Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics [PDF]
Nature Communications, 2019 Spatial proteomics allows studying cellular protein localisations at system-wide scale. Here, the authors show that combining the previously developed hyperLOPIT method with differential centrifugation yields protein localisation maps at suborganellar ...
Oliver Crook +2 more
exaly +5 more sources
Pattern Analysis of Organellar Maps for Interpretation of Proteomic Data [PDF]
Proteomes, 2022 Localization of organelle proteins by isotope tagging (LOPIT) maps are a coordinate-directed representation of proteome data that can aid in biological interpretation.
Jordan B. Burton +4 more
doaj +3 more sources
CFTR Rescue by Lumacaftor (VX-809) Induces an Extensive Reorganization of Mitochondria in the Cystic Fibrosis Bronchial Epithelium [PDF]
Cells, 2022 Background: Cystic Fibrosis (CF) is a genetic disorder affecting around 1 in every 3000 newborns. In the most common mutation, F508del, the defective anion channel, CFTR, is prevented from reaching the plasma membrane (PM) by the quality check control of
Clarissa Braccia +12 more
doaj +2 more sources
Multilocation proteins in organelle communication: Based on protein–protein interactions
Plant Direct, 2022 Protein–protein interaction (PPI) plays a crucial role in most biological processes, including signal transduction and cell apoptosis. Importantly, the knowledge of PPIs can be useful for identification of multimeric protein complexes and elucidation of ...
Erhui Xiong +7 more
doaj +2 more sources
Spatial proteomics of vesicular trafficking: coupling mass spectrometry and imaging approaches in membrane biology. [PDF]
Plant Biotechnol J, 2023 Summary In plants, membrane compartmentalization requires vesicle trafficking for communication among distinct organelles. Membrane proteins involved in vesicle trafficking are highly dynamic and can respond rapidly to changes in the environment and to cellular signals.
Zhang L +8 more
europepmc +2 more sources
Spatiotemporal proteomic profiling of the pro-inflammatory response to lipopolysaccharide in the THP-1 human leukaemia cell line [PDF]
Nature Communications, 2021 “Protein relocalisation plays a major role in the innate immune response but remains incompletely characterised. Here, the authors combine temporal proteomics with LOPIT, a spatial proteomic workflow, in a fully Bayesian framework to elucidate ...
Claire M. Mulvey +12 more
doaj +2 more sources
An updated Bioconductor workflow for correlation profiling subcellular proteomics [version 1; peer review: 2 approved] [PDF]
F1000ResearchBackground Subcellular localisation is a determining factor of protein function. Mass spectrometry-based correlation profiling experiments facilitate the classification of protein subcellular localisation on a proteome-wide scale.
Lisa M Breckels +5 more
doaj +2 more sources
Proteomic dissection of the Arabidopsis Golgi and trans-Golgi network
Frontiers in Plant Science, 2013 The plant Golgi apparatus and trans-Golgi network are major functional hubs within the plant cell and are involved in a diverse and vital series of functions to maintain plant growth and development.
Joshua L Heazlewood +2 more
exaly +3 more sources
Jonathan Moodie & Rosey Billington, A grammar of Lopit: An Eastern Nilotic language of South Sudan
Linguistique et langues africaines, 2022 Lameen Souag
exaly +3 more sources
Reduced mitochondria provide an essential function for the cytosolic methionine cycle
Current Biology, 2022 Sebastian Cristian Treitli +2 more
exaly +3 more sources