Results 31 to 40 of about 50,534 (301)

Untargeted Plant Metabolomics: Evaluation of Lyophilization as a Sample Preparation Technique

Metabolites, 2023
Lyophilization is a common method used for stabilizing biological samples prior to storage or to concentrate extracts. However, it is possible that this process may alter the metabolic composition or lead to the loss of metabolites.
Christina Maisl, Maria Doppler, Bernhard Seidl, Christoph Bueschl, Rainer Schuhmacher   +4 more
doaj   +1 more source

Conservation of Microorganisms of Biotechnological Interest

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology
Thirty strains of microorganisms from the National Collection of Nonpathogenic Microorganisms (NCNM) were used as study objects (actinobacteria, bacteria, and micromycetes).
Tamara SÎRBU, Cristina MOLDOVAN, Valerian SLANINA, Maxim BÎRSA   +3 more
doaj   +1 more source

A strategy to promote the convenient storage and direct use of polyhydroxybutyrate-degrading Bacillus sp. JY14 by lyophilization with protective reagents

Microbial Cell Factories, 2023
Background Bioplastics are attracting considerable attention, owing to the increase in non-degradable waste. Using microorganisms to degrade bioplastics is a promising strategy for reducing non-degradable plastic waste.
Su Hyun Kim, Nara Shin, Suk Jin Oh, Jeong Hyeon Hwang, Hyun Jin Kim, Shashi Kant Bhatia, Jeonghee Yun, Jae-Seok Kim, Yung-Hun Yang   +8 more
doaj   +1 more source

Viability and stability of fungi isolated from lake La izvor after lyophilization [PDF]

, 2022
Lyophilization is one of the safest and most durable methods of preservation of the viability and stability of the biological properties of microorganisms.
ȚURCAN, Olga, MOLDOVAN, Cristina, SÎRBU, Tamara   +2 more
core   +1 more source

An Optimized Freeze-Dry Multimodal Workflow for Sequential Micro-CT Imaging, Histology, and Molecular Profiling: A Use Case in Human Liver Fibrosis. [PDF]

Adv Sci (Weinh)
Optimized freeze‐drying and eosin staining deliver artifact‐free micro‐CT images of liver tissue at near‐histological detail, then hand the same specimen back for sectioning, staining, and sequencing. Fibrotic volume distinguishes normal liver from cirrhosis, while preserved RNA reveals inflammatory gene upregulation—one biopsy, three dimensions, and a
Skok K, Činč Ćurić L, Vajda J, Schauer S, Wagner M, Stauber R, Lackner C, Kashofer K, Plass M, Blanco GR, Wanless IR, Maver U, Panzitt K, Vihar B.   +13 more
europepmc   +2 more sources

Development of Lyophilized Gemini Surfactant-Based Gene Delivery Systems: Influence of Lyophilization on the Structure, Activity and Stability of the Lipoplexes

Journal of Pharmacy & Pharmaceutical Sciences, 2012
Purpose. Cationic gemini surfactants have been studied as non-viral vectors for gene therapy. Clinical applications of cationic lipid/DNA lipoplexes are restricted by their instability in aqueous formulations.
Waleed Mohammed-Saeid, Deborah Michel, Anas El-Aneed, Ronald E Verrall, Nicholas H Low, Ildiko Badea   +5 more
doaj   +1 more source

POC-CCA result before and after lyophilization step for 10 times concentration of urine.

, 2016
(A) Diagnosis of the same individual with negative result before urine lyophilization and positive result after urine lyophilization. (B) Diagnosis of the same individual with trace result before urine lyophilization and positive result after urine ...
Nathalie Bonatti Franco Almeida (3165459), Edward Oliveira (112010), Naftale Katz (227432), Rafaella Fortini Queiroz Grenfell (518271), Áureo Almeida (3165465), Nídia Francisca de Figueiredo Carneiro (3165468), Liliane Maria Vidal Siqueira (3165462), Paulo Marcos Zech Coelho (305742)   +7 more
core   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Modernizing lyophilization of pharmaceuticals in unit doses via continuous manufacturing [PDF]

, 2019
• Continuous lyophilization improves both intra-vial and vial-to-vial uniformity • Continuous lyophilization reduces processing time by 6 times • Continuous lyophilization better reserves enzymes` activity • Continuous lyophilization reduces equipment
roberto Pisano
core  

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

FEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil, Jeffrey A. Hawkes, Cecilia Blikstad, Pia Lindberg   +3 more
wiley   +1 more source