Results 11 to 20 of about 48,371 (318)

Strategies for the Voltammetric Detection of Loop-Mediated Isothermal Amplification

Micromachines, 2023
Loop-mediated isothermal amplification (LAMP) is rapidly developing into an important tool for the point-of-use detection of pathogens for both clinical and environmental samples, largely due to its sensitivity, rapidity, and adaptability to portable ...
Jesse M. Marangoni, Kenneth K. S. Ng, Arezoo Emadi   +2 more
doaj   +3 more sources

Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus

Emerging Infectious Diseases, 2007
We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses.
Shanthi Jayawardena, Chung Y. Cheung, Ian Barr, Kwok H. Chan, Honglin Chen, Yi Guan, J.S. Malik Peiris, Leo L.M. Poon   +7 more
doaj   +5 more sources

Diagnosis of brugian filariasis by loop-mediated isothermal amplification.

PLoS Neglected Tropical Diseases, 2012
In this study we developed and evaluated a Brugia Hha I repeat loop-mediated isothermal amplification (LAMP) assay for the rapid detection of Brugia genomic DNA. Amplification was detected using turbidity or fluorescence as readouts.
Catherine B Poole, Nathan A Tanner, Yinhua Zhang, Thomas C Evans, Clotilde K S Carlow   +4 more
doaj   +3 more sources

Digital Loop-Mediated Isothermal Amplification on a Commercial Membrane [PDF]

ACS Sensors, 2019
In this work, we report digital loop-mediated isothermal amplification (LAMP) or reverse-transcription LAMP (RT-LAMP) on a commercial membrane, without the need for complex chip fabrication or use of specialized equipment.
Hoffmann, Michael R., Huang, Xiao, Lin, Xingyu, Urmann, Katharina, Xie, Xing   +4 more
core   +8 more sources

Loop-mediated isothermal amplification of DNA. [PDF]

Nucleic Acids Research, 2000
We have developed a novel method, termed loop-mediated isothermal amplification (LAMP), that amplifies DNA with high specificity, efficiency and rapidity under isothermal conditions. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA.
T. Notomi, H. Okayama, Harumi Otawara-shi Masubuchi, Toshihiro Yonekawa, Keiko Watanabe, N. Amino, T. Hase   +6 more
semanticscholar   +3 more sources

Evaluation of molecular inhibitors of loop-mediated isothermal amplification (LAMP)

Scientific Reports
Loop-mediated isothermal amplification (LAMP) is a cost-effective and easy-to-perform assay that enables the direct detection of DNA. Its use in point-of-care diagnostic tests is growing, while it has the potential to be used in presumptive on-the-field ...
May Khat Nwe, N. Jangpromma, Lapatrada Taemaitree   +2 more
semanticscholar   +4 more sources

A stoichiometric and pseudo kinetic model of loop mediated isothermal amplification

Computational and Structural Biotechnology Journal, 2020
Loop mediated isothermal amplification (LAMP) is one of the most popular isothermal DNA amplification techniques for research and commercial applications, enabling amplification of both DNA and RNA (with the assistance of reverse transcriptase). The LAMP
Navjot Kaur, Nikhil Thota, Bhushan J. Toley   +2 more
doaj   +3 more sources

Loop-Mediated Isothermal Amplification in Schistosomiasis [PDF]

Journal of Clinical Medicine, 2021
Human schistosomiasis is one of the most important parasitic diseases, causing around 250 million cases (mostly in Africa) and 280,000–500,000 deaths every year. Due to the limited resources and the far-removed nature of many endemic areas, the implementation of new, sensitive and specific diagnostic tools has had little success.
Juan García-Bernalt Diego, Pedro Fernández-Soto, Begoña Febrer-Sendra, Beatriz Crego-Vicente, Antonio Muro   +4 more
openaire   +2 more sources

Advances in Mutation Detection Using Loop-Mediated Isothermal Amplification [PDF]

ACS Omega, 2021
Detection of mutations and single-nucleotide polymorphisms is highly important for diagnostic applications. Loop-mediated isothermal amplification (LAMP) is a powerful technique for the rapid and sensitive detection of nucleic acids. However, LAMP traditionally does not possess the ability to resolve single-nucleotide differences within the target ...
Marcelino Varona, Jared L. Anderson
doaj   +4 more sources

Loop-Mediated Isothermal Amplification (LAMP): The Better Sibling of PCR?

Cells, 2021
In 1998, when the PCR technique was already popular, a Japanese company called Eiken Chemical Co., Ltd. designed a method known as the loop-mediated isothermal amplification of DNA (LAMP).
M. Soroka, Barbara Wasowicz, A. Rymaszewska   +2 more
semanticscholar   +1 more source