Results 191 to 200 of about 48,371 (318)

Development of PCR, LAMP and HRM‐Based Molecular Species Diagnostic Tools for Identifying Four Agricultural Pests in the Genus Helicoverpa (Lepidoptera: Noctuidae)

Journal of Applied Entomology, EarlyView.
ABSTRACT Within the subfamily Heliothinae, the genus Helicoverpa comprises several highly destructive agricultural pests, including H. armigera, H. assulta, H. punctigera and H. zea. These species cause significant global crop losses due to their wide host range, long‐distance migratory behaviour, and potential for interspecific hybridization.
Minseop Noh, Murtaza Khan, Minyoung Choi, Andreas Zwick, Juil Kim   +4 more
wiley   +1 more source

A multiplex assay to detect mosquito species, bloodmeal host source and Plasmodium in malaria vectors using Nanopore amplicon sequencing

Medical and Veterinary Entomology, EarlyView.
Insectary‐reared mosquitoes were simultaneously assessed for species identification, bloodmeal source analysis and Plasmodium detection, using a single multiplexed sequencing run on the MinION sequencing platform. The method was applied to 150 insectary‐reared mosquitoes, representing nine species blood‐fed with five vertebrate hosts and 40 samples for
E. Abby Rogers, Dieunel Derilus, Christopher Sandi, Lisa Reimer, Audrey Lenhart, Lucy Mackenzie Impoinvil   +5 more
wiley   +1 more source

Deciphering Cold Stress Resilience: Multiomics Insights in Contrasting Wheat Genotypes From the Western Himalayas

Plant Biotechnology Journal, EarlyView.
ABSTRACT Cold stress threatens wheat productivity, particularly in regions with extreme climatic conditions. To elucidate the molecular mechanisms underlying wheat's response to cold stress, we performed a multiomics analysis integrating lipidomics, transcriptomics, proteomics and metabolomics.
Sofora Jan, Farkhandah Jan, Mukesh Rathore, Yogita Singh, Prexha Kapoor, Palak Chaturvedi, Arindam Ghatak, Palakurthi Ramesh, Upendra Kumar, Manoj Prasad, Sundeep Kumar, Sachin Rustgi, Wolfram Weckwerth, Sanjay Kalia, Rajeev Kumar Varshney, Reyazul Rouf Mir   +15 more
wiley   +1 more source

Technologies for engineering repetitive DNA

Quantitative Biology, Volume 14, Issue 3, September 2026.
Abstract Repetitive DNA, a fundamental architectural element of genomes, is widespread across organisms and comprises about 54% of the human genome. With advances in long‐read sequencing and bioinformatics approaches, highly repetitive sequences can now be characterized in depth.
Shuting Ma, Yali Cui, Yi Wu
wiley   +1 more source

Polymerase-endonuclease amplification reaction for large-scale enzymatic production of antisense oligonucleotide [PDF]

, 2009
Synthetic oligonucleotides are contaminated with highly homologous failure sequences. Oligonucleotide synthesis is difficult to scale up because it requires expensive equipments, hazardous chemicals, and tedious purification process.
Deming Gou, Xiaolong Wang
core   +1 more source

Multiplexed microfluidic loop-mediated isothermal amplification of the 16s rRNA gene for the diagnosis of neonatal sepsis in resource-limited environments [PDF]

, 2015
Bacterial infection, or sepsis, places a disproportionately high burden on newborns in developing countries. This is due in part to a lack of diagnostic tools suitable for sustainable use in resource-limited nurseries.
Sonaty, Griffin Thomas
core   +1 more source

Light‐Activated Nucleic Acid Amplification Systems Using Photo‐Caged DNA Polymerase or Primers

Advanced Genetics, Volume 7, Issue 2, June 2026.
ABSTRACT Precise control over nucleic acid amplification is essential for achieving reliable, quantitative, high‐throughput, and multiplexed molecular diagnostics, particularly in point‐of‐care and field settings. However, conventional isothermal amplification methods, especially rapid assays at ambient temperature such as recombinase‐aided ...
Yang Wu, Meng Wu, Yukun Tian, Hao Chen, Xizhe Sun, Chukang Ma, Jiayao Liu, Fuyu Xia, Yu Liu, Xiaomeng Pei, Jing Wang, Guogang Zhao, Nan Wang, Fangfang Wang, Qing Liu, Fei Yu, Xiaofei Fan, Zehe Wang, Elena Ermilova, Jon S. West, Qi Cheng   +20 more
wiley   +1 more source

Innovative Drug Delivery Systems for the Treatment of Glaucoma

MedComm – Biomaterials and Applications, Volume 5, Issue 2, June 2026.
This review aims to provide a comprehensive overview of current topical drug delivery strategies for ocular hypotensive agents in glaucoma therapy. Additionally, it discusses the potential impact of emerging nanotechnological and biomaterial‐based systems in shaping the future of ophthalmic drug delivery, with an emphasis on enhancing efficacy, safety,
Jing Chu, Junzhi Li, Zhenlin Chen, Jinyu Zhang, Siqi Feng, Jiaying Huang, Yawen Chu, Xue Zhou, Wei Wang, Shangsi Chen   +9 more
wiley   +1 more source

Loop-mediated isothermal amplification for detection of Legionella pneumophila in respiratory specimens of hospitalized patients in Ahvaz, southwest Iran

Infection and Drug Resistance, 2019
Mojtaba Moosavian,1,2 Sakineh Seyed-Mohammadi,2,3 Morteza Saki,2,3 Fatemeh Shahi,2,3 Mahtab Khoshkholgh Sima,2 Davoud Afshar,4 Sara Barati5 1Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of ...
Moosavian M, Seyed-Mohammadi S, Saki M, Shahi F, Khoshkholgh Sima M, Afshar D, Barati S   +6 more
doaj  

Functional dynamics of water in New Delhi metallo‐β‐lactamase catalysis

Protein Science, Volume 35, Issue 6, June 2026.
Abstract Water plays a critical role in the hydrolysis of antibiotics by New Delhi metallo‐β‐lactamases (NDMs). The reaction proceeds through a nucleophilic attack on the β‐lactam ring, followed by cleavage of the C–N bond and formation of an anionic nitrogen intermediate. This intermediate is then protonated by a water molecule diffusing from the bulk
Palanisamy Kandhan, Chuanye Xiong, Timothy Palzkill, Peng Tao   +3 more
wiley   +1 more source