Bioluminescent monitoring of recombinant lactic acid bacteria and their products
Biotherapeutic strategies to promote health, including the application of engineered microbes to deliver therapeutic molecules, hold strong promise. However, without precision tools to detect therapeutic microbes and their products, we are hampered in ...
In Young Choi+3 more
doaj +1 more source
Synthesis and Characterization of Luminescent Lanthanide Nano-Rings [PDF]
It is not uncommon for inorganic chemistry classes to gloss over the chemistry of the lanthanides. Because most inorganic chemists work closely with the d -electrons of the transition metals, the shielded and inert f -electrons of the lanthanides may at
Bard, Alexander
core +1 more source
Generation of Reporter-Expressing New World Arenaviruses: A Systematic Comparison
Replication-competent reporter-expressing viruses are crucial tools in molecular virology with applications that range from antiviral screening to live-cell imaging of protein spatiotemporal dynamics.
Lucie Fénéant+3 more
doaj +1 more source
Singlet oxygen luminescence detection with a fiber-coupled superconducting nanowire single-photon detector [PDF]
Direct monitoring of singlet oxygen (1O2) luminescence is a particularly challenging infrared photodetection problem. 1O2, an excited state of the oxygen molecule, is a crucial intermediate in many biological processes. We employ a low noise superconducting nanowire single-photon detector to record 1O2 luminescence at 1270 nm wavelength from a model ...
arxiv +1 more source
Interglobular Diffusion of an Energy Donor in Triplet-Triplet Energy Transfer in Proteins
The triplet-triplet energy transfer between polar molecules of luminescent probe (eosin) as an energy donor and nonpolar molecules of energy acceptor (anthracene) is studied. Both the donor and the acceptor are bound to human serum albumin by noncovalent
Andrey G. Melnikov+4 more
doaj +1 more source
Far-red pentamethine cyanine dyes as fluorescent probes for the detection of serum albumins [PDF]
Benzothiazole based cyanine dyes with bridged groups in the pentamethine chain were studied as potential far-red fluorescent probes for protein detection.
D. Aristova+8 more
doaj +1 more source
Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Abbe+295 more
core +2 more sources
Noncovalent Labeling of Biomolecules with Red and Near- Infrared Dyes
Biopolymers such as proteins and nucleic acids can be labeled with a fluorescent marker to allow for their detection. Covalent labeling is achieved by the reaction of an appropriately functionalized dye marker with a reactive group on a biomolecule.
Lucjan Strekowski+3 more
doaj +1 more source
A Modular Cloning Toolkit for the production of recombinant proteins in Leishmania tarentolae
Modular Cloning (MoClo) is based on libraries of standardized genetic parts that can be directionally assembled via Golden Gate cloning in one-pot reactions into transcription units and multigene constructs.
Katrin Hieronimus+20 more
doaj +1 more source
Sodium-dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) can be used to separate proteins based mainly on their size such as in denaturing gels.
Alexander Carreño+11 more
doaj +1 more source