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Madin-Darby canine kidney cells
Pfl�gers Archiv European Journal of Physiology, 1990Functionally and morphologically, Madin-Darby canine kidney (MDCK) cells resemble intercalated cells of urinary epithelia. Experiments were performed on domes of confluent MDCK monolayers to test for apical H+ secretion. Apical application of 10(-3) mol/l amiloride or of Na(+)-free solution significantly reduced the limiting pH gradient across the dome
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Alkaline stress transforms Madin-Darby canine kidney cells
Pfl�gers Archiv European Journal of Physiology, 1991Similar to growth factors aldosterone stimulates Na+/H+ exchange in renal target cells leading to cytoplasmic alkalinization. An alkaline intracellular pH reduces the H+ bonds between repressor proteins and DNA leading to the destabilization of the nuclear chromatin.
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Transport of choline by Madin-Darby canine kidney cells
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1993Choline is an essential precursor for the synthesis of phosphatidylcholine, the most abundant phospholipid classes in renal cells, as well as for the synthesis of the osmolyte glycerophosphorylcholine. The characteristics of choline uptake in the renal epithelial cell line MDCK were investigated.
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