Results 191 to 200 of about 10,942 (259)

Metabolically robust autoluminescent reporters

Morozov VV, Balakireva AV, Gao F, Fleiss A, Mishina NM, Perfilov MM, Mitiouchkina TY, Gorbachev DA, Wood KV, Yampolsky IV, Sarkisyan KS, Mishin AS.   +11 more
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Split Reporter Systems in Viral Protein-Protein Interactions and Multimerization: Mechanisms and Applications. [PDF]

Cells
Ahmad H, Masood F, Iqbal U, Shaltout M, Yukselten Y, Sutton RE.   +5 more
europepmc   +1 more source

A luminescent calcium biosensor enabling endpoint measurement of GPCR-mediated calcium signaling. [PDF]

Commun Biol
Doi K, Kise R, Shimizume K, Yanagawa M, Inoue A.   +4 more
europepmc   +1 more source

Advances in BRET probes for intracellular target engagement studies. [PDF]

Nat Chem Biol
Capener JL, Schwalm MP, Vasta JD, Michaud A, Teske KA, Marsiglia WM, Huber KVM, Dar AC, Knapp S, Axtman AD, Robers MB.   +10 more
europepmc   +1 more source

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Bioluminescence assays: multicolor luciferase assay, secreted luciferase assay and imaging luciferase assay

Expert Opinion on Drug Discovery, 2010
By selecting the most appropriate bioluminescence assay, the researcher can study the underlying molecular mechanisms of a physiological system and the effects of a drug throughout the body.This review covers three luciferase assay systems: the multicolor luciferase assay, secreted luciferase assay and imaging luciferase assay. These assays are applied
Yoshihiro, Nakajima, Yoshihiro, Ohmiya
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Structure of bacterial luciferase

Current Opinion in Structural Biology, 1995
The generation of light by living organisms such as fireflies, glow-worms, mushrooms, fish, or bacteria growing on decaying materials has been a subject of fascination throughout the ages, partly because it occurs without the need for high temperatures.
T O, Baldwin, J A, Christopher, F M, Raushel, J F, Sinclair, M M, Ziegler, A J, Fisher, I, Rayment   +6 more
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Luciferase Assay

Cold Spring Harbor Protocols, 2010
INTRODUCTIONWhen a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because transfection efficiency in such assays can be low, promoters are commonly fused to heterologous reporter genes that encode enzymes that can be quantified using highly sensitive assays. The reporter protein’
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Firefly luciferase inhibition

Journal of Photochemistry and Photobiology B: Biology, 2010
Firefly luciferase (Luc) is the most studied of the luciferase enzymes and the mechanism and kinetics of the reactions catalyzed by this enzyme have been relatively well characterized. Luc catalyzes the bioluminescent reaction involving firefly luciferin (D-LH(2)), adenosine triphosphate (ATP), magnesium ion and molecular oxygen with the formation of ...
João M. M. Leitão, Joaquim C. G. Esteves da Silva   +1 more
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Crystalline firefly luciferase

Biochimica et Biophysica Acta, 1956
Abstract 1. 1. The method for purification and crystallization of firefly luciferase is described. 2. 2. The crystalline enzyme is apparently homogenous as evidenced by ultracentrifugation a and electrophoretic determinations. The molecular weight is probably around 100,000 and the isoelectric point between pH 6.2 and 6.3. 3. 3.
A A, GREEN, W D, MCELROY
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Coelenterazine-dependent luciferases

Biochemistry (Moscow), 2015
Bioluminescence is a widespread natural phenomenon. Luminous organisms are found among bacteria, fungi, protozoa, coelenterates, worms, molluscs, insects, and fish. Studies on bioluminescent systems of various organisms have revealed an interesting feature - the mechanisms underlying visible light emission are considerably different in representatives ...
S V, Markova, E S, Vysotski
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