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Harvesting and cryopreservation of lymphatic endothelial cells for lymphatic tissue engineering

Cryobiology, 2010
In order to provide a suitable source of cells for lymphatic tissue engineering, the present study was designed to investigate techniques for harvesting and cryopreservation of human dermal lymphatic endothelial cells (LECs) in vitro. The LECs were isolated from children's foreskins and then cultured in endothelial growth medium-2 MV (EGM-2-MV) with 5%
Zhaohua, Jiang   +3 more
openaire   +2 more sources

Mechanotransduction in lymphatic endothelial cells.

Lymphology, 2007
Initial lymphatic vessel endothelial cells are connected to the surrounding elastic fibers by fibrillin anchoring filaments that have been hypothesized to favor interstitial fluid drainage in edema pulling apart interendothelial junctions. We hypothesized a biochemical mechanism involving mechanotransduction. This study was designed to verify whether a
ROSSI, A.   +5 more
openaire   +2 more sources

Lymphatic Endothelial Cells, Lymphangiogenesis, and Extracellular Matrix

Lymphatic Research and Biology, 2006
Exciting studies involving the molecular regulation of lymphangiogenesis in lymphatic-associated disorders (e.g., wound healing, lymphedema and tumor metastasis) have focused renewed attention on the intrinsic relationship between lymphatic endothelial cells (LECs) and extracellular matrix (ECM) microenvironment.
openaire   +2 more sources

Histochemical analysis of lymphatic endothelial cells in lymphostasis

Microscopy Research and Technique, 2001
AbstractThe ultrastructure of endothelial cells of intestinal lymphatics and the thoracic duct (TD) and the relation to lymphostasis were examined in rats and monkeys. Localization of 5′‐nucleotidase (5′‐Nase) and endothelial nitric oxide synthase (eNOS) was studied.
R C, Ji, S, Kato
openaire   +2 more sources

Multiparameter Fluorescence-Activated Cell Sorting of Human Lymphatic Endothelial Cells

2022
Multiparameter fluorescence-activated cell sorting (FACS) procedure separates target cells from a total population of cells by using specific signatures that the target cell expresses on their cell surface. For human lymphatic endothelial cells (LECs) this relates to cell surface expression of the CD34LowCD31HighVEGFR-3HighPodoplaninHigh profile that ...
Theresa, Connor   +2 more
openaire   +2 more sources

Transcriptional Control of Lymphatic Endothelial Cell Type Specification

2013
The lymphatic vasculature is the "sewer system" of our body as it plays an important role in transporting tissue fluids and extravasated plasma proteins back to the blood circulation and absorbs lipids from the intestinal tract. Malfunction of the lymphatic vasculature can result in lymphedema and obesity. The lymphatic system is also important for the
Ying, Yang, Guillermo, Oliver
openaire   +2 more sources

Lymphatic Endothelial Cells, Inflammatory Lymphangiogenesis, and Prospective Players

Current Medicinal Chemistry, 2007
De novo lymphangiogenesis influences different pathological courses via modulating tissue fluid homeostasis, macromolecule absorption, and leukocyte transmigration. During the past decade, improved understanding of lymphatic biology, especially VEGF-C/-D/VEGFR-3-mediating lymphangiogenesis has substantially promoted clinical research in lymphatic ...
openaire   +2 more sources

Detection, Isolation and Culture of Lymphatic Endothelial Cells

2004
Lymphatic vessels are essential for the maintenance of normal tissue fluid balance and immune surveillance, but they also provide a pathway for metastasis in many types of cancers (reviewed in Alitalo and Carmeliet 2002). In spite of the importance of lymphatic vessels in medicine, the cell biology of this part of the vascular system has received ...
Mäkinen, T., Alitalo, K.
openaire   +2 more sources

Cytoplasmic bodies in lymphatic endothelial cell.

Lymphology, 1987
Although micropinocytotic vesicles and other large vesicular structures have been observed in lymphatic endothelial cells on electron microscopy, in this study ultrastructure attention was focused on a variety of other membrane-bounded structures in the cytoplasm of these cells in the rabbit heart. The first type was oval or round in transverse section
POGGI, PAOLA   +3 more
openaire   +1 more source

Neurotensin is an anti-thermogenic peptide produced by lymphatic endothelial cells

Cell Metabolism, 2021
Jin Li   +2 more
exaly  

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