Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics [PDF]
Nature Communications, 2019 Spatial proteomics allows studying cellular protein localisations at system-wide scale. Here, the authors show that combining the previously developed hyperLOPIT method with differential centrifugation yields protein localisation maps at suborganellar ...
Aikaterini Geladaki +2 more
exaly +9 more sources
Pattern Analysis of Organellar Maps for Interpretation of Proteomic Data [PDF]
Proteomes, 2022 Localization of organelle proteins by isotope tagging (LOPIT) maps are a coordinate-directed representation of proteome data that can aid in biological interpretation.
Jordan B Burton +2 more
exaly +5 more sources
Conditional constructions in Lopit, an Eastern Nilotic language
Studies in African Linguistics, 2017 Lopit is an Eastern Nilotic language of South Sudan. It has a number of ways of expressing conditionals. The most common way involves the use of the subordinate clause marker l- on the clause-initial verb which introduces the protasis.
Jonathan Moodie
doaj +5 more sources
Jonathan Moodie & Rosey Billington, A grammar of Lopit: An Eastern Nilotic language of South Sudan
Linguistique et langues africaines, 2022 Lameen Souag
exaly +4 more sources
CFTR Rescue by Lumacaftor (VX-809) Induces an Extensive Reorganization of Mitochondria in the Cystic Fibrosis Bronchial Epithelium [PDF]
Cells, 2022 Background: Cystic Fibrosis (CF) is a genetic disorder affecting around 1 in every 3000 newborns. In the most common mutation, F508del, the defective anion channel, CFTR, is prevented from reaching the plasma membrane (PM) by the quality check control of
Clarissa Braccia +12 more
doaj +2 more sources
Multilocation proteins in organelle communication: Based on protein–protein interactions
Plant Direct, 2022 Protein–protein interaction (PPI) plays a crucial role in most biological processes, including signal transduction and cell apoptosis. Importantly, the knowledge of PPIs can be useful for identification of multimeric protein complexes and elucidation of ...
Erhui Xiong +7 more
doaj +2 more sources
Spatial proteomics of vesicular trafficking: coupling mass spectrometry and imaging approaches in membrane biology. [PDF]
Plant Biotechnol J, 2023 Summary In plants, membrane compartmentalization requires vesicle trafficking for communication among distinct organelles. Membrane proteins involved in vesicle trafficking are highly dynamic and can respond rapidly to changes in the environment and to cellular signals.
Zhang L +8 more
europepmc +2 more sources
Spatiotemporal proteomic profiling of the pro-inflammatory response to lipopolysaccharide in the THP-1 human leukaemia cell line [PDF]
Nature Communications, 2021 “Protein relocalisation plays a major role in the innate immune response but remains incompletely characterised. Here, the authors combine temporal proteomics with LOPIT, a spatial proteomic workflow, in a fully Bayesian framework to elucidate ...
Claire M. Mulvey +12 more
doaj +2 more sources
Localization of Organelle Proteins by Isotope Tagging (LOPIT) [PDF]
Molecular and Cellular Proteomics, 2004 We describe a proteomics method for determining the subcellular localization of membrane proteins. Organelles are partially separated using centrifugation through self-generating density gradients. Proteins from each organelle co-fractionate and therefore exhibit similar distributions in the gradient.
J L Griffin +2 more
exaly +3 more sources
Putative Glycosyltransferases and Other Plant Golgi Apparatus Proteins Are Revealed by LOPIT Proteomics [PDF]
Plant Physiology, 2012 AbstractThe Golgi apparatus is the central organelle in the secretory pathway and plays key roles in glycosylation, protein sorting, and secretion in plants. Enzymes involved in the biosynthesis of complex polysaccharides, glycoproteins, and glycolipids are located in this organelle, but the majority of them remain uncharacterized. Here, we studied the
Nino Nikolovski +2 more
exaly +4 more sources