Results 181 to 190 of about 109,979 (261)

A Lambda Red Recombinase‐Assisted CRISPR/Cas9 System for Efficient and Precise Genome Editing in Agrobacterium tumefaciens

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Agrobacterium tumefaciens is a powerful delivery tool for plant genetic transformation and a pivotal platform for agricultural biotechnology. However, laboratory strains of Agrobacterium have remained genetically unoptimized for decades, with genome engineering hindered by inefficient, endogenous homologous recombination systems.
Minmin Zheng   +4 more
wiley   +1 more source

Teaching Novices Ultrasound-Assisted Neuraxial Procedures Using Simulation-Based Courses: A Proof of Concept. [PDF]

open access: yesJ Educ Perioper Med
Schutte SS   +10 more
europepmc   +1 more source

Leaf Structure and Hormonal Balance Modulate Host‐Specificity and Pathogenicity of Botrytis cinerea

open access: yesPlant, Cell &Environment, EarlyView.
ABSTRACT Botrytis cinerea, a highly successful necrotrophic fungal plant pathogen, demonstrates a remarkably broad host range, yet the precise factors governing its host specificity and its ability to coexist non‐pathogenically with plants remain unclear. This study systematically assessed host preference in a series of B. cinerea agricultural isolates,
Gautam Anand   +12 more
wiley   +1 more source

Enterobacter supplement and ambient conditions determine the performance of sterile male Mediterranean fruit flies

open access: yesPhysiological Entomology, EarlyView.
Partial replacement of Brewer's yeast (protein source) with inactivated Enterobacter biomass during larval development enhanced mating competitiveness of sterile VIENNA 8 GSS males under outdoor field‐cage conditions. Sterile male mating performance was season‐dependent, with highest competitiveness in spring, intermediate in autumn and the lowest ...
Panagiota Koskinioti   +9 more
wiley   +1 more source

Spectroscopic analysis of bacterial photoreactivation

open access: yesPhotochemistry and Photobiology, Volume 101, Issue 2, Page 494-504, March/April 2025.
This study investigates the effectiveness of fluorescence and Raman spectroscopy in detecting bacterial photoreactivation. Fluorescence spectroscopy shows limitations, because the intense fluorescence of tryptophan and tyrosine masks fluorescence emitted by thymine molecules.
Keyvan Khosh Abady   +4 more
wiley   +1 more source

Microbial Strategies for the Degradation of Spent Lubricating Oil: A Comparative Study of Isolate Bacterium and Consortium Cultures

open access: yesWater and Environment Journal, EarlyView.
This show the biodegradation of spent lubricating oil by Bacillus sp. and a marine bacterial consortium. The consortium showed higher growth, biosurfactant production and oil removal efficiency than the isolated strain, highlighting its potential for bioremediation ABSTRACT Spent automotive lubricating oil contains complex hydrocarbons and heavy metals,
Letícia Carvalho Eustaquio Costa   +5 more
wiley   +1 more source

Ascending infection of Gardnerella spp. and compartmentalized inflammatory response in fetal–maternal interface organ‐on‐chip model

open access: yesThe Journal of Physiology, EarlyView.
Abstract figure legend The fetal–maternal interface organ‐on‐chip was used to investigate the ascending infection of Gardnerella spp. across the decidua (DEC), chorion trophoblast (CTC), amnion mesenchymal (AMC) and amnion epithelial (AEC) compartments over 48 h.
Mariana de Castro Silva   +7 more
wiley   +1 more source

Lipopolysaccharide‐Specific Aptasensor: A Spectroscopic Approach for Identifying Gram‐Negative Bacteria

open access: yesAnalytical Science Advances, Volume 7, Issue 2, December 2026.
We developed a novel aptasensor by integrating an anti‐lipopolysaccharide aptamer with a G‐quadruplex DNAzyme for the detection of Gram‐negative bacteria. This label‐free, spectroscopy‐based sensor detects E. coli with a limit of ∼2.7 × 105 cells/mL, providing a rapid, cost‐effective and specific method for identifying LPS‐containing bacteria ...
Hamidreza Majidifard   +3 more
wiley   +1 more source

Evaluation of tillage and cover cropping systems on the overwintering of angular leaf spot, yield, and quality in dark tobacco

open access: yesCrop, Forage &Turfgrass Management, Volume 12, Issue 2, December 2026.
Abstract Angular leaf spot (ALS; Pseudomonas syringae pv. tabaci) has become an increasingly important disease in dark tobacco [Nicotiana tabacum L.] production since 2015. Current recommendations for controlling ALS include selecting dark tobacco varieties with low ALS susceptibility and using agricultural streptomycin.
Alyssa C. Thelin   +4 more
wiley   +1 more source

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