Results 251 to 260 of about 11,605 (288)

Mechanical Control of Renilla Luciferase

Journal of the American Chemical Society, 2013
We report experiments where the activity of the enzyme luciferase from Renilla reniformis is controlled through a DNA spring attached to the enzyme. In the wake of previous work on kinases, these results establish that mechanical stress applied through the DNA springs is indeed a general method for the artificial control of enzymes, and for the ...
Chiao-Yu, Tseng, Giovanni, Zocchi
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Avian retroviral expression of luciferase

Virology, 1991
Biologically active replication-competent (subgroups A, B, and C) and replication-defective Rous sarcoma virus-derived vectors containing the cDNA encoding firefly luciferase as a reporter gene were constructed. In these retroviral vectors, luciferase is expressed from a spliced subgenomic mRNA.
E A, Garber, C I, Rosenblum, H T, Chute, L M, Scheidel, H, Chen   +4 more
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The isoelectric point of Cypridina luciferase

Archives of Biochemistry and Biophysics, 1955
Abstract Measurements of the mobility of Cypridina luciferase by means of paper electrophoresis at seven pH values between 2.38 and 6.18 indicate an apparent isoelectric point of 3.28. The reliability of this value is enhanced by the close agreement found between the isoelectric point of bovine plasma albumin determined by paper electrophoresis and
J H, WEIR, F I, TSUJI, A M, CHASE
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Irradiation of Bacterial Luciferase in vivo

Science, 1962
The fading of in vivo luminescence which is characteristically displayed by resting cell suspensions of the luminous bacteria Photobacterium fischeri is partly accounted for by a gradual loss of active luciferase.
C H, BURNS, A D, NEVILL, G H, WHIPPLE
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The construction of a glucose-sensing luciferase

Biosensors and Bioelectronics, 2009
A novel luminescence-based glucose-sensing molecule was created by combining a galactose-/glucose-binding protein (GGBP) with luciferase. The glucose-sensing luciferase (GlcLuc) was constructed using a GGBP fused with a large domain and a small domain of Firefly luciferase (Lluc and Sluc). The luminescence intensity-based analysis with E.
Atsushi, Taneoka, Akane, Sakaguchi-Mikami, Tomohiko, Yamazaki, Wakako, Tsugawa, Koji, Sode   +4 more
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Enzymatic properties of bacterial luciferase

Archives of Biochemistry and Biophysics, 1955
Abstract 1. 1. The present report is concerned with some of the enzymatic properties of highly purified bacterial luciferase, an enzyme which catalyzes a light-emitting reaction in the presence of reduced riboflavin phosphate (FMNH2), a long-chain fatty aldehyde, and oxygen.
W D, McELROY, A A, GREEN
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The Split Luciferase Complementation Assay

2010
A split luciferase complementation assay to study protein-protein interactions within Arabidopsis protoplasts in 96-well plates is described in this protocol. Two proteins of interest, a bait and prey, which are genetically fused to amino- and carboxy-terminal fragments of Renilla luciferase, are transiently expressed in protoplasts.
Naohiro, Kato, Jason, Jones
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An Interphylum Luciferin-Luciferase Reaction

Science, 1960
The light-emitting enzyme-substance systems, heretofore separated from different types of luminescent organisms, exhibit a marked biological specificity and comprise chemically different components. Extracts from a luminescent fish are now found to cross react with a crustacean system; some properties of the new system and implications of the ...
F H, Johnson, Y, Haneda, E H, Sie
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Resveratrol inhibits firefly luciferase

Biochemical and Biophysical Research Communications, 2006
The potential therapeutic value of resveratrol in age-related disease settings including cancer, diabetes, and Alzheimer's has emerged from a rapidly growing body of experimental evidence. Protection from oxidative stress appears to be a common feature of resveratrol that may be mediated through SirT1, though more specific molecular mechanisms by which
Adel, Bakhtiarova, Paul, Taslimi, Stephen J, Elliman, Penelope A, Kosinski, Brian, Hubbard, Michael, Kavana, Daniel M, Kemp   +6 more
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Purification of Cypridina luciferase

Journal of Cellular and Comparative Physiology, 1951
W D, McELROY, A M, CHASE
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