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The structural origin and biological function of pH-sensitivity in firefly luciferases
Photochemical and Photobiological Sciences, 2008 Firefly luciferases are called pH-sensitive because their bioluminescence spectra display a typical red-shift at acidic pH, higher temperatures, and in the presence of heavy metal cations, whereas other beetle luciferases (click beetles and railroadworms)
Vadim R Viviani +2 more
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Shining Light on the Secreted Luciferases of Marine Copepods: Current Knowledge and Applications
Photochemistry and Photobiology, 2019 Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала.Copepod luciferases -a family of small secretory proteins of 18.4-24.3 kDa, including a signal peptide-are responsible for bright secreted bioluminescence of some marine ...
Svetlana V Markova +2 more
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Luminescence, 2007 Phrixotrix (railroad worm) luciferases produce bioluminescence in the green and red regions of the spectrum, depending on the location of the lanterns, and are the only luciferases naturally producing red bioluminescence.
Vadim R Viviani
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Photochemistry and Photobiology, 2008 Beetle luciferases emit a wide range of bioluminescence colors, ranging from green to red. Firefly luciferases can shift the spectrum to red in response to pH and temperature changes, whereas click beetle and railroadworm luciferases do not. Despite many
Vadim R Viviani +2 more
exaly +2 more sources
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Expert Opinion on Drug Discovery, 2010
By selecting the most appropriate bioluminescence assay, the researcher can study the underlying molecular mechanisms of a physiological system and the effects of a drug throughout the body.This review covers three luciferase assay systems: the multicolor luciferase assay, secreted luciferase assay and imaging luciferase assay. These assays are applied
Yoshihiro, Nakajima, Yoshihiro, Ohmiya
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By selecting the most appropriate bioluminescence assay, the researcher can study the underlying molecular mechanisms of a physiological system and the effects of a drug throughout the body.This review covers three luciferase assay systems: the multicolor luciferase assay, secreted luciferase assay and imaging luciferase assay. These assays are applied
Yoshihiro, Nakajima, Yoshihiro, Ohmiya
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Cold Spring Harbor Protocols, 2010
INTRODUCTIONWhen a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because transfection efficiency in such assays can be low, promoters are commonly fused to heterologous reporter genes that encode enzymes that can be quantified using highly sensitive assays. The reporter protein’
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INTRODUCTIONWhen a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because transfection efficiency in such assays can be low, promoters are commonly fused to heterologous reporter genes that encode enzymes that can be quantified using highly sensitive assays. The reporter protein’
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Structure of bacterial luciferase
Current Opinion in Structural Biology, 1995The generation of light by living organisms such as fireflies, glow-worms, mushrooms, fish, or bacteria growing on decaying materials has been a subject of fascination throughout the ages, partly because it occurs without the need for high temperatures.
T O, Baldwin +6 more
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Journal of Photochemistry and Photobiology B: Biology, 2010
Firefly luciferase (Luc) is the most studied of the luciferase enzymes and the mechanism and kinetics of the reactions catalyzed by this enzyme have been relatively well characterized. Luc catalyzes the bioluminescent reaction involving firefly luciferin (D-LH(2)), adenosine triphosphate (ATP), magnesium ion and molecular oxygen with the formation of ...
João M. M. Leitão +1 more
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Firefly luciferase (Luc) is the most studied of the luciferase enzymes and the mechanism and kinetics of the reactions catalyzed by this enzyme have been relatively well characterized. Luc catalyzes the bioluminescent reaction involving firefly luciferin (D-LH(2)), adenosine triphosphate (ATP), magnesium ion and molecular oxygen with the formation of ...
João M. M. Leitão +1 more
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Crystalline firefly luciferase
Biochimica et Biophysica Acta, 1956Abstract 1. 1. The method for purification and crystallization of firefly luciferase is described. 2. 2. The crystalline enzyme is apparently homogenous as evidenced by ultracentrifugation a and electrophoretic determinations. The molecular weight is probably around 100,000 and the isoelectric point between pH 6.2 and 6.3. 3. 3.
A A, GREEN, W D, MCELROY
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Coelenterazine-dependent luciferases
Biochemistry (Moscow), 2015Bioluminescence is a widespread natural phenomenon. Luminous organisms are found among bacteria, fungi, protozoa, coelenterates, worms, molluscs, insects, and fish. Studies on bioluminescent systems of various organisms have revealed an interesting feature - the mechanisms underlying visible light emission are considerably different in representatives ...
S V, Markova, E S, Vysotski
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