Results 41 to 50 of about 21,508 (262)

Determining putative vectors of the Bogia Coconut Syndrome Phytoplasma using loop-mediated isothermal amplification of single insect-feeding media [PDF]

, 2016
Phytoplasmas are insect vectored mollicutes responsible for disease in many economically important crops. Determining which insect species are vectors of a given phytoplasma is important for managing disease but is methodologically challenging because ...
Lu, Hengyu, Ash, Gavin J., Fletcher, Murray J., You, Minsheng, Yang, Guang, Gurr, Geoff M., Woruba, Sharon B., Wilson, Bree A. L.   +7 more
core   +1 more source

Evaluation and improvement of isothermal amplification methods for point-of-need plant disease diagnostics.

PLoS ONE, 2020
A number of isothermal DNA amplification technologies claim to be ideal for point-of-need (PON) applications as they enable reactions to be performed using a single-temperature heat source (e.g. water bath).
Yiping Zou, Michael Glenn Mason, Jose Ramon Botella   +2 more
doaj   +1 more source

Asymmetric stem-loop–mediated isothermal amplification of nucleic acids for DNA diagnostic assays by simple modification of canonical PCR primers

Frontiers in Bioengineering and Biotechnology, 2022
Nucleic acid–based assays have been adopted as mainstream tools for clinical diagnostics, food safety, and environment monitoring with the merits of accuracy, rapidity, and sensitivity.
Rui Mao, Rui Mao, Xinyao Wu, Xinyao Wu, Qing Miao, Qing Miao, Ting Cai, Ting Cai   +7 more
doaj   +1 more source

Loop‐mediated isothermal amplification of single pollen grains [PDF]

Journal of Integrative Plant Biology, 2014
AbstractThe polymerase chain reaction (PCR) has been a reliable and fruitful method for many applications in ecology. Nevertheless, unavoidable technical and instrumental requirements of PCR have limited its widespread application in field situations. The recent development of isothermal DNA amplification methods provides an alternative to PCR, which ...
Ali, Bektaş, Ignacio, Chapela
openaire   +2 more sources

Accelerated loop-mediated isothermal amplification method for the rapid detection of Streptococcus suis serotypes 2 and 14 based on single nucleotide polymorphisms

Frontiers in Cellular and Infection Microbiology, 2022
Streptococcus suis serotypes 2 and 14 are the most prevalent zoonotic strains. The establishment of a sensitive and extremely accurate method for point-of-care testing for Streptococcus suis serotype 2 and 14 strains is highly desirable. In this study, a
Jiajia Meng, Chunling Li, Chunling Li, Chunling Li, Chunling Li, Yu Wang, Zhibiao Bian, Zhibiao Bian, Zhibiao Bian, Zhibiao Bian, Pinpin Chu, Pinpin Chu, Pinpin Chu, Pinpin Chu, Shaolun Zhai, Shaolun Zhai, Shaolun Zhai, Shaolun Zhai, Dongxia Yang, Dongxia Yang, Dongxia Yang, Dongxia Yang, Shuai Song, Shuai Song, Shuai Song, Shuai Song, Yan Li, Yan Li, Yan Li, Yan Li, Zhiyong Jiang, Zhiyong Jiang, Zhiyong Jiang, Zhiyong Jiang, Kunli Zhang, Kunli Zhang, Kunli Zhang, Kunli Zhang, Yugu Li, Hongchao Gou, Hongchao Gou, Hongchao Gou, Hongchao Gou   +42 more
doaj   +1 more source

A Field Deployable Real-Time Loop-Mediated Isothermal Amplification Targeting Five Copy nrdB Gene for the Detection of ‘Candidatus Liberibacter asiaticus’ in Citrus [PDF]

The Plant Pathology Journal, 2023
Huanglongbing (HLB) is one of the most destructive diseases in citrus, which imperils the sustainability of citriculture worldwide. The presumed causal agent of HLB, ‘Candidatus Liberibacter asiaticus’ (CLas) is a non-culturable phloem-limited α ...
Tirumalareddy Danda, Jong-Won Park, Kimberly L. Timmons, Mamoudou Sétamou, Eliezer S. Louzada, Madhurababu Kunta   +5 more
doaj   +1 more source

Simultaneous detection of multiple benzimidazole resistant β-tubulin variants of Botrytis cinerea using loop mediated isothermal amplification [PDF]

, 2018
Optimal disease management depends on the ability to monitor the development of fungicide resistance in plant pathogen populations. Benzimidazole resistance is caused by the point mutations of the β-tubulin gene in Botrytis cinerea, and three mutations (
Gero Steinberg, Ya Bing Duan, Chang Jun Chen, Zhou, M. G., Jian Xin Wang, Yang, Y., Steinberg, G., Chen, C. J., Fraaije, B. A., Duan, Y., Ming Guo Zhou, Wang, J. X., Ying Yang, Bart A Fraaije   +13 more
core   +1 more source

Loop-Mediated Isothermal Amplification in a Core-Shell Bead Assay for the Detection of Tyrosine Kinase AXL Overexpression

, 2021
The upregulated expression of tyrosine kinase AXL has been reported in several hematologic and solid human tumors, including gastric, breast, colorectal, prostate and ovarian cancers.
Nguyen, Nam-Trung, Pradip Singha, Umer, Muhammad, Muhammad Umer, Nhat-Khuong Nguyen, Chin Hong Ooi, Sreejith, Kamalalayam Rajan, Nam-Trung Nguyen, Kasetsirikul, Surasak, Surasak Kasetsirikul, Nguyen, Nhat-Khuong, Shiddiky, Muhammad JA, Muhammad J. A. Shiddiky, Kamalalayam Rajan Sreejith, Singha, Pradip, Ooi, Chin Hong   +15 more
core   +1 more source

Loop-mediated isothermal amplification for the detection of goose circovirus [PDF]

Virology Journal, 2012
Abstract Background Goose circovirus (GCV) presents an immunosuppressive problem in production of geese. The infection’s clinical symptoms include growth retardation or feathering disorders but the infection process may remain non-symptomatic what makes the infected birds more susceptible for secondary viral ...
Woźniakowski Grzegorz, Kozdruń Wojciech, Samorek-Salamonowicz Elżbieta   +2 more
openaire   +3 more sources

Evaluation of loop-mediated isothermal amplification for detecting COVID-19

Journal of Clinical Virology Plus, 2022
The emergence of SARS-CoV-2 has caused worldwide pandemic of COVID-19. Infection is difficult to diagnose early as some patients remain asymptomatic and may carry this virus to other people. Currently, qRT-PCR is the widely accepted mode for detection.
Astuti Giantini, Muhammad Suhaeri, Ardiana Kusumaningrum, Dimas Seto Prasetyo, Windy Sahar, Rakhmad Hidayat, Muhammad Arza Putra, null Hermawan   +7 more
openaire   +3 more sources