Results 191 to 200 of about 2,335,448 (272)

Comparative evaluation of 14 <i>hlyA</i>-based loop-mediated isothermal amplification assays for the rapid detection of <i>Listeria monocytogenes</i>. [PDF]

Front Microbiol
Mallmann L, Ge B, Springer M, Domesle KJ, de Vasconcellos V, Chen Y, Ximenes E.   +6 more
europepmc   +1 more source

Synergistic Integration of Recombinase Polymerase Amplification and CRISPR–Cas12a Enables High‐Sensitivity Detection of Mycoplasma bovis

MicrobiologyOpen, Volume 15, Issue 5, October 2026.
This graphical abstract illustrates a rapid diagnostic platform for Mycoplasma bovis detection using Recombinase Polymerase Amplification coupled with CRISPR–Cas12a targeting the uvrC gene. The assay operates at 37°C–39°C, delivers results within 1 h, and demonstrates complete concordance with PCR in cattle and bison clinical samples.
Diba Yaghoubi, Viju V. Pillai
wiley   +1 more source

Establishment of a loop-mediated isothermal amplification-lateral flow dipstick assay for the point-of-care testing of feline herpesvirus-1. [PDF]

Front Vet Sci
Yi S, Zhao H, Huang J, Cai Y, Wu J, Lian W, Niu J, Yin B.   +7 more
europepmc   +1 more source

A dual gating mechanism controls target‐strand cleavage in Cas12j: Implications for engineering efficient nickases

Protein Science, Volume 35, Issue 10, October 2026.
Abstract The rapid expansion of CRISPR technologies has unveiled a diverse repertoire of RNA‐guided endonucleases, among them the compact Cas12j, which has emerged as a promising genome‐editing tool. Cas12j cleaves the two DNA strands sequentially; however, the molecular mechanism that regulates this cleavage remains incompletely understood.
Ilias G. Karvounis, Vangelis Daskalakis
wiley   +1 more source

Loop-Mediated Isothermal Amplification (LAMP) Assay for the Detection of Bacterial Bovine Mastitis: A Systematic Review. [PDF]

Vet Med Sci
Jember TF, Westman ME, Pant SD, Ghorashi SA.   +3 more
europepmc   +1 more source

Strain‐Dependent Evolution of Fatigue and Oxidation Damage in a Nickel‐Based Single‐Crystal Superalloy: The Dominant Mechanism Under Low‐Cycle and Thermomechanical Fatigue

Rare Metals, Volume 45, Issue 10, October 2026.
ABSTRACT Systematic studies on the fatigue deformation and damage mechanisms of nickel‐based single‐crystal superalloys (Ni‐SXs) under combined thermal and mechanical conditions remain limited, particularly regarding the influence of strain amplitude on the relative contributions of fatigue and oxidation damage.
Liyin Zhang, Xiaoqing Liang, Xiaofeng Dang, Zhenyang Cao, Hao Su, Sihai Luo, Luqing Cui, Weifeng He   +7 more
wiley   +1 more source

Development of a Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Molecular Identification of the Bark Beetle <i>Ips hauseri</i> (Coleoptera: Curculionidae). [PDF]

Insects
Jiao J, Zhang W, Yan L, Zhou Y, Li S, Ren L, Sattar A.   +6 more
europepmc   +1 more source

Sodium Channel Blockers Demonstrate Binding Affinity for the Tandem Tudor Domain of the Epigenetic Hub UHRF1

ChemMedChem, Volume 21, Issue 17, 14 September 2026.
An investigation of the tandem Tudor domain (TTD) domain of UHRF1 identified and extensively characterized the local anesthetic hydroxyprocaine as a strong ligand capable of activating related genes such as p53 and also highlighted a new, potentially druggable intramolecular interaction between TTD and UBL.
Efstratios Tsakalidis, Mariangela Giannouli, Ifigeneia Akrani, Anthi Panara, Eleni Katalagarianou, Kyriaki Degaita, Maria Giahou, Konstantina Kyriakou, Maria Routsi, Panagiota Efstathia Nikolaou, Evangelia Tsitsou, Aggeliki Tsoka, Constantinos C. Vorgias, Emmanuel Mikros, Evangelos Gikas, Eleni K. Efthimiadou, Vassilios Myrianthopoulos   +16 more
wiley   +1 more source

Single-Tube Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Red-Spotted Grouper Nervous Necrosis Virus in Fish Species. [PDF]

Viruses
Pradeep MA, Subin CS, Kumar G, Sharma SRK, Sanil NK, Arun Kumar TV, Dhanutha NR, Shahansha TSA, Vijayan KK.   +8 more
europepmc   +1 more source

A loop-mediated isothermal amplification test for yaws

Background: To meet the WHO target of eradicating yaws by 2030, highly sensitive and specific diagnostic tools are needed. A multiplex Treponema pallidum-Haemophilus ducreyi loop-mediated isothermal amplification (TPHD-LAMP) test holds promise as a near-patient diagnostic tool for yaws and H ducreyi.
Handley, Becca L., González-Beiras, Camila||, Tchatchouang, Serges||, Hugues, Kouadio Aboh, Basing, Laud Antony, Sylla, Aboubacar, Kouamé-Sina, Mireille S., Amanor, Ivy, Ndzomo, Philippe, Aloumba, Axel, Bakheit, Mohammed, Müller, Claudia, Borst, Nadine, Landmann, E., Gmoser, H., Härpfer, T., Becherer, L., Lüert, S., Frischmann, S., Burl, S., Tabah, E.N., Crucitti, Tania, Kouadio, A.T., Arhinful, D.K., Awondo, P., Kakou, S.N., Eyangoh, S., Addo, Kennedy Kwasi, Knauf, S., Mitjà, Oriol||, Harding-Esch, E.M., Marks, M.   +31 more
openaire   +1 more source