Results 101 to 110 of about 21,508 (262)

Ultra-sensitive detection of Plasmodium falciparum by amplification of multi-copy subtelomeric targets [PDF]

, 2015
Even when applying standard molecular diagnostics, prevalence in our study population was underestimated by 8% compared to the new assays. Our findings highlight the need for highly sensitive tools such as TARE-2 and varATS qPCR in community surveillance
Ingrid Felger (92160), Robinson, LJ, Shekalaghe, Seif, Natalie Hofmann (697415), Seif Shekalaghe, Felger, Ingrid, Mwingira, F, Ingrid Felger, Ivo Mueller (46609), Natalie Hofmann, Hofmann, Natalie, Mwingira, Felista, Felger, I, Leanne J. Robinson (137914), Hofmann, N, Mueller, Ivo, Felista Mwingira, Seif Shekalaghe (79961), Ivo Mueller, Hofmann, Natalie E., Mueller, I, Felista Mwingira (697416), Shekalaghe, S, Leanne J Robinson, Robinson, Leanne J.   +24 more
core   +1 more source

A Novel miRNA Detection Method Using Loop-Mediated Isothermal Amplification

, 2023
A novel ligation-based loop-mediated isothermal amplification has been developed for miRNA detection. Two stem-loop structure DNA linker A/B probes which hybridized with miRNA were designed to establish a rapid and ultrasensitive miRNA-LAMP system for ...
Qun Li, Jiatong Liu, Sheng Cai, Saiwei Wu, Abdu Ahmed Abdullah Al-Maskri   +4 more
core   +1 more source

Development of a loop‐mediated isothermal amplification‐lateral flow dipstick (LAMP‐LFD) assay and on‐site rapid detection evaluation for Rodentibacter heylii and Rodentibacter pneumotropicus

Animal Models and Experimental Medicine, EarlyView.
This study established a closed‐tube LAMP‐LFD assay for detecting Rodentibacter heylii and Rodentibacter pneumotropicus. The method exhibited high sensitivity (detection limits of 10−5 ng/μL for R. heylii and 10−4 ng/μL for R. pneumotropicus), high specificity, reliable performance with clinical samples, and effective validation in experimental ...
Huiqiong Yan, Sisi Chen, Yuhan Gan, Jing Xing, Yufang Feng, Xin Wen, Jie Fang, Jiangtao Du, Shasang Zhou, Honggang Guo, Xiaoyin Jin, Zhiyuan Wang, Junhao Tao, Lingqun Lu, Qingming Kong, Huazhong Ying, Wei Han, Fangwei Dai   +17 more
wiley   +1 more source

Isothermal nucleic acid amplification techniques: A comprehensive overview on their principle & applications as alternative to PCR

Practical Laboratory Medicine
Isothermal nucleic acid amplification methods have gained prominence as a valuable innovation in molecular diagnostics. These methods function at a constant temperature without the requirement of thermal cycler apparatus, simplifying the amplification ...
Alazar Amare Amdiyee, Tesfaye Sisay Tessema   +1 more
doaj   +1 more source

Designing loop-mediated isothermal amplification (LAMP) and assimilating probes

, 2020
On March 28, 2020, IPANEMA organized the first Scientific online lecture (SOL1) for the project’s partners. dr Ryo Kubota, a molecular biologist from Diagenetix - a member of the IPANEMA consortium, was presenting his scientific expertise in designing ...
Ryo Kubota
core   +1 more source

Rapid In-Field Detection of Airborne Pathogens Using Loop-Mediated Isothermal Amplification (LAMP) [PDF]

Multiple human and plant pathogens are dispersed and transmitted as bioaerosols (e.g., Mycobacterium tuberculosis, SARS-CoV-2, Legionella pneumophila, Aspergillus fumigatus, Phytophthora spp., and Fusarium graminearum).
Bani, A., Ian Colbeck, Alessia Bani, Dumbrell, Alex J, Robert M. W. Ferguson, Colbeck, I., Corinne Whitby, Ferguson, Robert MW, Alex J. Dumbrell, Bani, Alessia, Whitby, Corinne, Colbeck, Ian, Ferguson, R. M. W., Whitby, C., Dumbrell, A. J.   +14 more
core   +1 more source

Detection of Pseudomonas fluorescens from broth, water and infected tissues by loop-mediated isothermal amplification (LAMP) method

, 2015
Loop mediated isothermal amplification is rapid, highly sensitive and specifically developed method for detection of bacterial infections. AprX gene for alkaline metalloprotease of Pseudomonas fluorescens was used to design four primers and loop mediated
Saharan, P, Gahlawat, SK, Duhan, JS
core   +1 more source

Emerging Technologies for the Detection of Rabies Virus: Challenges and Hopes in the 21st Century [PDF]

, 2009
The diagnosis of rabies is routinely based on clinical and epidemiological information, especially when exposures are reported in rabies-endemic countries.
Müller, Thomas, Banyard, AC, Conrad M Freuling, Freuling, Conrad Martin, Dastjerdi, A., McElhinney, LM, Dastjerdi, A, Akbar Dastjerdi, Anthony R Fooks, Weiss, RA, Thomas Müller, Freuling, C.M., McElhinney, L.M., Edward Wright, Banyard, A.C., Lorraine M McElhinney, Fooks, AR, Johnson, N., Wright, E, Muller, T, Fooks, A.R., Wakeley, P.R., Wakeley, PR, Robin A Weiss, Philip R Wakeley, Johnson, N, Weiss, R.A., Marston, D.A., Muller, T., Marston, DA, Denise A Marston, Ashley C Banyard, Wright, E., Nicholas Johnson, Freuling, CM   +34 more
core   +1 more source

CO2‐Driven Synthesis of Holey Edge‐Carboxylated Graphene for Tunable Electronic Interactions in Cu–CeO2‐Catalyzed Water‐Gas Shift Reaction

Carbon Energy, EarlyView.
Holey‐structured, edge‐carboxylated graphene with a controlled specific surface area was synthesized using an environmentally friendly, CO2‐activated, KOH‐free ball‐milling process. ABSTRACT Graphene, notable for its high specific surface area, is ideal for catalyst support but faces challenges under harsh thermal catalytic conditions.
Seon‐Yong Ahn, I‐Jeong Jeon, Won‐Jun Jang, In‐Yup Jeon, Jae‐Oh Shim, Hyun‐Seog Roh   +5 more
wiley   +1 more source

Isothermal loop-mediated amplification (LAMP) for diagnosis of contagious bovine pleuro-pneumonia. [PDF]

, 2013
BACKGROUND Contagious Bovine Pleuropneumonia (CBPP) is the most important chronic pulmonary disease of cattle on the African continent causing severe economic losses. The disease, caused by infection with Mycoplasma mycoides subsp.
Joachim Frey, Wade, Abel, Hermann Unger, Vilei, Edy, Abel Wade, Frey, Joachim, Georg Mair, Edy M Vilei, Mair, Georg, Unger, Hermann   +9 more
core   +1 more source