Results 101 to 110 of about 48,371 (318)

Loop mediated isothermal amplification of Clostridioides difficile isolates in gastrointestinal patients

AMB Express, 2022
This study investigated the prevalence of Clostridioides difficile by culture, multiplex polymerase chain reaction (M-PCR), and loop mediated isothermal amplification (LAMP) in patients with suspected C. difficile infections (CDIs).
Mojtaba Moosavian, Razieyeh Keshavarzi, Effat Abbasi Montazeri, Eskandar Hajiani   +3 more
doaj   +1 more source

Paper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis C virus

Nature Communications, 2021
The early diagnosis of active hepatitis C virus (HCV) infection remains a significant barrier to the treatment of the disease and to preventing the associated significant morbidity and mortality seen, worldwide. Current testing is delayed due to the high
Weronika Witkowska McConnell, Chris Davis, S. Sabir, Alice Garrett, A. Bradley-Stewart, Pawel Jajesniak, J. Reboud, Gaolian Xu, Zhugen Yang, R. Gunson, E. Thomson, J. Cooper   +11 more
semanticscholar   +1 more source

A Bifunctional T3SS‐Effector Simultaneously Cleaves Host MAP Kinase and Inhibits PPM1A Phosphatase

Advanced Science, EarlyView.
Pathogenic bacteria exploit the metalloprotease effector NleD to subvert host defenses. Structural, biochemical, and infection analyses reveal a bifunctional mechanism by which NleD binds and inhibits the host phosphatase PPM1A while preserving its proteolytic activity against MAPKs.
Yaakov Socol, Lihi Gur‐Arie, Netanel Tzarum, Tamara Wellins, Naama Katsowich, Miriam Ravins, Michal Bejerano‐Sagie, Klil Cohen, Oded Livnah, Joshua N. Adkins, Ernesto Nakayasu, Alexei Savchenko, Yeu Khai Choong, Nikhil Kumar Tulsian, Saturo Machida, Sigal Ben‐Yehuda, Yael Litvak, J. Sivaraman, Ilan Rosenshine   +18 more
wiley   +1 more source

Detection of Mycobacterium ulcerans by the loop mediated isothermal amplification method. [PDF]

PLoS Neglected Tropical Diseases, 2012
BACKGROUND: Buruli ulcer (BU) caused by Mycobacterium ulcerans (M. ulcerans) has emerged as an important public health problem in several rural communities in sub-Saharan Africa.
Anthony Ablordey, Diana Ackon Amissah, Isaac Frimpong Aboagye, Ben Hatano, Toshio Yamazaki, Tetsutaro Sata, Koichi Ishikawa, Harutaka Katano   +7 more
doaj   +1 more source

Loop-Mediated Isothermal Amplification (LAMP): A Rapid, Sensitive, Specific, and Cost-Effective Point-of-Care Test for Coronaviruses in the Context of COVID-19 Pandemic

Biology, 2020
The rampant spread of COVID-19 and the worldwide prevalence of infected cases demand a rapid, simple, and cost-effective Point of Care Test (PoCT) for the accurate diagnosis of this pandemic.
R. Augustine, A. Hasan, Suvarthi Das, Rashid Ahmed, Yasuyoshi Mori, T. Notomi, Bhavesh D. Kevadiya, Avnesh S Thakor   +7 more
semanticscholar   +1 more source

Zinc Exposure Causes Disulfidptosis to Induce Miscarriage by Up‐Regulating GATA1/METTL1/SLC7A11 Axis

Advanced Science, EarlyView.
Zn exposure up‐regulates GATA1, promoting GATA1‐mediated METTL1 and SLC7A11 transcription. It also enhances METTL1‐mediated m7G modification on SLC7A11 mRNA, increasing SLC7A11 mRNA stability. Ultimately, Zn exposure up‐regulates SLC7A11 at both transcriptional and post‐transcriptional levels, causing disulfidptosis. Knockdown of murine Slc7a11, Gata1,
Wenxin Huang, Yi Sun, Yanxin Wang, Haijun Yan, Xiaoping Yue, Weidong Wu, Xueyu Chen, Jinfang Zhang, Yanbing Lin, Qiong Lei, Nan Ji, Shuaishuai Xing, Liqin Zeng, Qingzheng Kang, Depeng Zhao, Geng Guo, Huidong Zhang   +16 more
wiley   +1 more source

Loop-Mediated Isothermal Amplification as a Simple Molecular Method for the Detection of Derzsy’s Disease Virus

Journal of Veterinary Research, 2013
The objective of the study was to develop a simple and rapid molecular method for the detection of GPV. Twenty seven goose parvovirus (GPV) isolates collected from geese flocks in Poland were examined.
Tarasiuk Karolina, Woźniakowski Grzegorz, Samorek-Salamonowicz Elżbieta   +2 more
doaj   +1 more source

Real-time fluorescence loop mediated isothermal amplification for the diagnosis of malaria. [PDF]

PLoS ONE, 2010
BACKGROUND: Molecular diagnostic methods can complement existing tools to improve the diagnosis of malaria. However, they require good laboratory infrastructure thereby restricting their use to reference laboratories and research studies.
Naomi W Lucchi, Allison Demas, Jothikumar Narayanan, Deborah Sumari, Abdunoor Kabanywanyi, S Patrick Kachur, John W Barnwell, Venkatachalam Udhayakumar   +7 more
doaj   +1 more source

Development of a recombinase polymerase amplification lateral flow assay for the detection of active Trypanosoma evansi infections [PDF]

, 2020
Author summary Neglected tropical diseases (NTDs) affecting humans and/or domestic animals severely impair the socio-economic development of endemic areas. One of these diseases, animal trypanosomosis, affects livestock and is caused by the parasites of
Goossens, Julie, Li, Zeng, Magez, Stefan, Pinto Torres, Joar Esteban, Sterckx, Yann G-J, Stijlemans, Benoit   +5 more
core   +1 more source

All-in-One Digital Microfluidics System for Molecular Diagnosis with Loop-Mediated Isothermal Amplification

Biosensors, 2022
In this study, an “all-in-one” digital microfluidics (DMF) system was developed for automatic and rapid molecular diagnosis and integrated with magnetic bead-based nucleic acid extraction, loop-mediated isothermal amplification (LAMP), and real-time ...
Siyi Hu, Yuhan Jie, K. Jin, Yifan Zhang, Tianjie Guo, Qi Huang, Q. Mei, Fuqiang Ma, Hanbin Ma   +8 more
semanticscholar   +1 more source