Results 231 to 240 of about 35,344 (268)

Applications of Loop-Mediated Isothermal DNA Amplification

Applied Biochemistry and Biotechnology, 2010
During the last 10 years, with the development of loop-mediated isothermal amplification (LAMP) method, it has been widely applied in nucleic acid analysis because of its simplicity, rapidity, high efficiency, and outstanding specificity. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six ...
Shijun, Fu, Guanggang, Qu, Shijin, Guo, Lin, Ma, Na, Zhang, Songlin, Zhang, Sanyang, Gao, Zhiqiang, Shen   +7 more
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Hendra virus detection using Loop-Mediated Isothermal Amplification

Journal of Virological Methods, 2012
(Uploaded by Plazi for the Bat Literature Project) No abstract provided.
Adam J, Foord, Deborah, Middleton, Hans G, Heine   +2 more
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Detection of porcine parvovirus by loop-mediated isothermal amplification

Journal of Virological Methods, 2009
Loop-mediated isothermal amplification is a novel method for rapid amplification of DNA. It has been adopted widely for the detection of virus because of its simplicity, rapidity, and specificity. A loop-mediated isothermal amplification assay was developed for the detection of porcine parvovirus.
Chang-mu, Chen, Shang-jin, Cui
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Detection of fish nocardiosis by loop-mediated isothermal amplification

Journal of Applied Microbiology, 2006
Loop-mediated isothermal amplification (LAMP) is a novel method that amplifies DNA with high specificity and rapidity under isothermal conditions. In this study, using the LAMP method, a protocol for detecting Nocardia seriolae which is a causative agent of fish nocardiosis, was designed.A set of four primers, two inner and two outer, were designed ...
T, Itano, H, Kawakami, T, Kono, M, Sakai
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Colorimetric strategies applicable for loop-mediated isothermal amplification

Journal of Microbiological Methods
In recent years, loop-mediated isothermal amplification (LAMP) has gained popularity for detecting various pathogen-specific genes due to its superior sensitivity and specificity compared to conventional polymerase chain reaction (PCR). The simplicity and flexibility of naked-eye detection of the amplicon make LAMP an ideal rapid and straightforward ...
Syaidatul Akmal, Saifuddin, Roslina, Rashid, Nurin Jazlina, Nor Azmi, Suharni, Mohamad   +3 more
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Loop-mediated isothermal amplification in disposable polyester-toner microdevices

Analytical Biochemistry, 2017
In recent years, isothermal DNA amplification methods have emerged as an alternative in molecular diagnostics due to its ease of operation. The purpose of using isothermal amplification is to simplify the diagnostics work by i) eliminating heating cycles, ii) reducing equipment costs, and iii) providing rapid and accurate results in laboratories with ...
Kezia Gomes de Oliveira, Juliane Cristina Borba, Alexandre Melo Bailão, Célia Maria de Almeida Soares, Emanuel Carrilho, Gabriela Rodrigues Mendes Duarte   +5 more
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Loop‐Mediated Isothermal Amplification for Detection of Nucleic Acids

Current Protocols in Molecular Biology, 2014
AbstractSequence‐specific isothermal nucleic acid amplification techniques are ideally suited for use in molecular diagnostic applications because they do not require thermal cycling equipment and the reactions are typically fast. One of the most widely cited isothermal techniques is termed loop‐mediated isothermal amplification (LAMP).
Nathan A, Tanner, Thomas C, Evans
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In-Field Diagnostics Using Loop-Mediated Isothermal Amplification

2012
Loop-mediated isothermal amplification (LAMP) is a method for amplification and detection of target organisms which, unlike polymerase chain reaction, does not require thermal cycling. LAMP assays can be developed in the laboratory for subsequent deployment in the field, where the simplicity of isothermal amplification makes LAMP a suitable method for ...
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[Loop-mediated isothermal amplification].

Nihon rinsho. Japanese journal of clinical medicine, 2007
We have developed the loop-mediated isothermal amplification (LAMP) method, an innovative gene amplification technique, which has the potential to become important in genetic testing. This method allows highly efficient amplification by a single enzyme at a constant temperature. It also has features such as high specificity, rapidity, and simplicity of
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Visualization methods for loop mediated isothermal amplification (LAMP) assays

The Analyst
This review explores advancements in LAMP assay visualization techniques, highlighting colorimetric, fluorescence, and nanoparticle-based methods that enhance sensitivity, reliability, and field-deployable nucleic acid detection.
Vinni Thekkudan Novi, Anil Kumar Meher, Abdennour Abbas   +2 more
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