Results 161 to 170 of about 109,979 (261)

Ion binding sites and intermediate domain closure induced by D‐serine in the GluD1 ligand‐binding domain

The FEBS Journal, EarlyView.
GluD1 belongs to the ionotropic glutamate receptors and has important functions in the brain and brain diseases. We report the crystal structure of the GluD1 ligand‐binding domain with D‐serine and zinc ions. MD simulations supported promiscuous binding of cations and that chloride ions play a stabilizing role. Pro725 seems to hinder interlobe closure,
Flemming Steen Jørgensen, Emma Grosvald Pedersen, Dilip Narayanan, Karla A. Frydenvang, Jette Sandholm Kastrup   +4 more
wiley   +1 more source

Complete genome sequences of <i>Staphylococcus aureus</i> isolates with a novel sequence type from a patient with hospital-acquired pneumonia. [PDF]

Microbiol Resour Announc
Amusin DB, Nozick SH, Wunderink RG, Ozer EA, Hauser AR.   +4 more
europepmc   +1 more source

Toward a minimal amino acid alphabet for protein design

The FEBS Journal, EarlyView.
Proteins are built from 20 canonical amino acids; however, it is interesting to investigate whether they can also be formed from reduced amino acid alphabets. Using protein design, we designed proteins based on all alphabets comprising 2 to 10 ‘early’ amino acids (those likely to have been present before the origin of life).
Karel Půbal, Kseniia Kushnir, Vojtěch Spiwok, Karolína Loužecká, Vladimír Setnička, Petra Lipovová   +5 more
wiley   +1 more source

Purification of MNase for Use in Ribosomal Profiling of High-Salinity Extremophiles. [PDF]

Bio Protoc
Gregorova P, Isada MF, DiRuggiero J, Sarin PL.   +3 more
europepmc   +1 more source

Dimerization of human PARP15 is required for NAD+ binding and automodification

The FEBS Journal, EarlyView.
This study provides an integrated structural model of human PARP15. Biophysical, enzymatic, and cell‐based analyses identify the dimer as the active oligomeric state capable of binding the substrate NAD+. A co‐crystal structure with a non‐hydrolyzable substrate analog BAD reveals the molecular basis for the dimerization requirement.
Anna Tuovinen, Johan Pääkkönen, Mirko M. Maksimainen, Lea Hirschen, Heli I. Hentilä, Marie Tauscher, Bernhard Lüscher, Carlos Vela‐Rodríguez, Patricia Korn, Lari Lehtiö   +9 more
wiley   +1 more source

Working memory's pointer system is governed by physical objecthood, not spatiotemporal information. [PDF]

Psychon Bull Rev
Balaban H.
europepmc   +1 more source

Single‐Cell Atlas Reveals Neutrophil Heterogeneity in Hypervirulent Klebsiella pneumoniae Infection‐Induced Sepsis

Immunology, EarlyView.
The roles of distinct lung neutrophil compartments involved in hvKp infection remain poorly understood. Here, utilising single‐cell RNA sequencing, we identified four neutrophil subsets in the lungs of hvKp‐infected mice: N1, N2, N3a, and N3b, which were organised along a maturation trajectory.
Xiang Fang, Weizhao Li, Xinyu Zhang, Sohana Akter Mina, Qianling Jiang, Gaochen Zhu, Sheng Chen, Guan Yang   +7 more
wiley   +1 more source

Organic Polyurethane Surface Structures to Manufacture Noneluting Antimicrobial Polyurethanes: A Systematic Review of Literature. [PDF]

Biomed Res Int
Hurck BJ, Vargas Guerrero MG, de Boer C, Dias AA, Arts JJC.   +4 more
europepmc   +1 more source

Topological, Functional, and Phylogenetic Analysis of the Cell Division Site Selection Protein MinJ of Bacillus subtilis

Molecular Microbiology, EarlyView.
MinJ is a nine‐pass transmembrane protein with a cytoplasmic PDZ domain. The PDZ domain, while required for division, is dispensable for localization, and deletion of the transmembrane segments 6 and 7 results in a hyper‐minicell phenotype. ABSTRACT The Min system disassembles FtsZ‐rings after septation in Bacillus subtilis and is localized to the ...
Kehinde O. Adebiyi, Laura C. Lastra, Jane H. Joncha, Sadie B. Ruesewald, Stephen C. Jacobson, Daniel B. Kearns   +5 more
wiley   +1 more source

Optimization of an improved chemically defined medium for high-efficiency expression of glucokinase in Escherichia coli. [PDF]

Microb Cell Fact
Ma Y, Kong J, Wang Z, Chen S, Yang X, Chen M, Wen Z, Xia J.   +7 more
europepmc   +1 more source