Results 31 to 40 of about 20,636 (170)

Recombinant Monoclonal Antibodies for Detecting the Tubulin Post‐Translational Modifications Glutamylation and Lysine‐40 Acetylation

Cytoskeleton, EarlyView.
ABSTRACT Post‐translational modifications (PTMs) to tubulin subunits in microtubule filaments are thought to comprise a component of the tubulin code that specifies microtubule functions in cell physiology and animal development. Acetylation of Lysine‐40 (K40) on α‐tubulin (αTub‐K40ac) and glutamylation of both α‐ and β‐tubulin are two tubulin PTMs of ...
Lynne Blasius, Ezekiel C. Thomas, Erik H. Collet, Erin Jenson, Chad G. Pearson, Ryoma Ohi, Kristen J. Verhey   +6 more
wiley   +1 more source

Substitution of lysine 213 with arginine in penicillin-binding protein 5 of Escherichia coli abolishes D-alanine carboxypeptidase activity without affecting penicillin binding.

Journal of Biological Chemistry, 1992
All penicillin-binding proteins (PBPs) contain a conserved box of homology in the carboxyl-terminal half of their primary sequence that can be Lys-Thr-Gly, Lys-Ser-Gly, or His-Thr-Gly. Site-saturation mutagenesis was used to address the role of the lysine residue at this position (Lys213) in Escherichia coli PBP 5, a D-alanine carboxypeptidase enzyme ...
K T, Malhotra, R A, Nicholas
openaire   +2 more sources

A personal 360° view of applications of ‘biomimetic’ molecular recognition

Journal of Chemical Technology &Biotechnology, EarlyView.
Abstract Molecular recognition between biological molecules has formed the basis for innumerable applications in biotechnology for the last seven decades or so. Techniques such as affinity chromatography, solid‐phase and aqueous two‐phase extraction, affinity precipitation, biomimetic catalytic systems, biosensors and molecular imprinting all exploit ...
Christopher R Lowe
wiley   +1 more source

The Effects of Dried Apple Pomace on Fermentation Quality and Proteolysis of Alfalfa Silages

Agronomy
This work aimed to evaluate the effects of dried apple pomace (DAP) on the fermentation characteristics and proteolysis of alfalfa silages. The alfalfa was ensiled with (1) no additives (control), (2) 5% DAP, (3) 10% DAP and (4) 15% DAP based on fresh ...
Tongtong Dai, Jiangyu Long, Guanjun Zhang, Xianjun Yuan, Zhihao Dong   +4 more
doaj   +1 more source

Cyanopeptide Mixtures Induce Variable Synergistic and Antagonistic Effects Across Diverse Human Cell Lines

Environmental Toxicology, EarlyView.
ABSTRACT Cyanobacterial harmful algal blooms (cyanoHABs) threaten human, animal, and ecosystem health and safety through production of toxic secondary metabolites. Microcystis, a cosmopolitan bloom‐forming cyanobacterial genus, is well‐known for producing hepatotoxic microcystins (MCs), but it can produce many other bioactive cyanopeptides, such as ...
Lauren N. Hart, Katherine L. Lev, Sierra Hefferan, Sanduni H. Premathilaka, Sharmila I. Thenuwara, Dragan Isailovic, David H. Sherman, Gregory J. Dick   +7 more
wiley   +1 more source

High-resolution structure of the M14-type cytosolic carboxypeptidase from Burkholderia cenocepacia refined exploiting PDB_REDO strategies [PDF]

, 2014
A potential cytosolic metallocarboxypeptidase from Burk­holderia cenocepacia has been crystallized and a synchrotron-radiation microfocus beamline allowed the acquisition of diffraction data to 1.9 Å resolution.
An, Bond, Chen, Cho, Christianson, Christianson, Cruickshank, Dawson, Eadsforth, Emsley, Engh, Evans, Faming, Fauman, Gardell, Gomis-Rüth, Hunter, Joosten, Joosten, Kabsch, Kalinina, Kim, Kimura, Krissinel, Krissinel, Leslie, Mangani, McCoy, Moynie, Murshudov, Otero, Painter, Rawlings, Robbie P. Joosten, Rodriguez de la Vega, Rogowski, Sahab, Schormann, Sievers, Thomas C. Eadsforth, Vadim Rimsa, William N. Hunter, Winn, Winstanley   +43 more
core   +1 more source

Degradomics for large‐scale mechanistic insights on proteases and proteolysis in human health

The FEBS Journal, EarlyView.
Proteolysis has an important role in human disease but remains relatively unexplored. Degradomics, the uncovering of proteolysis in tissues, cells, and proteins, uses mass spectrometry‐based terminomics to identify protein termini occurring therein (forward degradomics) and to define the actions of proteases (reverse degradomics).
Daniel R. Martin, Sumit Bhutada, Suneel S. Apte   +2 more
wiley   +1 more source

Plant Peptides on the Rise: From Historical Insight to Future Applications

Plant Biotechnology Journal, EarlyView.
ABSTRACT Plant peptides constitute a rapidly expanding class of signalling molecules essential to plant physiology, mediating key processes such as development, stress adaptation, and immune responses. This review traces the history of plant peptide research, from the seminal discovery of systemin to the recent identification of non‐canonical peptides (
Shunxi Wang, Jinghua Zhang, Minghui Chen, Bokai Zhang, Huina Zhao, Liuji Wu   +5 more
wiley   +1 more source

A comparative study of an aminopeptidase from lactic acid bacteria: a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University [PDF]

, 1992
Aminopeptidase enzymes from the proteolytic systems of S.salivarius subsp.thermophilus Lactococcus lactis subsp.cremoris and Lactococcus lactis subsp.lactis have been investigated.
Midwinter, Robyn Gillian
core  

Hypoxia and the cytoskeleton

The Journal of Physiology, EarlyView.
Abstract figure legend Schematic outlining the activation of hypoxia‐sensitive pathways, the influence of hypoxia and associated pathways on the cytoskeleton, and the impact of these on disease progression. Abstract A highly‐regulated and dynamic cytoskeleton is vital for functional cellular physiology and the maintenance of homeostasis.
Darragh Flood, Cormac T. Taylor
wiley   +1 more source