Cyclooxygenase expression in canine platelets and Madin-Darby canine kidney cells
American Journal of Veterinary Research, 2000Abstract Objective—To examine cyclooxygenase (COX) expression in canine platelets and Madin-Darby canine kidney (MDCK) cells in culture. Sample Population—Canine platelets and MDCK cells. Procedure—Total RNA was recovered from isolated canine platelets and MDCK cells.
P A, Kay-Mugford +3 more
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Apparent chloride conductance of subconfluent Madin Darby canine kidney cells
Pfl�gers Archiv European Journal of Physiology, 1986In incompletely confluent Madin Darby canine kidney (MDCK)-cells continuous measurements of the potential difference across the cell membrane (PD) were made with conventional microelectrodes during rapid changes of extracellular chloride concentration. During control conditions mimicking in vivo situation, PD averages -50.3 +/- 0.7 mV.
F, Lang, M, Defregger, M, Paulmichl
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Zinc alters actin filaments in Madin-Darby canine kidney cells
Toxicology and Applied Pharmacology, 1992Exposure of semiconfluent cultures of Madin-Darby canine kidney cells to 10 microM zinc leads to a change in the organization of the actin filament system. Most of the stress fibers at the basal end of the cell are lost and the actin associated with the lateral membrane and junctional regions appears to retract into the cytoplasm.
J W, Mills +3 more
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Bradykinin-evoked Ca2+ mobilization in Madin Darby canine kidney cells
European Journal of Pharmacology, 1998We studied the mechanisms underlying the bradykinin-evoked changes in intracellular calcium concentration ([Ca2+]i) in Madin Darby canine kidney (MDCK) cells. Bradykinin evoked a [Ca2+]i transient in a dose-dependent manner, measured by fura-2 fluorimetry and digital video imaging.
C R, Jan, C M, Ho, S N, Wu, C J, Tseng
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Fluoxetine-induced Ca 2+ signals in Madin-Darby canine kidney cells
Naunyn-Schmiedeberg's Archives of Pharmacology, 2001The effect of fluoxetine on Ca2+ signaling in Madin-Darby canine kidney (MDCK) cells was investigated by using fura-2 as a Ca2+ probe. Fluoxetine increased [Ca2+]i concentration-dependently between 5 microM and 200 microM with an EC50 value of 40 microM. The response was reduced by external Ca2+ removal by 30%40%.
K Y, Tang +6 more
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[31] Characterization of hormone-sensitive Madin—Darby canine kidney cells
1985Cultured cells, especially the established lines, provide a continuous and homogeneous system for studying hormone action in intact cells. The cell line known as Madin–Darby canine kidney (MDCK) cells, derived from normal dog kidney more than 20 years ago, is ideal for this type of research, because it retains differentiated functions in culture and ...
Lin, MC, Beckner, SK, Darfler, FJ
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Secretion of proteases in serglycin transfected Madin–Darby canine kidney cells
The FEBS Journal, 2006Madin–Darby canine kidney (MDCK) cells, which do not normally express the proteoglycan (PG) serglycin, were stably transfected with cDNA for human serglycin fused to a polyhistidine tag (His‐tag). Clones with different levels of serglycin mRNA expression were generated.
Lillian, Zernichow +4 more
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Purinoceptor-stimulated phosphoinositide hydrolysis in Madin-Darby canine kidney (MDCK) cells
Naunyn-Schmiedeberg's Archives of Pharmacology, 1997Extracellular nucleotides, acting through P2-purinoceptors, have been implicated in the regulation of ion transport in epithelia, including Madin-Darby canine kidney (MDCK) cells. In this study, experiments were conducted to characterize the P2-purinoceptor subtype on MDCK cells responsible for stimulating inositol phosphate (IP) accumulation using a ...
C M, Yang +7 more
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CP55,940 increases intracellular Ca2+ levels in Madin-Darby canine kidney cells
Life Sciences, 2001The effect of CP55,940, a presumed CB1/CB2 cannabinoid receptor agonist, on intracellular free Ca2+ levels ([Ca2+]i) in Madin-Darby canine kidney cells was examined by using the fluorescent dye fura-2 as a Ca2+ indicator. CP55,940 (2-50 microM) increased [Ca2+]i concentration-dependently with an EC50 of 8 microM. The [Ca2+]i signal comprised an initial
K J, Chou +8 more
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Transport of poly amidoamine dendrimers across Madin–Darby canine kidney cells
International Journal of Pharmaceutics, 2001The objective of this study was to determine the permeability of a series of poly amidoamine (PAMAM) dendrimers of generations 0-4 (G0-G4) across MDCK (Madin-Darby Canine Kidney) cell line. PAMAM dendrimers with incremental increase in size and molecular weight were labeled by fluorescein isothiocyanate (FITC) and the least polydisperse fractions were ...
F, Tajarobi +4 more
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