Results 61 to 70 of about 48,371 (318)

Asymmetric stem-loop–mediated isothermal amplification of nucleic acids for DNA diagnostic assays by simple modification of canonical PCR primers

Frontiers in Bioengineering and Biotechnology, 2022
Nucleic acid–based assays have been adopted as mainstream tools for clinical diagnostics, food safety, and environment monitoring with the merits of accuracy, rapidity, and sensitivity.
Rui Mao, Rui Mao, Xinyao Wu, Xinyao Wu, Qing Miao, Qing Miao, Ting Cai, Ting Cai   +7 more
doaj   +1 more source

Multiplex reverse transcription loop-mediated isothermal amplification combined with nanoparticle-based lateral flow biosensor for the diagnosis of COVID-19

Biosensors & bioelectronics, 2020
The ongoing global pandemic (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a huge public health issue.
Xiong Zhu, Xiaoxia Wang, Limei Han, Ting Chen, Licheng Wang, Huan Li, Sha Li, Lvfen He, Xiaoying Fu, Shaojin Chen, M. Xing, Hai Chen, Yi Wang   +12 more
semanticscholar   +1 more source

Development of a Loop-Mediated Isothermal Amplification Method for Rapid and Visual Detection of Monkeypox Virus

Microbiology spectrum, 2022
MPXV outbreaks rapidly grew in the first half of 2022, and this virus has been recognized as an increasing public health threat, particularly in the context of the COVID-19 pandemic.
Junxia Feng, Guan-hua Xue, X. Cui, B. Du, Yanling Feng, J. Cui, Hanqing Zhao, Lin Gan, Zheng Fan, Tongtong Fu, Ziyi Xu, Shuheng Du, Yao Zhou, Rui Zhang, Hanyu Fu, Ziyan Tian, Qun Zhang, Chao Yan, Jing Yuan   +18 more
semanticscholar   +1 more source

Loop‐mediated isothermal amplification of single pollen grains [PDF]

Journal of Integrative Plant Biology, 2014
AbstractThe polymerase chain reaction (PCR) has been a reliable and fruitful method for many applications in ecology. Nevertheless, unavoidable technical and instrumental requirements of PCR have limited its widespread application in field situations. The recent development of isothermal DNA amplification methods provides an alternative to PCR, which ...
Ali, Bektaş, Ignacio, Chapela
openaire   +2 more sources

Accelerated loop-mediated isothermal amplification method for the rapid detection of Streptococcus suis serotypes 2 and 14 based on single nucleotide polymorphisms

Frontiers in Cellular and Infection Microbiology, 2022
Streptococcus suis serotypes 2 and 14 are the most prevalent zoonotic strains. The establishment of a sensitive and extremely accurate method for point-of-care testing for Streptococcus suis serotype 2 and 14 strains is highly desirable. In this study, a
Jiajia Meng, Chunling Li, Chunling Li, Chunling Li, Chunling Li, Yu Wang, Zhibiao Bian, Zhibiao Bian, Zhibiao Bian, Zhibiao Bian, Pinpin Chu, Pinpin Chu, Pinpin Chu, Pinpin Chu, Shaolun Zhai, Shaolun Zhai, Shaolun Zhai, Shaolun Zhai, Dongxia Yang, Dongxia Yang, Dongxia Yang, Dongxia Yang, Shuai Song, Shuai Song, Shuai Song, Shuai Song, Yan Li, Yan Li, Yan Li, Yan Li, Zhiyong Jiang, Zhiyong Jiang, Zhiyong Jiang, Zhiyong Jiang, Kunli Zhang, Kunli Zhang, Kunli Zhang, Kunli Zhang, Yugu Li, Hongchao Gou, Hongchao Gou, Hongchao Gou, Hongchao Gou   +42 more
doaj   +1 more source

Diagnostic accuracy of TB-LAMP for pulmonary tuberculosis: a systematic review and meta-analysis. [PDF]

, 2019
BACKGROUND:The need for a rapid, molecular test to diagnose tuberculosis (TB) has prompted exploration of TB-LAMP (Eiken; Tokyo, Japan) for use in resource-limited settings.
Cattamanchi, Adithya, Farr, Katherine, Gray, Christen M, Shete, Priya B, Strnad, Luke   +4 more
core   +2 more sources

A Field Deployable Real-Time Loop-Mediated Isothermal Amplification Targeting Five Copy nrdB Gene for the Detection of ‘Candidatus Liberibacter asiaticus’ in Citrus [PDF]

The Plant Pathology Journal, 2023
Huanglongbing (HLB) is one of the most destructive diseases in citrus, which imperils the sustainability of citriculture worldwide. The presumed causal agent of HLB, ‘Candidatus Liberibacter asiaticus’ (CLas) is a non-culturable phloem-limited α ...
Tirumalareddy Danda, Jong-Won Park, Kimberly L. Timmons, Mamoudou Sétamou, Eliezer S. Louzada, Madhurababu Kunta   +5 more
doaj   +1 more source

Evaluation of loop-mediated isothermal amplification for detecting COVID-19

Journal of Clinical Virology Plus, 2022
The emergence of SARS-CoV-2 has caused worldwide pandemic of COVID-19. Infection is difficult to diagnose early as some patients remain asymptomatic and may carry this virus to other people. Currently, qRT-PCR is the widely accepted mode for detection.
Astuti Giantini, Muhammad Suhaeri, Ardiana Kusumaningrum, Dimas Seto Prasetyo, Windy Sahar, Rakhmad Hidayat, Muhammad Arza Putra, null Hermawan   +7 more
openaire   +3 more sources

Development of a reverse transcription-loop-mediated isothermal amplification as a rapid early-detection method for novel SARS-CoV-2

Emerging Microbes and Infections, 2020
The previous outbreaks of SARS-CoV and MERS-CoV have led researchers to study the role of diagnostics in impediment of further spread and transmission. With the recent emergence of the novel SARS-CoV-2, the availability of rapid, sensitive, and reliable ...
Y. Baek, Jihye Um, K. J. C. Antigua, Ji-Hyun Park, Yeonjae Kim, Sol-La Oh, Young-Il Kim, Won-Suk Choi, Seonggyu Kim, J. Jeong, B. Chin, H. D. G. Nicolas, Ji-Young Ahn, K. Shin, Y. Choi, Jun-Sun Park, Min-Suk Song   +16 more
semanticscholar   +1 more source

Loop-mediated isothermal amplification for the detection of goose circovirus [PDF]

Virology Journal, 2012
Abstract Background Goose circovirus (GCV) presents an immunosuppressive problem in production of geese. The infection’s clinical symptoms include growth retardation or feathering disorders but the infection process may remain non-symptomatic what makes the infected birds more susceptible for secondary viral ...
Woźniakowski Grzegorz, Kozdruń Wojciech, Samorek-Salamonowicz Elżbieta   +2 more
openaire   +3 more sources