Results 31 to 40 of about 2,335,448 (272)

Head-to-head comparison of two loop-mediated isothermal amplification (LAMP) kits for diagnosis of malaria in a non-endemic setting

Malaria Journal, 2023
Background Light microscopy and rapid diagnostic tests (RDT) have long been the recommended diagnostic methods for malaria. However, in recent years, loop-mediated isothermal amplification (LAMP) techniques have been shown to offer superior performance ...
Anna-Clara Ivarsson, Elin Fransén, Ioanna Broumou, Anna Färnert, Kristina E. M. Persson, Sara Karlsson Söbirk   +5 more
doaj   +1 more source

Loop-mediated isothermal amplification (LAMP) for point-of-care detection of asymptomatic low-density malaria parasite carriers in Zanzibar. [PDF]

, 2015
BACKGROUND: Asymptomatic, low parasite density malaria infections are difficult to detect with currently available point-of-care diagnostics. This study piloted a loop-mediated isothermal amplification (LAMP) kit for field-friendly, high-throughput ...
Msellem, Mwinyi, Abass, Ali K, Mårtensson, Andreas, Schmidt, Berit, Abass, Ali K., Berit Aydin-Schmidt, Mwinyi Msellem, Nassor, Majda H, Cook, Jackie, Aydin-Schmidt, Berit, Nassor, Majda H., Björkman, Anders, Abdullah Ali, Elin Edlund, David Bell, Majda H Nassor, Andreas Mårtensson, Jackie Cook, Ali, Abdullah, Mårtensson, Andreas,, Anders Björkman, Gonzalez, Iveth J., González, Iveth J, Bell, David, Edlund, Elin, Ali K Abass, Iveth J González   +26 more
core   +1 more source

DNA Extraction with DNAzol and LAMP, Performed in a Heating Block as a Simple Procedure for Detection of Mycobacterium tuberculosis in Sputum Specimens

Methods and Protocols, 2018
Tuberculosis (TB) remains as a major public health issue in developing countries. Accurate detection is essential for the proper management of patients with active disease.
Álvaro Rodríguez-García, Rosa E. Mares, Patricia L. A. Muñoz, Samuel G. Meléndez-López, Alexei F. Licea-Navarro, Marco A. Ramos   +5 more
doaj   +1 more source

Electric LAMP: Virtual Loop-Mediated Isothermal AMPlification [PDF]

ISRN Bioinformatics, 2012
We present eLAMP, a PERL script, with Tk graphical interface, that electronically simulates Loop-mediated AMPlification (LAMP) allowing users to efficiently test putative LAMP primers on a set of target sequences. eLAMP can match primers to templates using either exact (via builtin PERL regular expressions) or approximate matching (via the tre-agrep ...
Salinas, Nelson R., Little, Damon P.
openaire   +2 more sources

Nucleic acid extraction-free isothermal amplification based on magnetic beads capture and ligation [PDF]

Jichu yixue yu linchuang, 2021
Objective Capture and ligation probe-loop-mediated isothermal amplification CLIP-LAMP method with-out nucleic acid extraction based on magnetic beads was established to provide a new technique for the detection of human immunodeficiency virus (HIV) in
QIU Li-zhen, YANG Ming-zhu, PIAN Hong-ru, ZHENG Zhi
doaj  

Development of a Novel Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Monkeypox Virus Infections

Viruses, 2022
A recent outbreak of monkeypox virus (mpox) has prompted researchers to explore diagnostics as a means of impeding transmission and further spread. Rapid, sensitive, and specific methods are crucial for accurately diagnosing mpox infections.
Chao Yu, Lulu Zuo, Jing Miao, Lingjing Mao, Benjamin Selekon, Ella Gonofio, Emmanuel Nakoune, Nicolas Berthet, Gary Wong   +8 more
doaj   +1 more source

Assessing the presence of Wuchereria bancrofti in vector and human populations from urban communities in Conakry, Guinea [PDF]

, 2015
The Global Programme to Eliminate Lymphatic Filariasis was launched in 2000 with the goal of interrupting transmission of lymphatic filariasis (LF) through multiple rounds of mass drug administration (MDA).
Bockarie, Moses J., Bockarie, Moses, Utzinger, Jürg, Sandra A. King, Daniel A. Boakye, Andre Goepogui, Goepogui, Andre, Dziedzom K. de Souza, Benjamin G. Koudou, Narh, Charles A., Jürg Utzinger, Koudou, Benjamin, de Souza, Dziedzom K., Mamadou, Baldé S., Kouassi, Bernard L., King, Sandra A., Diakité, Lamia, Koudou, Benjamin G., Moses J. Bockarie, Boakye, Daniel A., Dadzie, Samuel K., Samuel K. Dadzie, Bernard L. Kouassi, Baldé S. Mamadou, Lamia Diakité, Charles A. Narh   +25 more
core   +1 more source

Loop-Mediated Isothermal Amplification: From Theory to Practice

Russian Journal of Bioorganic Chemistry, 2022
Increasing the accuracy of pathogen identification and reducing the duration of analysis remain relevant for modern molecular diagnostics up to this day. In laboratory and clinical practice, detection of pathogens mostly relies on methods of nucleic acid amplification, among which the polymerase chain reaction (PCR) is considered the "gold standard ...
Shirshikov, F. V., Bespyatykh, J. A.
openaire   +2 more sources

Loop-Mediated Isothermal Amplification for Detection of African Trypanosomes [PDF]

Journal of Clinical Microbiology, 2003
ABSTRACTWhile PCR is a method of choice for the detection of African trypanosomes in both humans and animals, the expense of this method negates its use as a diagnostic method for the detection of endemic trypanosomiasis in African countries. The loop-mediated isothermal amplification (LAMP) reaction is a method that amplifies DNA with high specificity,
Noritaka, Kuboki, Noboru, Inoue, Tatsuya, Sakurai, Francescopaolo, Di Cello, Dennis J, Grab, Hiroshi, Suzuki, Chihiro, Sugimoto, Ikuo, Igarashi   +7 more
openaire   +2 more sources

Asymmetric stem-loop–mediated isothermal amplification of nucleic acids for DNA diagnostic assays by simple modification of canonical PCR primers

Frontiers in Bioengineering and Biotechnology, 2022
Nucleic acid–based assays have been adopted as mainstream tools for clinical diagnostics, food safety, and environment monitoring with the merits of accuracy, rapidity, and sensitivity.
Rui Mao, Rui Mao, Xinyao Wu, Xinyao Wu, Qing Miao, Qing Miao, Ting Cai, Ting Cai   +7 more
doaj   +1 more source