Results 71 to 80 of about 48,371 (318)

Molecular Methods for Identification and Quantification of Foodborne Pathogens

open access: yesMolecules, 2022
Foodborne pathogens that enter the human food chain are a significant threat worldwide to human health. Timely and cost-effective detection of them became challenging for many countries that want to improve their detection and control of foodborne ...
Min Zhang   +7 more
doaj   +1 more source

The burden of congenital Chagas disease and implementation of molecular diagnostic tools in Latin America [PDF]

open access: yes, 2018
It is estimated that between 8000 and 15 000 Trypanosoma cruzi infected babies are born every year to infected mothers in Chagas disease endemic countries. Currently, poor access to and performance of the current diagnostic algorithm, based on microscopy
Cruz, Israel   +7 more
core   +1 more source

Point‐of‐Care Diagnostic Platforms for Loop‐Mediated Isothermal Amplification

open access: yesAdvanced Engineering Materials, 2022
The loop‐mediated isothermal amplification (LAMP) method is one of the Nucleic acid amplification tests (NAATs) that allows for the amplification of target regions without using a thermal cycle.
Nazente Atçeken   +4 more
semanticscholar   +1 more source

Diagnosis of Ruminant Viral Diseases with Loop-Mediated Isothermal Amplification

open access: yesMolecular Biotechnology, 2023
Infectious diseases in livestock industry are major problems for animal health, food safety, and the economy. Zoonotic diseases from farm animals are significant threat to human population as well. These are notifiable diseases listed by the World Organization for Animal Health (OIE).
Sanem Ayaz Kök   +2 more
openaire   +3 more sources

Biosensors Based on Isothermal DNA Amplification for Bacterial Detection in Food Safety and Environmental Monitoring

open access: yesSensors, 2021
The easy and rapid spread of bacterial contamination and the risk it poses to human health makes evident the need for analytical methods alternative to conventional time-consuming laboratory-based techniques for bacterial detection. To tackle this demand,
Sandra Leonardo   +2 more
doaj   +1 more source

Detection of foot-and-mouth disease virus rna by reverse transcription loop-mediated isothermal amplification [PDF]

open access: yes, 2011
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for foot-and-mouth disease virus (FMDV) RNA. The amplification was able to finish in 45 min under isothermal condition at 64°C by employing a set of four primers
Hao-tai Chen   +3 more
core   +1 more source

Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR Technology

open access: yesAnalytical Chemistry, 2020
We have developed a single-tube assay for SARS-CoV-2 in patient samples. This assay combined advantages of reverse transcription (RT) loop-mediated isothermal amplification (LAMP) with clustered regularly interspaced short palindromic repeats (CRISPRs ...
B. Pang   +15 more
semanticscholar   +1 more source

Inhibition of Non-specific Amplification in Loop-Mediated Isothermal Amplification via Tetramethylammonium Chloride

open access: yesBioChip Journal, 2022
Loop-mediated isothermal amplification (LAMP) may be used in molecular and point-of-care diagnostics for pathogen detection. The amplification occurs under isothermal conditions using up to six primers.
Minju Jang, Sanghyo Kim
semanticscholar   +1 more source

A novel cassette method for probe evaluation in the designed biochips [PDF]

open access: yes, 2014
A critical step in biochip design is the selection of probes with identical hybridisation characteristics. In this article we describe a novel method for evaluating DNA hybridisation probes, allowing the fine-tuning of biochips, that uses cassettes with ...
Al-humam, Abdulmohsen A.   +7 more
core   +4 more sources

Rapid Detection of Novel Coronavirus (COVID-19) by Reverse Transcription-Loop-Mediated Isothermal Amplification

open access: yesmedRxiv, 2020
Novel Corona virus (COVID-19 or 2019-nCoV) is an emerging global health concern that requires a rapid diagnostic test. Quantitative reverse transcription PCR (qRT-PCR) is currently the standard for COVID-19 detection; however, Reverse Transcription Loop ...
Laura E. Lamb   +3 more
semanticscholar   +1 more source

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