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Effects of serotonin on electrical properties of Madin-Darby canine kidney cells
Pfl�gers Archiv European Journal of Physiology, 1988The present study has been performed to test for the influence of serotonin on the potential difference across the cell membrane (PD) of Madin-Darby canine kidney (MDCK)-cells. Under control conditions PD averages -48.6 +/- 0.6 mV (n = 98). Increasing extracellular potassium concentration from 5.4 to 10 and 20 mmol/l depolarizes the cell membrane by +6.
M, Paulmichl +4 more
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Effect of sevoflurane on Ca2+ mobilization in Madin–Darby canine kidney cells
Biochemical Pharmacology, 2000We investigated the effect of the volatile anesthetic sevoflurane on Ca2+ signaling in Madin-Darby canine kidney (MDCK) cells by using the fluorescent dye fura-2/AM (1-[2-(5-carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2'-amino-5'-methylphenoxy)-ethane-N,N,N,N-tetraacetic acid pentaace-toxymethyl ester) as the Ca2+ indicator. At a concentration of 0.
C R, Jan, K Y, Wang, C J, Tseng
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Calcium Entry Stimulated by Swelling of Madin-Darby Canine Kidney Cells
Nephron, 2008Cell swelling in Madin-Darby canine kidney (MDCK) cells by reduction of extracellular osmolarity (omission of 70 and 150 mmol/l mannitol, respectively) leads to the activation of anion channels and Ca2+ sensitive K+ channels. The K+ channel activation leads to an initial transient hyperpolarization of the cell membrane potential (PD) followed by a ...
E, Wöll +4 more
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Zinc alters actin filaments in Madin-Darby canine kidney cells
Toxicology and Applied Pharmacology, 1992Exposure of semiconfluent cultures of Madin-Darby canine kidney cells to 10 microM zinc leads to a change in the organization of the actin filament system. Most of the stress fibers at the basal end of the cell are lost and the actin associated with the lateral membrane and junctional regions appears to retract into the cytoplasm.
J W, Mills +3 more
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Electrical properties of Madin-Darby canine kidney cells
Pfl�gers Archiv European Journal of Physiology, 1985To gain some insight into electrogenic transport processes across the plasma membrane of Madin-Darby canine kidney (MDCK)-cells, continuous measurements of the potential difference across the plasma measurements of the potential difference across the plasma membrane (PD) were made during step changes of extracellular ion composition as well as ...
M, Paulmichl, G, Gstraunthaler, F, Lang
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Bradykinin-evoked Ca2+ mobilization in Madin Darby canine kidney cells
European Journal of Pharmacology, 1998We studied the mechanisms underlying the bradykinin-evoked changes in intracellular calcium concentration ([Ca2+]i) in Madin Darby canine kidney (MDCK) cells. Bradykinin evoked a [Ca2+]i transient in a dose-dependent manner, measured by fura-2 fluorimetry and digital video imaging.
C R, Jan, C M, Ho, S N, Wu, C J, Tseng
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Effect of triethyltin on Ca2+ movement in Madin–Darby canine kidney cells
Human & Experimental Toxicology, 2002The effects of the environmental toxicant, triethyltin, on Ca 2 + mobilization in Madin–Darby canine kidney (MDCK) cells have been examined. Triethyltin induced an increase in cytosolic free Ca 2 + levels ([Ca 2 +]
B P, Jiann +5 more
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The action of epinephrine on Madin-Darby canine kidney cells.
Federation proceedings, 1984We have used cultured monolayers of Madin-Darby canine kidney (MDCK) cells, which form epithelial layers of high transepithelial resistance, grown on Millipore filters, for transport studies. In the absence of hormones net ion transport is of small magnitude and is consistent with a net absorptive flow (apical to basal) of Na+.
N L, Simmons, C D, Brown, E L, Rugg
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Effect of trifluoperazine on renal epitheloid madin‐darby canine kidney cells
Journal of Cellular Physiology, 1991AbstractFollowing exposure to a number of hormones, the cell membrane in Madin‐Darby Canine Kidney (MDCK) cells is hyperpolarized by increase of intracellular calcium activity. The present study has been performed to elucidate the possible role of calmodulin in the regulation of intracellular calcium activity and cell membrane potential.
M, Paulmichl +4 more
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Effect of chlorhexidine on Madin Darby canine kidney cells.
Molecular toxicology, 1989Sheets of MDCK cells were severely damaged by the commonly used antiseptic, chlorhexidine. Damage was indicated by changes in the characteristic blister formation normally observed in sheets of these cells growing on the base of culture flasks. Results are given for reduction in blister number with increasing chlorhexidine concentration.
Z, Jabar, M, Dawson
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