Results 61 to 70 of about 2,335,448 (272)

Loop mediated isothermal amplification of Clostridioides difficile isolates in gastrointestinal patients

AMB Express, 2022
This study investigated the prevalence of Clostridioides difficile by culture, multiplex polymerase chain reaction (M-PCR), and loop mediated isothermal amplification (LAMP) in patients with suspected C. difficile infections (CDIs).
Mojtaba Moosavian, Razieyeh Keshavarzi, Effat Abbasi Montazeri, Eskandar Hajiani   +3 more
doaj   +1 more source

Molecular Methods for Identification and Quantification of Foodborne Pathogens

Molecules, 2022
Foodborne pathogens that enter the human food chain are a significant threat worldwide to human health. Timely and cost-effective detection of them became challenging for many countries that want to improve their detection and control of foodborne ...
Min Zhang, Jiajia Wu, Zhaoai Shi, Aocheng Cao, Wensheng Fang, Dongdong Yan, Qiuxia Wang, Yuan Li   +7 more
doaj   +1 more source

Loop-mediated isothermal amplification (LAMP) test for detection of Trypanosoma evansi strain B [PDF]

, 2010
Camel Trypanosomiasis (Surra) is mainly caused by Trypanosoma evansi strains that express variable surface glycoprotein (VSG) RoTat 1.2 However, in Kenya a second causative strain that does not express RoTat 1.2 VSG (T. evansi type B) has been identified.
Njiru, Z.K., Enyaru, J.C., Dargantes, A.P., Ouma, J.O.   +3 more
core  

Nano Borophene as a Programmable 2D Scaffold for Organized DNA Assembly and Long‐Range Energy Transfer

Advanced Functional Materials, EarlyView.
Borophene is introduced as a programmable 2D bio‐interface that organizes DNA through dual boron–sulfur and nucleobase interactions. This molecular architecture enables efficient long‐range nanosurface energy transfer, establishing new design principles for functional nano–bio interfaces and amplification‐free optical biosensing.
Teresa Aditya, Pranay Saha, John M. Sansalone, Shraddha Krishnakumar, Matthew Molinaro, André J. van der Vlies, Nada Maher, Adrienne Griffiths, Zakhro Zakirova, Ketan Dighe, Narayana R. Aluru, Dipanjan Pan   +11 more
wiley   +1 more source

Helix loop-mediated isothermal amplification of nucleic acids

RSC Advances, 2018
In this study, a helix loop-mediated isothermal amplification (HAMP) method with high specificity, efficiency and rapidity was developed.
Rui Mao, Lifei Qi, Zhuo Wang, Hongtao Liu, Yuguang Du   +4 more
openaire   +3 more sources

Detection of Pathogen-Specific Antibodies by Loop-Mediated Isothermal Amplification [PDF]

Clinical and Vaccine Immunology, 2015
ABSTRACTLoop-mediated isothermal amplification (LAMP) is a method for enzymatically replicating DNA that has great utility for clinical diagnosis at the point of care (POC), given its high sensitivity, specificity, speed, and technical requirements (isothermal conditions).
Burbulis, Ian, Yamaguchi, Kumiko, Nikolskaia, Olga, Prigge, Sean, Magez, Stefan, Bisser, Sylvie, Reller, Megan, Grab, Dennis   +7 more
openaire   +5 more sources

Detection of Mycobacterium ulcerans by the loop mediated isothermal amplification method. [PDF]

PLoS Neglected Tropical Diseases, 2012
BACKGROUND: Buruli ulcer (BU) caused by Mycobacterium ulcerans (M. ulcerans) has emerged as an important public health problem in several rural communities in sub-Saharan Africa.
Anthony Ablordey, Diana Ackon Amissah, Isaac Frimpong Aboagye, Ben Hatano, Toshio Yamazaki, Tetsutaro Sata, Koichi Ishikawa, Harutaka Katano   +7 more
doaj   +1 more source

Nitride MXenes Beyond Carbides: Bridging the Gap Between Computational Prediction and Experimental Realization

Advanced Functional Materials, EarlyView.
Nitride MXenes remain constrained by a persistent gap between computational prediction and experimental realization. This Review identifies the thermodynamic, kinetic, and chemical barriers limiting their synthesis, critically evaluates emerging fabrication routes, and proposes a multidimensional computational‐experimental framework to accelerate the ...
Naresh Varnakavi, Masoud Soroush
wiley   +1 more source

Real-time fluorescence loop mediated isothermal amplification for the diagnosis of malaria. [PDF]

PLoS ONE, 2010
BACKGROUND: Molecular diagnostic methods can complement existing tools to improve the diagnosis of malaria. However, they require good laboratory infrastructure thereby restricting their use to reference laboratories and research studies.
Naomi W Lucchi, Allison Demas, Jothikumar Narayanan, Deborah Sumari, Abdunoor Kabanywanyi, S Patrick Kachur, John W Barnwell, Venkatachalam Udhayakumar   +7 more
doaj   +1 more source

Attomolar Detection of HIV‐1 With Label‐Free RCA‐rCRISPR on Smartphone

Advanced Science, EarlyView.
A new diagnostic strategy integrating rolling circle amplification (RCA) with a plasmid reporter–based ratiometric CRISPR (rCRISPR) system was developed to detect unfragmented HIV genomes with single‐digit attomolar sensitivity. The method quantifies targets using the plasmid relaxation ratio and employs a smartphone‐based mini‐gel device to enable ...
Noor Mohammad, Anastasiia Steksova, Yuyang Tang, Leyao Huang, Alireza Velayati, Shengwei Zhang, Aditi Dey Poonam, Sina Jamalzadegan, Matthew Breen, Hongjie Chen, Mahsa Bagi, Guochun Jiang, Qingshan Wei   +12 more
wiley   +1 more source