5-Methyl-cytosine stabilizes DNA but hinders DNA hybridization revealed by magnetic tweezers and simulations. [PDF]
Nucleic Acids Res, 2022 5-Methyl-cytosine (5mC) is one of the most important DNA modifications and plays versatile biological roles. It is well known that 5mC stabilizes DNA duplexes.
Zhao XC +8 more
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Micromechanical Study of Hyperacetylated Nucleosomes Using Single Molecule Transverse Magnetic Tweezers. [PDF]
Int J Mol Sci, 2023 Nucleosomes are stable complexes of DNA and histone proteins that are essential for the proper functioning of the genome. These structures must be unwrapped and disassembled for processes such as gene expression, replication, and repair.
Gaire S +5 more
europepmc +4 more sources
Direct Measurement of the Stall Torque of the Flagellar Motor in Escherichia coli with Magnetic Tweezers [PDF]
mBio, 2022 The flagellar motor drives the rotation of flagellar filaments, propelling the swimming of flagellated bacteria. The maximum torque the motor generates, the stall torque, is a key characteristic of the motor function.
Bin Wang +3 more
doaj +3 more sources
Magnetic tweezers: development and use in single-molecule research
BioTechniques, 2022 The use of magnetic tweezers for single-molecule micromanipulation has evolved rapidly since its introduction approximately 30 years ago. Magnetic tweezers have provided important insights into the dynamic activity of DNA-processing enzymes, as well as ...
Santosh Gaire +3 more
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Measuring molecular forces inside living cells using magnetic tweezers. [PDF]
Biophys RevTo change shape, move, grow and divide, cells employ various motor and non-motor proteins that convert chemical energy into the generation of mechanical force.
Kongari A, Molodtsov M.
europepmc +4 more sources
Correlating fluorescence microscopy, optical and magnetic tweezers to study single chiral biopolymers such as DNA [PDF]
Nature CommunicationsBiopolymer topology is critical for determining interactions inside cell environments, exemplified by DNA where its response to mechanical perturbation is as important as biochemical properties to its cellular roles.
Jack W. Shepherd +9 more
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A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins [PDF]
Molecules, 2021 We report data from single molecule studies on the interaction between single DNA molecules and core histones using custom-designed horizontal magnetic tweezers.
Roberto Fabian +5 more
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A Horizontal Magnetic Tweezers and Its Use for Studying Single DNA Molecules [PDF]
Micromachines, 2018 We report the development of a magnetic tweezers that can be used to micromanipulate single DNA molecules by applying picoNewton (pN)-scale forces in the horizontal plane. The resulting force–extension data from our experiments show high-resolution
Roberto Fabian +4 more
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The origin of different bending stiffness between double-stranded RNA and DNA revealed by magnetic tweezers and simulations. [PDF]
Nucleic Acids ResThe subtle differences in the chemical structures of double-stranded (ds) RNA and DNA lead to significant variations in their biological roles and medical implications, largely due to their distinct biophysical properties, such as bending stiffness ...
Dong HL +5 more
europepmc +4 more sources
Multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision [PDF]
Nature Communications, 2020 Single molecule force measurements have shed light on dynamic biological events, but rare events escape notice owing to low throughput of the methods. Here, the authors combine an array of magnetic tweezers with lateral flow to increase throughput 100 ...
Rohit Agarwal, Karl E. Duderstadt
doaj +4 more sources