Results 81 to 90 of about 32,238 (217)

Attenuation of Influenza a Virus into Live Vaccines Through C‐End Degrons

Advanced Science, Volume 13, Issue 19, 2 April 2026.
Naturally occurring C‐end degrons are exploited to design proteolysis‐targeting live attenuated influenza vaccines. C‐end degron tagging of viral M1 protein promotes proteasome‐dependent degradation, resulting in robust attenuation in host cells while permitting scalable production in engineered cells.
Ping Wang, Le Li, Yunfang Chen, Le Tong, Zhen Li, Rong Yu, Quan Shen, Qikai Wang, Jihuan Hou, Qisi Zhang, Xu Si, Ning Wang, Demin Zhou, Wen‐xia Tian, Longlong Si   +14 more
wiley   +1 more source

Chlorpromazine Induces Basolateral Aquaporin-2 Accumulation via F-Actin Depolymerization and Blockade of Endocytosis in Renal Epithelial Cells

Cells, 2020
We previously showed that in polarized Madin–Darby canine kidney (MDCK) cells, aquaporin-2 (AQP2) is continuously targeted to the basolateral plasma membrane from which it is rapidly retrieved by clathrin-mediated endocytosis.
Richard Bouley, Naofumi Yui, Abby Terlouw, Pui W. Cheung, Dennis Brown   +4 more
doaj   +1 more source

Monoclonal Antibodies 13A4 and AC133 Do Not Recognize the Canine Ortholog of Mouse and Human Stem Cell Antigen Prominin-1 (CD133). [PDF]

PLoS ONE, 2016
The pentaspan membrane glycoprotein prominin-1 (CD133) is widely used in medicine as a cell surface marker of stem and cancer stem cells. It has opened new avenues in stem cell-based regenerative therapy and oncology.
Kristina Thamm, Sylvi Graupner, Carsten Werner, Wieland B Huttner, Denis Corbeil   +4 more
doaj   +1 more source

Calcium Shock Enables Efficient and Programmable Particle Delivery for Genome Editing Applications

Advanced Science, Volume 13, Issue 21, 13 April 2026.
Classical transfection and transduction are inefficient, particularly with confluent cells and organoids, and lack cell type‐specific programmability. This study presents calcium shock (CaSh), a method that dramatically improves particle delivery into single cells, colonies, and organoids.
Nicole Vo, Lorena de Oñate, Maximillian Frank, Xian Hu, Sophie M. Blackburn, Dana V. Foss, Jasmine Villegas, Kunica Asija, Dirk Hockemeyer, Hongxia Fu, Raymond J. Monnat, Ross C. Wilson, Benjamin S. Freedman   +12 more
wiley   +1 more source

Toward Dynamic Liquid Cell Scaffold: Photoreversible Ion Gels Exhibiting Light‐Induced Sol‐Gel Transitions

Macromolecular Rapid Communications, Volume 47, Issue 7, 3 April 2026.
Light‐programmable, non‐cytotoxic ion gels based on ABC triblock copolymers undergo photoreversible sol‐gel transitions, crossing the rheological boundary (tan δ ∼ 1). UV–vis light irradiation modulates the lifetime of reversible junctions, switching the scaffold between liquid‐like and solid (gel)‐like states.
Aya Saruwatari, Yuji Kamiyama, Ryota Tamate, Jun Nakanishi, Takeshi Ueki   +4 more
wiley   +1 more source

Hyperosmolality regulates endothelin release by Madin-Darby canine kidney cells. [PDF]

Journal of the American Society of Nephrology, 1993
ET release by the renal epithelial Madin-Darby canine kidney (MDCK) cell line was investigated under isosmotic (300 mosmol/kg H2O; pH 7.4) and hyperosmotic (400, 500, or 600 mosmol/kg H2O) culture and assay conditions by the use of a specific and sensitive RIA. During isosmotic incubation, MDCK cells, which may be of collecting duct origin, secreted by
H, Schramek, G, Gstraunthaler, C C, Willinger, W, Pfaller   +3 more
openaire   +2 more sources

The IQGAP1‐Claudin4‐JNK Signaling Axis as a Differential Biomarker in Renal Cell Carcinoma

Clinical and Translational Science, Volume 19, Issue 4, April 2026.
ABSTRACT Renal cell carcinoma (RCC) is a highly heterogeneous malignant neoplasm with multiple morphological and molecular subtypes; however, the basis of this heterogeneity remains incompletely defined, hindering effective diagnosis and treatment. The signaling scaffold IQGAP1 and claudins are tight junction protein partners involved in epithelial ...
Nahshon Puente, Xiuzhen Fan, Nagalakshmi Nadiminty, Obi Ekwenna, Firas G. Petros, Puneet Sindhwani, Amira Gohara, Evgeny Yakirevich, Mahasin A. Osman   +8 more
wiley   +1 more source

Molecular characterization and antiviral effects of canine interferon regulatory factor 1 (CaIRF1)

BMC Veterinary Research, 2022
Background Interferon regulatory factor 1 (IRF1) is an important transcription factor that activates the type I interferon (IFN-I) response and plays a vital role in the antiviral immune response.
Xiangqi Hao, Hui Chen, Yanchao Li, Bo Chen, Weifeng Liang, Xiangyu Xiao, Pei Zhou, Shoujun Li   +7 more
doaj   +1 more source

Differential sensitivity of Madin-Darby canine kidney (MDCK) cells to epinephrine

The Journal of nutrition, health and aging, 2016
Catecholamines regulate a variety of cellular functions in the mammalian kidney. The present study was aimed to investigate the differential sensitivity of Madin-Darby Kidney Cells (MDCK cells) to epinephrine in a dose-dependent manner. The loss of adhesion and altered cell shape were observed in MDCK cells.
P, Muthuraman, P C, Nagajyothi, M, Chandrasekaran, G, Enkhtaivan, B, Venkitasamy, D H, Kim, M, Cho, J, Shim   +7 more
openaire   +2 more sources

TGN38 recycles basolaterally in polarized Madin-Darby canine kidney cells. [PDF]

Molecular Biology of the Cell, 1994
Sorting of newly synthesized plasma membrane proteins to the apical or basolateral surface domains of polarized cells is currently thought to take place within the trans-Golgi network (TGN). To explore the relationship between protein localization to the TGN and sorting to the plasma membrane in polarized epithelial cells, we have expressed constructs
Rajasekaran, A, Humphrey, J, Wagner, M, Miesenböck, G, Le Bivic, A, Bonifacino, J, Rodriguez-Boulan, E   +6 more
openaire   +2 more sources