Results 141 to 150 of about 109,979 (261)

Dual‐Component Spidroin‐Based Hydrogels: A Mechanically Tunable Platform for 2D and 3D Cell Culture

Small, EarlyView.
A dual‐component recombinant spider silk hydrogel platform was developed by combining NT2RepCT with a bioactive vitronectin‐functionalized spidroin domain. The hydrogels self‐assemble under physiological conditions, exhibit tunable mechanical properties, and support 2D and 3D cell culture, providing a versatile platform for mechanobiological studies ...
Sanna Korpela, Urmimala Chatterjee, Gabriele Greco, Benjamin Schmuck, Rakesh Kumar, Olga Shilkova, Cecilia Götherström, Anna Rising, Teemu O. Ihalainen   +8 more
wiley   +1 more source

Draft genome sequence of epiphytic <i>Bacillus safensis</i> strain Cap07D, isolated from <i>Chenopodium album</i> L. seeds. [PDF]

Microbiol Resour Announc
Chebotar VK, Antonova VD, Losev MR, Gancheva MS, Kudryavtcev DV, Bortsova OA, Chizhevskaya EP, Pishchik VN.   +7 more
europepmc   +1 more source

Beyond monofunctionality: structural perspectives on phenol oxidase side activity in a catalase

Acta Crystallographica Section D, EarlyView.
Structural and biochemical characterization of the extracellular fungal catalase ClCPO uncovers an unexpected phenol oxidase promiscuity. Crystal structures, substrate docking and electron‐transfer pathway analysis identify ligand‐binding regions and support a working mechanistic model for catalase‐associated phenol oxidase activity.Catalases are ...
Christina Ferousi, Christos Kosinas, Odyssefs Ioannis Pantelakis, Efstratios Nikolaivits, Evangelos Topakas, Maria Dimarogona   +5 more
wiley   +1 more source

An intracellular recombinant single‐chain variable antibody fragment as a new class of phosphodiesterase type 5 inhibitors

British Journal of Pharmacology, EarlyView.
Background and Purpose Cyclic guanosine monophosphate (cGMP) is a ubiquitous second messenger involved in human (patho‐)physiology. Phosphodiesterase 5 (PDE5) is a major cGMP hydrolyzing enzyme in many cell types including vascular smooth muscle cells (VSMCs). Several highly selective PDE5 inhibitors are in clinical use. However, there are currently no
Kürsat Kirkgöz, Sophie Sprenger, Olga Schweigert, Sanika Mohagaonkar, Matti Walsdorff, Tanja Zeller, Filip Berisha, Viacheslav O. Nikolaev, Sergei D. Rybalkin   +8 more
wiley   +1 more source

Genotype‐Dependent Functional and Pharmacological Profiling of Rare GCK Variants Reveals Implications for Precision Management of Monogenic Diabetes

Diabetes, Obesity and Metabolism, EarlyView.
ABSTRACT Aims Rare GCK variants contribute to heterogeneous forms of monogenic diabetes, yet the molecular basis underlying their diverse functional consequences and pharmacological responsiveness remains incompletely understood. As glucokinase (GCK) serves as a central glucose sensor in pancreatic β‐cells and hepatocytes, defining the functional ...
Haoyu Bai, Jie Yu, Qiusha Zhu, Xue Zhou, Ting Xie, Tong Wang, Jing Ren, Chuhan Shao, Qian Zhang, Changhong Li, Xinhua Xiao   +10 more
wiley   +1 more source

A Unique Long-term Monitoring Transient Electromagnetic Dataset. [PDF]

Sci Data
Zamora Luria JC, Christiansen AV.
europepmc   +1 more source

Aptamer‐mediated outer membrane destabilization overcomes low permeability resistance in Pseudomonas aeruginosa

The FEBS Journal, EarlyView.
DNA aptamers, Apt 60 and Apt 173, target the BamA protein to compromise outer membrane integrity in Pseudomonas aeruginosa, making it susceptible to Azithromycin, an antibiotic that is otherwise poorly effective due to the low‐permeability membrane.
Rupany Selvam, Michelle Khai Khun Yap, Rafael Giacomazzi, Sérgio F. Sousa, Hock Siew Tan   +4 more
wiley   +1 more source

Cryo‐EM structure of GH43 β‐Xylosidase from Enterobacter cloacae provides insights into substrate specificity and the role of an auxiliary domain in enzymatic activity

The FEBS Journal, EarlyView.
Xylan, the second most abundant plant cell wall polysaccharide, is degraded by β‐xylanases and β‐xylosidases. Here, we present the 2.65 Å cryo‐EM structure of Enterobacter cloacae β‐xylosidase (EcXyl43, GH43) and the 2.4 Å X‐ray structure of its inactive F507A mutant.
Lorenzo Briganti, Andre Schutzer Godoy, Caio Cesar Mello Capetti, Rafaela Sachetto Fernandes, Vanessa O. A. Pellegrini, Ornella M. Ontañon, Eleonora Campos, Rodrigo V. Portugal, Igor Polikarpov   +8 more
wiley   +1 more source

Maltose aids in the growth of <i>Salmonella</i> Typhimurium in nitrogen-deficient conditions. [PDF]

FEMS Microbes
Parmar K, Kumari Y, Nayak M M, Karmakar K, Bagchi S, Chakravortty D.   +5 more
europepmc   +1 more source

Ligand binding represses bacterial histidine kinase activity by inhibiting its dimerization

The FEBS Journal, EarlyView.
PdtaS is a kinase in the pathogen Mycobacterium tuberculosis that senses copper and nitric oxide and controls virulence gene expression, but how the kinase senses these compounds was unclear. We show that PdtaS is active as a dimer with each monomer phosphorylating the other in trans.
Gaurav D. Sankhe, Jiawei Xing, Xiansha Xiao, John A. Buglino, Huilin Li, Igor B. Zhulin, Michael S. Glickman   +6 more
wiley   +1 more source