Results 81 to 90 of about 29,495 (296)

Stereoselective Biotransformation: Transfer of Learning to Advance Drug Metabolism and Biocatalysis

Angewandte Chemie, EarlyView.
Understanding stereoselective biotransformations has implications for predicting drug disposition and response and may also inspire novel biocatalytic and biomimetic strategies to address challenges in metabolite and API synthesis. ABSTRACT Chirality is an important determinant of drug action, as enantiomers can exhibit markedly different ...
Grace A. Okunlola, Godwin A. Aleku
wiley   +2 more sources

Identification and characterization of a critical loop for the high activity of alginate lyase VaAly2 from the PL7_5 subfamily

Frontiers in Microbiology
As the major component in the cell wall of brown algae, alginates are degradable by alginate lyases via β-elimination. Alginate lyases can be categorized into various polysaccharide lyase (PL) families, and PL7 family alginate lyases are the largest ...
Muxuan Du, Muxuan Du, Xue Li, Weipeng Qi, Yingjie Li, Lushan Wang   +5 more
doaj   +1 more source

Alginate Lyases: Sources, Mechanism of Activity and Potencial Application

Squalen, 2013
Alginate lyases are group of enzymes which catalyze depolymerization of alginate into oligosaccharides. Alginate lyase have been widely used in many applications such as in production of bioactive oligosaccharides, control of polysaccharide rheological ...
Subaryono Subaryono, Rosmawaty Peranginangin, Maggy Thenawidjaja Suhartono, Fransiska Rungkat Zakaria   +3 more
doaj   +1 more source

Comparative Characterization of Aspergillus Pectin Lyases by Discriminative Substrate Degradation Profiling

Frontiers in Bioengineering and Biotechnology, 2020
Fungal genomes often contain several copies of genes that encode carbohydrate active enzymes having similar activity. The copies usually have slight sequence variability, and it has been suggested that the multigenecity represents distinct reaction ...
Birgitte Zeuner, Thore Bach Thomsen, Mary Ann Stringer, Kristian B. R. M. Krogh, Anne S. Meyer, Jesper Holck   +5 more
doaj   +1 more source

A dedicated haem lyase is required for the maturation of a novel bacterial cytochrome c with unconventional covalent haem binding [PDF]

, 2007
In bacterial c-type cytochromes, the haem cofactor is covalently attached via two cysteine residues organized in a haem c-binding motif. Here, a novel octa-haem c protein, MccA, is described that contains only seven conventional haem c-binding motifs ...
Allen J.W.A., Allen J.W.A., Allen J.W.A., Allen J.W.A., Beliaev A.S., de Vitry C., Dobbin P.S., Hartshorne S., Kroger A., Lorenzen J.P., Mileni M., Moore G.R., Mowat C.G., Scott R.A., Thony-Meyer L., Walker F.A., Wood P.M., Yang F.   +17 more
core   +1 more source

Engineering a Virus‐Derived X Family DNA Polymerase FvPolX for de novo DNA Synthesis

Advanced Science, EarlyView.
In this study, a Faustovirus‐derived polymerase (FvPolX) is engineered based on structural and sequence analysis of PolX enzymes. The optimal mutants demonstrate substantially enhanced catalytic activity toward dNTPs and 3′‐ONH2‐dNTPs compared to the wild‐type enzyme.
Chengjie Zhang, Lei Du, Shengying Li
wiley   +1 more source

Purification and characterization of alginate lyase from locally isolated marine Pseudomonas stutzeri MSEA04 [PDF]

, 2016
An alginate lyase with high specific enzyme activity was purified from Pseudomonas stutzeri MSEA04, isolated from marine brown algae. The alginate lyase was purified by precipitation with ammonium sulphate, acetone and ethanol individually.
Beltagy, Ehab A., El-Borai, Aliaa, ElAssar, Samy A., Lewiz, Marina   +3 more
core   +1 more source

Kinsenoside Targets IDH1 to Restore Microglial Immune‐Metabolic Homeostasis for Alzheimer's Disease Therapy

Advanced Science, EarlyView.
Dysregulated TCA cycle contributes to Alzheimer's disease (AD) pathogenesis. Here, we show that microglial isocitrate dehydrogenase 1 (IDH1) is a critical driver. Elevated IDH1 disrupts citrate metabolism and mitochondrial function, exacerbating AD pathology.
Qianqian Li, Yajin Liao, Yan‐bo Zhao, Hongxing Wu, Tong Jin, Shuoshuo Li, Yuhan Liu, Peng Li, Songying Ouyang, Zekai Li, YuTing Xia, Qian Hua, Rui‐Yuan Pan, Zengqiang Yuan   +13 more
wiley   +1 more source

Discovery of a novel (R)-selective bacterial hydroxynitrile lyase from Acidobacterium capsulatum

Computational and Structural Biotechnology Journal, 2014
Hydroxynitrile lyases (HNLs) are powerful carbon–carbon bond forming enzymes. The reverse of their natural reaction – the stereoselective addition of hydrogen cyanide (HCN) to carbonyls – yields chiral cyanohydrins, versatile building blocks for the ...
Romana Wiedner, Mandana Gruber-Khadjawi, Helmut Schwab, Kerstin Steiner   +3 more
doaj   +1 more source

A bacterial symbiont in the gill of the marine scallop Argopecten irradians irradians metabolizes dimethylsulfoniopropionate

mLife, 2023
Microbial lysis of dimethylsulfoniopropionate (DMSP) is a key step in marine organic sulfur cycling and has been recently demonstrated to play an important role in mediating interactions between bacteria, algae, and zooplankton.
Yi Shu, Yongming Wang, Zhongcheng Wei, Ning Gao, Shuyan Wang, Chun‐Yang Li, Qiang Xing, Xiaoli Hu, Xiao‐Hua Zhang, Yu‐Zhong Zhang, Weipeng Zhang, Zhenmin Bao, Wei Ding   +12 more
doaj   +1 more source