Results 251 to 260 of about 14,115 (284)

Coelenterazine-dependent luciferases

Biochemistry (Moscow), 2015
Bioluminescence is a widespread natural phenomenon. Luminous organisms are found among bacteria, fungi, protozoa, coelenterates, worms, molluscs, insects, and fish. Studies on bioluminescent systems of various organisms have revealed an interesting feature - the mechanisms underlying visible light emission are considerably different in representatives ...
S V, Markova, E S, Vysotski
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Multiplex Hextuple Luciferase Assaying

2022
We recently expanded the commonly used dual luciferase assaying method toward multiplex hextuple luciferase assaying, allowing monitoring the activity of five experimental pathways against one control at the same time. In doing so, while our expanded assay utilizes a total of six orthogonal luciferases instead of two, this assay, conveniently, still ...
Sarrion-Perdigones, Alejandro, Gonzalez, Yezabel, Chang, Lyra, Gallego-Flores, Tatiana, Young, Damian W, Venken, Koen J T   +5 more
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Luciferase Assay

Cold Spring Harbor Protocols, 2010
INTRODUCTIONWhen a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because transfection efficiency in such assays can be low, promoters are commonly fused to heterologous reporter genes that encode enzymes that can be quantified using highly sensitive assays. The reporter protein’
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Luciferase reporter assay

2006
This luciferase reporter assay provides a simple and highly sensitive method to determine the responsiveness of the Tet-system. It is quantitative, reproducible and easy to use in DT40 screens with both transient and stable transfections. The reaction catalyzed by firefly luciferase is the oxidation of luciferin in the presence of coenzyme A with ...
Frank R, Wettey, Antony P, Jackson
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Resveratrol inhibits firefly luciferase

Biochemical and Biophysical Research Communications, 2006
The potential therapeutic value of resveratrol in age-related disease settings including cancer, diabetes, and Alzheimer's has emerged from a rapidly growing body of experimental evidence. Protection from oxidative stress appears to be a common feature of resveratrol that may be mediated through SirT1, though more specific molecular mechanisms by which
Adel, Bakhtiarova, Paul, Taslimi, Stephen J, Elliman, Penelope A, Kosinski, Brian, Hubbard, Michael, Kavana, Daniel M, Kemp   +6 more
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Flavin binding by bacterial luciferase: Affinity chromatography of bacterial luciferase

Biochemical and Biophysical Research Communications, 1974
Abstract Immobilized FMN covalently attached to Sepharose-6B-hexanoate binds bacterial luciferase. Immobilized flavin is also effective in its reduced form as a substrate in the light emitting reaction catalyzed by luciferase.
C A, Waters, J R, Murphy, J W, Hastings
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Structure of bacterial luciferase

Current Opinion in Structural Biology, 1995
The generation of light by living organisms such as fireflies, glow-worms, mushrooms, fish, or bacteria growing on decaying materials has been a subject of fascination throughout the ages, partly because it occurs without the need for high temperatures.
T O, Baldwin, J A, Christopher, F M, Raushel, J F, Sinclair, M M, Ziegler, A J, Fisher, I, Rayment   +6 more
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Luciferases with Tunable Emission Wavelengths

Angewandte Chemie, 2017
AbstractWe introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The luciferases are chimeras of NanoLuc with either SNAP‐tag or HaloTag7. Labeling of the self‐labeling tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore.
Julien Hiblot, Qiuliyang Yu, Marina D.B. Sabbadini, Luc Reymond, Lin Xue, Alberto Schena, Olivier Sallin, Nicholas Hill, Rudolf Griss, Kai Johnsson   +9 more
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Measurement of Luciferase Rhythms

2014
Firefly luciferase (LUC) is a sensitive and versatile reporter for the analysis of gene expression. Transgenic plants carrying CLOCK GENE promoter:LUC fusions can be assayed with high temporal resolution. LUC measurement is sensitive, noninvasive, and nondestructive and can be readily automated, greatly facilitating genetic studies.
C Robertson, McClung, Qiguang, Xie
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Luciferases as Reporter Genes

2006
Luciferase is the ideal reporter gene to provide temporal and spatial information on promoter activity in Arabidopsis and other eukaryotes; the noninvasive detection of luminescence and short half-life of luciferase activity allow repeated measurements of individual seedlings over several days to assay dynamic changes in gene expression.
Megan M, Southern, Paul E, Brown, Anthony, Hall   +2 more
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