Results 21 to 30 of about 2,208 (185)

Loop-mediated isothermal amplification for the rapid detection of Salmonella [PDF]

open access: yesFEMS Microbiology Letters, 2005
Loop-mediated isothermal amplification (LAMP) assay detected Salmonella within 60 min. The 220 strains of 39 serotypes of Salmonella subsp. enterica and 7 strains of Salmonella enterica subsp. arizonae were amplified, but not 62 strains of 23 bacterial species other than Salmonella.
Yukiko, Hara-Kudo   +3 more
openaire   +2 more sources

Probe-free label system for rapid detection of Cronobacter genus in powdered infant formula

open access: yesAMB Express, 2018
Cronobacter species previously known as Enterobacter sakazakii poses high risks to neonates and infants. In this work a rapid detection method was developed which combined loop-mediated isothermal amplification with lateral flow assay for detection of ...
Shiqian Fu   +6 more
doaj   +1 more source

Electric LAMP: Virtual Loop-Mediated Isothermal AMPlification [PDF]

open access: yesISRN Bioinformatics, 2012
We present eLAMP, a PERL script, with Tk graphical interface, that electronically simulates Loop-mediated AMPlification (LAMP) allowing users to efficiently test putative LAMP primers on a set of target sequences. eLAMP can match primers to templates using either exact (via builtin PERL regular expressions) or approximate matching (via the tre-agrep ...
Salinas, Nelson R., Little, Damon P.
openaire   +2 more sources

A Review of Isothermal Amplification Methods and Food-Origin Inhibitors against Detecting Food-Borne Pathogens

open access: yesFoods, 2022
The isothermal amplification method, a molecular-based diagnostic technology, such as loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA), is widely used as an alternative to the time-consuming and labor-intensive
Ye-Ji Moon, So-Young Lee, Se-Wook Oh
doaj   +1 more source

Loop-Mediated Isothermal Amplification: From Theory to Practice

open access: yesRussian Journal of Bioorganic Chemistry, 2022
Increasing the accuracy of pathogen identification and reducing the duration of analysis remain relevant for modern molecular diagnostics up to this day. In laboratory and clinical practice, detection of pathogens mostly relies on methods of nucleic acid amplification, among which the polymerase chain reaction (PCR) is considered the "gold standard ...
Shirshikov, F. V., Bespyatykh, J. A.
openaire   +2 more sources

Detecting amplicons of loop‐mediated isothermal amplification [PDF]

open access: yesMicrobiology and Immunology, 2019
AbstractLoop‐mediated isothermal amplification (LAMP) assays are used to detect diverse pathogens. Initially, LAMP amplicons were detected using electrophoresis; later, real‐time monitoring based on turbidity was developed to overcome the problem of contamination with environmental DNA.
openaire   +2 more sources

Head-to-head comparison of two loop-mediated isothermal amplification (LAMP) kits for diagnosis of malaria in a non-endemic setting

open access: yesMalaria Journal, 2023
Background Light microscopy and rapid diagnostic tests (RDT) have long been the recommended diagnostic methods for malaria. However, in recent years, loop-mediated isothermal amplification (LAMP) techniques have been shown to offer superior performance ...
Anna-Clara Ivarsson   +5 more
doaj   +1 more source

DNA Extraction with DNAzol and LAMP, Performed in a Heating Block as a Simple Procedure for Detection of Mycobacterium tuberculosis in Sputum Specimens

open access: yesMethods and Protocols, 2018
Tuberculosis (TB) remains as a major public health issue in developing countries. Accurate detection is essential for the proper management of patients with active disease.
Álvaro Rodríguez-García   +5 more
doaj   +1 more source

Nucleic acid extraction-free isothermal amplification based on magnetic beads capture and ligation [PDF]

open access: yesJichu yixue yu linchuang, 2021
Objective Capture and ligation probe-loop-mediated isothermal amplification CLIP-LAMP method with-out nucleic acid extraction based on magnetic beads was established to provide a new technique for the detection of human immunodeficiency virus (HIV) in
QIU Li-zhen, YANG Ming-zhu, PIAN Hong-ru, ZHENG Zhi
doaj  

Development of a Novel Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Monkeypox Virus Infections

open access: yesViruses, 2022
A recent outbreak of monkeypox virus (mpox) has prompted researchers to explore diagnostics as a means of impeding transmission and further spread. Rapid, sensitive, and specific methods are crucial for accurately diagnosing mpox infections.
Chao Yu   +8 more
doaj   +1 more source

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