Results 91 to 100 of about 48,371 (318)

Kinetics of elementary steps in loop-mediated isothermal amplification (LAMP) show that strand invasion during initiation is rate-limiting

Nucleic Acids Research, 2022
Loop-mediated isothermal amplification (LAMP) has proven to be easier to implement than PCR for point-of-care diagnostic tests. However, the underlying mechanism of LAMP is complicated and the kinetics of the major steps in LAMP have not been fully ...
Tyler L. Dangerfield, Inyup Paik, S. Bhadra, K. Johnson, A. Ellington   +4 more
semanticscholar   +1 more source

Bacteria‐Responsive Nanostructured Drug Delivery Systems for Targeted Antimicrobial Therapy

Advanced Materials, EarlyView.
Bacteria‐responsive nanocarriers are designed to release antimicrobials only in the presence of infection‐specific cues. This selective activation ensures drug release precisely at the site of infection, avoiding premature or indiscriminate release, and enhancing efficacy.
Guillermo Landa, Gracia Mendoza, Silvia Irusta, Manuel Arruebo   +3 more
wiley   +1 more source

Development of SARS-CoV-2 Isothermal Amplification Detection Kits

Nano Biomedicine and Engineering, 2020
The SARS-CoV-2 isothermal amplification detection kits based on loop-mediated isothermal amplification (LAMP) were developed and evaluated on three types samples of SARS-CoV-2. The kits included enzyme reaction mixtures and chromogenic agents.
Xueling Li, Jing Tian, Yan Xu, Xiaomin Chen, Zexi Liu, Hui Liang, Caihong Jin, Daxiang Cui   +7 more
doaj   +1 more source

Development of a LAMP assay for detection of Leishmania infantum infection in dogs using conjunctival swab samples [PDF]

, 2015
Background: Leishmania infantum infections in dogs play a crucial role in the transmission of pathogens causing visceral leishmaniasis to humans in the Gansu province, northwest China. To be able to control zoonotic transmission of the parasite to humans,
A Moshfe, A Nasereddin, AF Koutinas, CH Gao, Chun-hua Gao, D Otranto, D Strauss-Ayali, Dan Ding, Dietmar Steverding, ER Adams, F Dantas-Torres, Feng Shi, G Schönian, H Kaneko, H Takagi, J Alvar, J Dereure, JM Lawrie, Jun-yun Wang, JY Wang, JY Wang, JY Wang, K Stuart, L Chamaillé, L He, L Solano-Gallego, LL Poon, LS Leontides, M Chaouch, M Gramiccia, M Paiva Cavalcanti de, MG Khan, N Chargui, N Kuboki, N Singh, P Ciaramella, RS Leite, S Reale, SA Ferreira, T Muccio Di, T Notomi, UG Lopes, WP Kennedy, X Ni, Xia Wang, Y Fichoux le, Y Mori, YM Gomes, Yue-tao Yang   +48 more
core   +1 more source

Droplet Encoding‐Pairing Enabled Multiplexed Digital Loop‐Mediated Isothermal Amplification for Simultaneous Quantitative Detection of Multiple Pathogens

Advancement of science, 2023
Despite the advantages of digital nucleic acid analysis (DNAA) in terms of sensitivity, precision, and resolution, current DNAA methods commonly suffer a limitation in multiplexing capacity.
Dongyang Cai, Yu Wang, Jingjing Zou, Zhujun Li, Enqi Huang, Xiuyun Ouyang, Z. Que, Yanzhang Luo, Zhenhua Chen, Yanqing Jiang, Guohao Zhang, Hongkai Wu, Dayu Liu   +12 more
semanticscholar   +1 more source

Sea Urchin‐Inspired Immuno‐Instructive Ionic Flow Drives MSCs‐Macrophage Communication to Promote Bone Regeneration

Advanced Materials, EarlyView.
ABSTRACT Bone tissue regeneration is a complex physiological process dependent on the spatiotemporal coordination of immune cells and stem cells. Conventional research primarily elucidates the mechanism through which materials facilitate bone formation by initially modulating macrophages and subsequently encouraging the osteogenic differentiation of ...
Yang Lu, Kun Su, Daqian Liu, Chunyu Liu, Muyan Qin, Shuaijie Li, Pengfei Tian, Yansong Wang, Yingbo Wang, Lei Chu, Xu Cui, Haobo Pan   +11 more
wiley   +1 more source

Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits [PDF]

, 2020
The success of fundamental and applied nucleic acid (NA) research depends on NA purity, but obtaining pure NAs from raw, unprocessed samples is challenging.
Ismagilov, Rustem F., Jue, Erik, Witters, Daan   +2 more
core  

Fluorometric Paper-Based, Loop-Mediated Isothermal Amplification Devices for Quantitative Point-of-Care Detection of Methicillin-Resistant Staphylococcus aureus (MRSA).

ACS Sensors, 2021
Loop-mediated isothermal amplification (LAMP) has been widely used to detect many infectious diseases. However, minor inconveniences during the steps of adding reaction ingredients and lack of simple color results hinder point-of-care detection.
I. Choopara, Akkapol Suea-Ngam, Y. Teethaisong, P. Howes, M. Schmelcher, A. Leelahavanichkul, S. Thunyaharn, D. Wongsawaeng, A. deMello, D. Dean, N. Somboonna   +10 more
semanticscholar   +1 more source

Detection of Human Influenza A Viruses by Loop-Mediated Isothermal Amplification [PDF]

Journal of Clinical Microbiology, 2005
ABSTRACT Here we describe the use of the loop-mediated isothermal amplification (LAMP) method to detect human influenza viruses (H1 to H3). Our results were correlated 100% with results deduced from routine clinical diagnostic tests. In addition, we also developed a LAMP assay specific for human β-actin cDNA as a quality control test.
Chan, KH, Lee, JHC, Leung, CSW, Poon, LLM, Yuen, KY, Peiris, JSM, Guan, Y   +6 more
openaire   +4 more sources

Advances in Electroactive Liquid Crystal Elastomers for Intelligent Robotics and Electronics

Advanced Robotics Research, EarlyView.
Electroactive liquid crystal elastomers (eLCEs) integrate molecular anisotropy with electrical functionality to enable programmable actuation and reconfigurable electronics. This review classifies eLCEs into robotic actuators and adaptive electronics, summarizes key actuation mechanisms, and highlights fabrication strategies that bridge materials to ...
Kavita Ramesh Rathod, Sena Chon, Jae Hyuk Hwang, Sung Hyuk Park, Woohwa Lee, Joonmyung Choi, Shi Hyeong Kim, Seung Hwan Ko, Changsoon Choi, Seon Jeong Kim, Dong‐Gyun Kim, Habeom Lee, Kyung Gook Cho, Woon‐Hong Yeo, Hyun Kim   +14 more
wiley   +1 more source