Results 131 to 140 of about 67,421 (349)
Advanced Science, EarlyView.FAK signaling drives chemoresistance and tumor evolution in pancreatic ductal adenocarcinoma. The FAK inhibitor IN10018 restores gemcitabine sensitivity by inducing SLC7A11‐mediated ferroptosis through the PI3K‐Akt pathway and remodeling the tumor microenvironment, reducing mesenchymal components while enhancing CD8+ T‐cell infiltration in preclinical ...
Yingjin Wang +11 more
wiley +1 more source
Application of the luciferin-luciferase enzyme system for determination of adenosine triphosphate (ATP) to studies on the mechanisms of herbicide action [PDF]
The luciferin-luciferase enzyme system for determination of ATP is valuable for studies on the mechanisms of herbicide action. Investigations using this system have shown that certain herbicides may act by interfering with ATP production or by blocking ...
St.john, J. B.
core +1 more source
Light detection instrument Patent [PDF]
, 1970 Detection instrument for light emitted from ATP biochemical ...
Chappelle, E. W., Hoffman, D. G.
core +1 more source
Enhanced Intracellular Stability and Translation Efficiency of mRNA Drugs by a 2‐arm mRNA Platform
Advanced Science, EarlyView.We constructed a 2‐arm mRNA, characterized by a unique topology formed through the dimerization of two mRNA 3’ tails. The 2‐arm mRNA improves 3’ tail stability and resistance to nuclease degradation, resulting in an intracellular half‐life of up to 65 h. This method substantially enhances the translation capacity of mRNA drugs.
Xucong Teng +5 more
wiley +1 more source
Analytical Applications of Bioluminescence and Chemiluminescence [PDF]
Bioluminescence and chemiluminescence studies were used to measure the amount of adenosine triphosphate and therefore the amount of energy available. Firefly luciferase - luciferin enzyme system was emphasized.
Chappelle, E. W., Picciolo, G. L.
core +1 more source
Use of the enzyme hexokinase for the reduction of inherent light levels [PDF]
, 1969 Use of enzyme hexokinase and glucose to reduce inherent light levels of ATP in luciferase ...
Chappelle, E. W.
core +1 more source
Discovery of a Potent Fluorescence Polarization Probe for Identifying USP1 Allosteric Inhibitors
Advanced Science, EarlyView.This study presents the first ubiquitin‐specific protease 1 (USP1) allosteric fluoroprobe and fluorescence polarization assay, enabling the differentiation of allosteric and catalytic site inhibitors. Further, a novel class of tetrahydroisoquinoline‐based USP1 inhibitors is designed, with compound 14a (USP1 IC50 = 29.9 nM) showing strong selectivity ...
Jiawei Cheng +12 more
wiley +1 more source
Advanced Science, EarlyView.A catalytic allosteric DNAzyme assay (SMART) is developed by engineering DNAzyme into a unimolecular biosensor, enabling highly sensitive and multiplex detection of small molecules and nucleic acids. SMART enables extraction‐free, one‐pot, one‐step, isothermal, and cost‐effective detection of biological markers, achieving >95% accuracy for rapid ...
Yanzhe Shen +12 more
wiley +1 more source
Advanced Science, EarlyView.Biological rhythms coordinate physiology, from genes to behavior. Study of circadian rhythms in brain tissue is constrained by limited throughput and spatial and temporal information quality. A new platform for high‐throughput, long‐term multiplexed fluorescent live imaging of circadian rhythms in brain slices is introduced.
Marco Ferrari +3 more
wiley +1 more source
Advanced Science, EarlyView.ABSTRACT Viruses often hijack host developmental programs to promote infection, but the mechanistic links between reproductive regulation and antiviral immunity remain incompletely understood. Here, we identify a virus‐triggered hierarchical degradation cascade that links antiviral immunity and fertility regulation in rice. We show that the rice grassy
Yuansheng Wu +13 more
wiley +1 more source