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The effect of hypoosmolarity on the electrical properties of Madin Darby canine kidney cells
Pfl�gers Archiv European Journal of Physiology, 1989The present study has been performed to test for the effect of hypotonic extracellular fluid on the electrical properties of Madin Darby canine kidney (MDCK)-cells. The volume of suspended MDCK-cells is 1,892 +/- 16 fl (n = 8) in isotonic (298.7 mosmol/l) extracellular fluid.
M, Paulmichl +3 more
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Electrical properties of Madin-Darby-canine-kidney cells
Pfl�gers Archiv European Journal of Physiology, 1986In incompletely confluent Madin Darby canine kidney cells continuous measurements of the potential difference across the cell membrane (PD) were made with conventional microelectrodes during rapid changes of extracellular sodium and/or calcium concentration. During control conditions PD averages -50.6 +/- 0.7 mV.
M, Paulmichl, F, Friedrich, F, Lang
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Ca2+ Mobilization Evoked by Chloroform in Madin-Darby Canine Kidney Cells
The Journal of Pharmacology and Experimental Therapeutics, 2000The effect of chloroform on Ca(2+) mobilization in Madin-Darby canine kidney cells was examined by using Fura-2 as a Ca(2+) probe. Chloroform (24-248 mM) concentration dependently increased intracellular Ca(2+) concentration ([Ca(2+)](i)). Ca(2+) removal inhibited the Ca(2+) signals evoked by 93 to 248 mM chloroform by reducing both the initial rise ...
C R, Jan, L W, Chen, M W, Lin
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[31] Characterization of hormone-sensitive Madin—Darby canine kidney cells
1985Cultured cells, especially the established lines, provide a continuous and homogeneous system for studying hormone action in intact cells. The cell line known as Madin–Darby canine kidney (MDCK) cells, derived from normal dog kidney more than 20 years ago, is ideal for this type of research, because it retains differentiated functions in culture and ...
Lin, MC, Beckner, SK, Darfler, FJ
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Ca2+ mobilization induced by ?-hexachlorocyclohexane in Madin Darby canine kidney cells
Drug Development Research, 2000The effect of the pesticide δ-hexachlorocyclohexane (δ-HCH) were examined on Ca 2+ signaling in Madin Darby canine kidney (MDCK) using fura-2 as a Ca 2+ probe. δ-HCH at concentrations of 5-200 mM increased intracellular free Ca 2+ concentration ([Ca 2+ ] i ) concentration-dependently. The [Ca 2+ ] i increase comprised an immediate rise followed by
Chung‐Ren Jan +5 more
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Sample Preparation of Culture Medium from Madin-Darby Canine Kidney Cells
2008A reproducible, standardized and simple sample preparation methodology is the key to successful two-dimensional gel electrophoresis (2-DE). This chapter describes step-by-step the sample preparation of culture medium from Madin-Darby canine kidney (MDCK) cells. Tips and tricks are given to circumvent possible pitfalls.
Ambort, Daniel +2 more
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Electrical properties of Madin-Darby canine kidney cells.
Federation proceedings, 1984Madin-Darby canine kidney (MDCK) cells form monolayers with properties resembling natural transporting epithelia. In this paper we first summarize briefly information on the electrical properties of the whole monolayer and on the paracellular permeation route, and then concentrate on the electrical properties of the cells themselves as studied with ...
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Stimulation of deacylation in Madin-Darby canine kidney cells
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1983Gary A Beaudry +3 more
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