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Madin-Darby canine kidney cells

Pfl�gers Archiv European Journal of Physiology, 1990
Vectorial transport of salt and water in the Madin-Darby canine kidney (MDCK) cell line is indicated by the formation of domes when a monolayer is grown on an impermeable support. We investigated aldosterone-induced dome formation and evaluated the dome as an experimental model.
H, Oberleithner, U, Vogel, U, Kersting
  +7 more sources

Transport of choline by Madin-Darby canine kidney cells

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1993
Choline is an essential precursor for the synthesis of phosphatidylcholine, the most abundant phospholipid classes in renal cells, as well as for the synthesis of the osmolyte glycerophosphorylcholine. The characteristics of choline uptake in the renal epithelial cell line MDCK were investigated.
P, Zlatkine   +4 more
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Cyclooxygenase expression in canine platelets and Madin-Darby canine kidney cells

American Journal of Veterinary Research, 2000
Abstract Objective—To examine cyclooxygenase (COX) expression in canine platelets and Madin-Darby canine kidney (MDCK) cells in culture. Sample Population—Canine platelets and MDCK cells. Procedure—Total RNA was recovered from isolated canine platelets and MDCK cells.
P A, Kay-Mugford   +3 more
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Alkaline stress transforms Madin-Darby canine kidney cells

Pfl�gers Archiv European Journal of Physiology, 1991
Similar to growth factors aldosterone stimulates Na+/H+ exchange in renal target cells leading to cytoplasmic alkalinization. An alkaline intracellular pH reduces the H+ bonds between repressor proteins and DNA leading to the destabilization of the nuclear chromatin.
H, Oberleithner   +2 more
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Effects of foscarnet on the cell kinetics of Madin-Darby canine kidney cells

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1983
The antiherpes compound, foscarnet (trisodium phosphonoformate), showed concentration-dependent effects on the cell kinetics of Madin-Darby canine kidney cells. At 1 mM, only minor effects could be seen on cell proliferation and cell cycle distribution, as measured by flow cytometry DNA analysis.
K, Stenberg, S, Skog, B, Tribukait
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Ultrastructural study of Crytosporidium development in Madin-Darby canine kidney cells

Veterinary Parasitology, 1993
Transmission electron microscope studies have been made into phases of the life cycle of a bovine isolate of Cryptosporidium cultured in vitro on Madin-Darby canine kidney cells. The cytoplasm of parasitized cells was noticeably altered, including marked vacuolization and the appearance of membrane structures close to the developing parasites.
M J, Rosales, C, Mascaro, A, Osuna
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Apical uptake of radiolabelled ochratoxin A into Madin–Darby canine kidney cells

Toxicology, 1998
Uptake of ochratoxin A (OTA) across the apical cell membrane of collecting duct cells is the first step in reabsorption and partly mediated by proton-dipeptide cotransport. As the remaining part of apical OTA uptake remained unclear, we studied the characteristics of apical uptake of tritium-labelled OTA (3H-OTA) in MDCK-C11 cells in detail.
G, Schwerdt   +3 more
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Secretion of proteases in serglycin transfected Madin–Darby canine kidney cells

The FEBS Journal, 2006
Madin–Darby canine kidney (MDCK) cells, which do not normally express the proteoglycan (PG) serglycin, were stably transfected with cDNA for human serglycin fused to a polyhistidine tag (His‐tag). Clones with different levels of serglycin mRNA expression were generated.
Lillian, Zernichow   +4 more
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Apparent chloride conductance of subconfluent Madin Darby canine kidney cells

Pfl�gers Archiv European Journal of Physiology, 1986
In incompletely confluent Madin Darby canine kidney (MDCK)-cells continuous measurements of the potential difference across the cell membrane (PD) were made with conventional microelectrodes during rapid changes of extracellular chloride concentration. During control conditions mimicking in vivo situation, PD averages -50.3 +/- 0.7 mV.
F, Lang, M, Defregger, M, Paulmichl
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Effects of epinephrine on electrical properties of Madin-Darby canine kidney cells

Pflügers Archiv European Journal of Physiology, 1986
The present study has been performed, to test for the influence of epinephrine on the potential difference across the cell membrane (PD) of Madin-Darby canine kidney (MDCK) cells. Under control conditions, mimicking the in vivo situation, PD averages - 53.3 +/- 0.9 mV (n = 37). Increasing extracellular potassium concentration from 5.4 to 10 and 20 mmol/
M, Paulmichl, M, Defregger, F, Lang
openaire   +2 more sources

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