorimetric Device for Rapid Bacterial Detection with Cleavable Functional Nucleic Acid Probes for A Common Bacterial Endoribonuclease

Angewandte Chemie, EarlyView.
A simple, rapid, and portable gold‐coated filter tip‐based assay (GFTA) is developed for bacterial detection. The GFTA leverages cleavable functional nucleic acid probes as molecular recognition elements to target RNase H2, a highly conserved bacterial endoribonuclease, enabling sensitive and specific colorimetric detection of bacterial pathogens ...
Jiuxing Li, Rudi Liu, Wenqing Zhang, Bruno J. Salena, Yingfu Li   +4 more
wiley   +2 more sources

A Novel CsYABBY3‐CsAS1 Feedback Loop Coordinates Trichome Differentiation and Cannabinoid Biosynthesis in Cannabis sativa L.

Advanced Science, EarlyView.
A novel transcriptional module involving CsYABBY3 and CsAS1 is identified to regulate cannabinoid biosynthesis and trichome development. These factors mutually activate each other and form a protein complex via a conserved residue, acting synergistically to amplify metabolic flux through a coordinate feed‐forward mechanism.
Xuewen Zhu, Yaolei Mi, Xue Cao, Weiqiang Chen, Pucheng Fan, Jing Wang, Yiming Zhang, Wei Yang, Huihua Wan, Shanshan Chen, Xiangxiao Meng, Jun Li, Shuo Shen, Mingkun Huang, Xiaoyu Zhang, Ming Luo, Shilin Chen, Zhichao Xu, Wei Sun   +18 more
wiley   +1 more source