Results 101 to 110 of about 3,637,202 (294)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Cognitive flexibility predicts early reading skills [PDF]

, 2014
An important aspect of learning to read is efficiency in accessing different kinds of linguistic information (orthographic, phonological, and semantic) about written words.
Blaye, Agnès, Colé, Pascale, Agnès eBlaye, Pascale eColé, Agnès Blaye, Pascale Colé, Duncan, Lynne G.; id_orcid, Duncan, Lynne G., Lynne G. Duncan   +8 more
core   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Tracking Eye Movements in Sight Translation – the comprehension process in interpreting [PDF]

, 2011
[[abstract]]While the three components of interpreting have been identified as comprehension, reformulation, and production, the process of how these components occur has remained relatively unexplored.
黃致潔
core  

Skim reading: an adaptive strategy for reading on the web

, 2014
It has been suggested that readers spend a great deal of time skim reading on the Web and that if readers skim read they reduce their comprehension of what they have read.
Mark J. Weal, Fitzsimmons, Gemma, Denis Drieghe, Drieghe, Denis, Gemma Fitzsimmons, Weal, Mark   +5 more
core   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Unveiling the cognitive features of L2 Chinese reading: Machine learning reveals subskill-specific and general eye-movement patterns

Acta Psychologica
Traditional reading assessments rely heavily on offline summative outcomes, obscuring the implicit cognitive dynamics of online processing. To render these latent cognitive dynamics empirically tractable, this study employs a data-driven machine learning
Siyuan Xie, Cheng Yang
doaj   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription

FEBS Open Bio, EarlyView.
In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang, Mingqiang Deng, Dongmei He, Diqin Lai, Dongjun Liu, Dengyong Zhu, Zhenhua Zhang, Peng Tang, Chuanman Zhou, Menghui Yin, Xichen Bao   +10 more
wiley   +1 more source

Brainstorming Activities for "Reading Comprehension" of Materials on Japanese Society and Culture [PDF]

, 2013
Simply put, brainstorming is an activity that enables a small group of individuals, interacting spontaneously, to come up with an assortment of ideas in order to achieve a certain purpose. In this article, I will focus on the brainstorming activities for
Midori INDOH, 380
core