Results 51 to 60 of about 48,371 (318)
Nucleic acid extraction-free isothermal amplification based on magnetic beads capture and ligation [PDF]
Jichu yixue yu linchuang, 2021 Objective Capture and ligation probe-loop-mediated isothermal amplification CLIP-LAMP method with-out nucleic acid extraction based on magnetic beads was established to provide a new technique for the detection of human immunodeficiency virus (HIV) in
QIU Li-zhen, YANG Ming-zhu, PIAN Hong-ru, ZHENG Zhi
doaj
Journal of Molecular Diagnostics, 2020 The coronavirus disease 2019 (COVID-19) pandemic now has >2,000,000 confirmed cases worldwide. COVID-19 is currently diagnosed using quantitative RT-PCR methods, but the capacity of quantitative RT-PCR methods is limited by their requirement of high ...
Gun-Soo Park +6 more
semanticscholar +1 more source
Loop-Mediated Isothermal Amplification for Detection of African Trypanosomes [PDF]
Journal of Clinical Microbiology, 2003 ABSTRACTWhile PCR is a method of choice for the detection of African trypanosomes in both humans and animals, the expense of this method negates its use as a diagnostic method for the detection of endemic trypanosomiasis in African countries. The loop-mediated isothermal amplification (LAMP) reaction is a method that amplifies DNA with high specificity,
Noritaka, Kuboki +7 more
openaire +2 more sources
Capture and ligation-based loop-mediated isothermal amplification technology [PDF]
Jichu yixue yu linchuang, 2020 Objective To establish a capture and ligation-based loop-mediated isothermal amplification technology (CLIP-LAMP) based on probe capture and ligation reaction to provide a new method for laboratory diagnosis and pathogen detection.
PIAN Hong-ru, YANG Ming-zhu, ZHENG Zhi
doaj
Viruses, 2022 A recent outbreak of monkeypox virus (mpox) has prompted researchers to explore diagnostics as a means of impeding transmission and further spread. Rapid, sensitive, and specific methods are crucial for accurately diagnosing mpox infections.
Chao Yu +8 more
doaj +1 more source
BioTechniques, 2020 Loop-mediated isothermal amplification (LAMP) is a versatile technique for detection of target DNA and RNA, enabling rapid molecular diagnostic assays with minimal equipment.
Yinhua Zhang +5 more
semanticscholar +1 more source
Low-Temperature Loop-Mediated Isothermal Amplification Operating at Physiological Temperature
Biosensors, 2023 Loop-mediated isothermal amplification (LAMP) is one of the most widely used isothermal amplification technologies in molecular diagnostics. However, LAMP operates at a high temperature of 65 °C; thus, operating LAMP at a lower temperature is desirable ...
Daehan Nam +9 more
semanticscholar +1 more source
PLoS ONE, 2020 A number of isothermal DNA amplification technologies claim to be ideal for point-of-need (PON) applications as they enable reactions to be performed using a single-temperature heat source (e.g. water bath).
Yiping Zou +2 more
doaj +1 more source
Clinical Chemistry, 2020 Background Rapid, reliable, and widespread testing is required to curtail the ongoing COVID-19 pandemic. Current gold standard nucleic acid tests are hampered by supply shortages in critical reagents including nasal swabs, RNA extraction kits, personal ...
M. Lalli +12 more
semanticscholar +1 more source
Current Research in Microbial Sciences, 2022 Highlights • Significance of LAMP method in rapid disease diagnosis is highlighted.• Different detection methods for amplicon visualization are explained.• Advancements in LAMP technique for disease identification are summarized.• Trends in development ...
Nupur Garg, F. Ahmad, S. Kar
semanticscholar +1 more source