Results 31 to 40 of about 2,208 (185)

Capture and ligation-based loop-mediated isothermal amplification technology [PDF]

Jichu yixue yu linchuang, 2020
Objective To establish a capture and ligation-based loop-mediated isothermal amplification technology (CLIP-LAMP) based on probe capture and ligation reaction to provide a new method for laboratory diagnosis and pathogen detection.
PIAN Hong-ru, YANG Ming-zhu, ZHENG Zhi
doaj  

Loop-Mediated Isothermal Amplification for Detection of African Trypanosomes [PDF]

Journal of Clinical Microbiology, 2003
ABSTRACTWhile PCR is a method of choice for the detection of African trypanosomes in both humans and animals, the expense of this method negates its use as a diagnostic method for the detection of endemic trypanosomiasis in African countries. The loop-mediated isothermal amplification (LAMP) reaction is a method that amplifies DNA with high specificity,
Noritaka, Kuboki, Noboru, Inoue, Tatsuya, Sakurai, Francescopaolo, Di Cello, Dennis J, Grab, Hiroshi, Suzuki, Chihiro, Sugimoto, Ikuo, Igarashi   +7 more
openaire   +2 more sources

Asymmetric stem-loop–mediated isothermal amplification of nucleic acids for DNA diagnostic assays by simple modification of canonical PCR primers

Frontiers in Bioengineering and Biotechnology, 2022
Nucleic acid–based assays have been adopted as mainstream tools for clinical diagnostics, food safety, and environment monitoring with the merits of accuracy, rapidity, and sensitivity.
Rui Mao, Rui Mao, Xinyao Wu, Xinyao Wu, Qing Miao, Qing Miao, Ting Cai, Ting Cai   +7 more
doaj   +1 more source

A Field Deployable Real-Time Loop-Mediated Isothermal Amplification Targeting Five Copy nrdB Gene for the Detection of ‘Candidatus Liberibacter asiaticus’ in Citrus [PDF]

The Plant Pathology Journal, 2023
Huanglongbing (HLB) is one of the most destructive diseases in citrus, which imperils the sustainability of citriculture worldwide. The presumed causal agent of HLB, ‘Candidatus Liberibacter asiaticus’ (CLas) is a non-culturable phloem-limited α ...
Tirumalareddy Danda, Jong-Won Park, Kimberly L. Timmons, Mamoudou Sétamou, Eliezer S. Louzada, Madhurababu Kunta   +5 more
doaj   +1 more source

Evaluation and improvement of isothermal amplification methods for point-of-need plant disease diagnostics.

PLoS ONE, 2020
A number of isothermal DNA amplification technologies claim to be ideal for point-of-need (PON) applications as they enable reactions to be performed using a single-temperature heat source (e.g. water bath).
Yiping Zou, Michael Glenn Mason, Jose Ramon Botella   +2 more
doaj   +1 more source

Accelerated loop-mediated isothermal amplification method for the rapid detection of Streptococcus suis serotypes 2 and 14 based on single nucleotide polymorphisms

Frontiers in Cellular and Infection Microbiology, 2022
Streptococcus suis serotypes 2 and 14 are the most prevalent zoonotic strains. The establishment of a sensitive and extremely accurate method for point-of-care testing for Streptococcus suis serotype 2 and 14 strains is highly desirable. In this study, a
Jiajia Meng, Chunling Li, Chunling Li, Chunling Li, Chunling Li, Yu Wang, Zhibiao Bian, Zhibiao Bian, Zhibiao Bian, Zhibiao Bian, Pinpin Chu, Pinpin Chu, Pinpin Chu, Pinpin Chu, Shaolun Zhai, Shaolun Zhai, Shaolun Zhai, Shaolun Zhai, Dongxia Yang, Dongxia Yang, Dongxia Yang, Dongxia Yang, Shuai Song, Shuai Song, Shuai Song, Shuai Song, Yan Li, Yan Li, Yan Li, Yan Li, Zhiyong Jiang, Zhiyong Jiang, Zhiyong Jiang, Zhiyong Jiang, Kunli Zhang, Kunli Zhang, Kunli Zhang, Kunli Zhang, Yugu Li, Hongchao Gou, Hongchao Gou, Hongchao Gou, Hongchao Gou   +42 more
doaj   +1 more source

Loop‐mediated isothermal amplification of single pollen grains [PDF]

Journal of Integrative Plant Biology, 2014
AbstractThe polymerase chain reaction (PCR) has been a reliable and fruitful method for many applications in ecology. Nevertheless, unavoidable technical and instrumental requirements of PCR have limited its widespread application in field situations. The recent development of isothermal DNA amplification methods provides an alternative to PCR, which ...
Ali, Bektaş, Ignacio, Chapela
openaire   +2 more sources

Molecular Methods for Identification and Quantification of Foodborne Pathogens

Molecules, 2022
Foodborne pathogens that enter the human food chain are a significant threat worldwide to human health. Timely and cost-effective detection of them became challenging for many countries that want to improve their detection and control of foodborne ...
Min Zhang, Jiajia Wu, Zhaoai Shi, Aocheng Cao, Wensheng Fang, Dongdong Yan, Qiuxia Wang, Yuan Li   +7 more
doaj   +1 more source

Digital Loop-Mediated Isothermal Amplification on a Commercial Membrane [PDF]

ACS Sensors, 2019
In this work, we report digital loop-mediated isothermal amplification (LAMP) or reverse-transcription LAMP (RT-LAMP) on a commercial membrane, without the need for complex chip fabrication or use of specialized equipment. Due to the pore size distribution, the theoretical error for digital LAMP on these membranes was analyzed, using a combination of ...
Xingyu Lin, Xiao Huang, Katharina Urmann, Xing Xie, Michael R. Hoffmann   +4 more
openaire   +3 more sources

Evaluation of loop-mediated isothermal amplification for detecting COVID-19

Journal of Clinical Virology Plus, 2022
The emergence of SARS-CoV-2 has caused worldwide pandemic of COVID-19. Infection is difficult to diagnose early as some patients remain asymptomatic and may carry this virus to other people. Currently, qRT-PCR is the widely accepted mode for detection.
Astuti Giantini, Muhammad Suhaeri, Ardiana Kusumaningrum, Dimas Seto Prasetyo, Windy Sahar, Rakhmad Hidayat, Muhammad Arza Putra, null Hermawan   +7 more
openaire   +3 more sources