Results 201 to 210 of about 10,942 (259)

Luciferases with Tunable Emission Wavelengths

Angewandte Chemie - International Edition, 2017
AbstractWe introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The luciferases are chimeras of NanoLuc with either SNAP‐tag or HaloTag7. Labeling of the self‐labeling tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore.
Luc Reymond, Qiuliyang Yu, Lin Xue
exaly   +6 more sources

Luciferases as Reporter Genes

2006
Luciferase is the ideal reporter gene to provide temporal and spatial information on promoter activity in Arabidopsis and other eukaryotes; the noninvasive detection of luminescence and short half-life of luciferase activity allow repeated measurements of individual seedlings over several days to assay dynamic changes in gene expression.
Megan M, Southern, Paul E, Brown, Anthony, Hall   +2 more
openaire   +2 more sources

Flavin binding by bacterial luciferase: Affinity chromatography of bacterial luciferase

Biochemical and Biophysical Research Communications, 1974
Abstract Immobilized FMN covalently attached to Sepharose-6B-hexanoate binds bacterial luciferase. Immobilized flavin is also effective in its reduced form as a substrate in the light emitting reaction catalyzed by luciferase.
C A, Waters, J R, Murphy, J W, Hastings
openaire   +2 more sources

Luciferase reporter assay

2006
This luciferase reporter assay provides a simple and highly sensitive method to determine the responsiveness of the Tet-system. It is quantitative, reproducible and easy to use in DT40 screens with both transient and stable transfections. The reaction catalyzed by firefly luciferase is the oxidation of luciferin in the presence of coenzyme A with ...
Frank R, Wettey, Antony P, Jackson
openaire   +2 more sources

Measurement of Luciferase Rhythms

2014
Firefly luciferase (LUC) is a sensitive and versatile reporter for the analysis of gene expression. Transgenic plants carrying CLOCK GENE promoter:LUC fusions can be assayed with high temporal resolution. LUC measurement is sensitive, noninvasive, and nondestructive and can be readily automated, greatly facilitating genetic studies.
C Robertson, McClung, Qiguang, Xie
openaire   +2 more sources

In Vivo Simultaneous Analysis of Gene Expression by Dual-Color Luciferases in Caenorhabditis elegans

International Journal of Molecular Sciences, 2021
Yoshihiro Ohmiya, Motomichi Doi, Ohmiya Yoshihiro   +2 more
exaly  

Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B1 in Wheat Samples

Analysis & Sensing, 2022
Elena Benito-Peña, Maria C Moreno Bondi, Sapna Deo   +2 more
exaly  

Influence of the C-terminal domain on the bioluminescence activity and color determination in green and red emitting beetle luciferases and luciferase-like enzyme

Photochemical and Photobiological Sciences, 2021
Vadim Viviani, Atilio Tomazini, Mario T Murakami   +2 more
exaly  

Purification of Cypridina luciferase

Journal of Cellular and Comparative Physiology, 1951
W D, McELROY, A M, CHASE
openaire   +2 more sources

De novo design of luciferases using deep learning

Nature, 2023
David Baker, Christoffer Norn, Yakov Kipnis   +2 more
exaly