Stable isotope metabolomics of pulmonary artery smooth muscle and endothelial cells in pulmonary hypertension and with TGF-beta treatment. [PDF]
Altered metabolism in pulmonary artery smooth muscle cells (PASMCs) and endothelial cells (PAECs) contributes to the pathology of pulmonary hypertension (PH), but changes in substrate uptake and how substrates are utilized have not been fully ...
D' Alessandro, Angelo +12 more
core +1 more source
Analytical methods in wineries: is it time to change? [PDF]
A review of the methods for the most common parameters determined in wine—namely, ethanol, sulfur dioxide, reducing sugars, polyphenols, organic acids, total and volatile acidity, iron, soluble solids, pH, and color—reported in the last 10 years is ...
Andersen S. K. +51 more
core +1 more source
A Genetically Encoded Biosensor for Characterizing Transport and Metabolism of Glutarate
Here, a genetically encoded glutarate biosensor, Glusor, is developed based on transcriptional regulator CsiR. Glusor can quantify glutarate concentrations with good accuracy and precision. Then, the role of KgtP and YnfM are identified and characterized by using Glusor. Glusor also allows glutarate spatiotemporal resolution in live cells, facilitating
Kaiyu Gao +12 more
wiley +1 more source
The Interaction of Glutamate Dehydrogenase and Malate Dehydrogenase with Phospholipid Membranes [PDF]
The interaction between sonicated dispersions of purified phospholipids, corresponding to those found in the inner mitochondrial membrane, and the enzymes was studied by kinetic and spectroscopic methods.With glutamate dehydrogenase the charge type of the lipid head group determines the extent of lipid‐protein complexing.
openaire +5 more sources
On the pathways feeding the H₂ production process in nutrient-replete, hypoxic conditions : commentary on the article 'Low oxygen levels contribute to improve photohydrogen production in mixotrophic non-stressed Chlamydomonas cultures', by Jurado-Oller et al., Biotechnology for Biofuels, published September 7, 2015; 8:149 [PDF]
Background: Under low O-2 concentration ( hypoxia) and low light, Chlamydomonas cells can produce H-2 gas in nutrient-replete conditions. This process is hindered by the presence of O-2, which inactivates the [FeFe]-hydrogenase enzyme responsible for H-2
Scoma, Alberto, Toth, Szilvia Z
core +2 more sources
This study presents a refined adaptive laboratory evolution (ALE) strategy to resolve the trade‐off between tolerance and biosynthetic efficiency in toxic chemical production. Combining in vivo mutagenesis, microdroplet‐based evolution, and biosensor‐assisted selection, this strategy rapidly yields robust “win‐win” phenotypes.
Yufei Zhang +7 more
wiley +1 more source
This study engineers Yarrowia lipolytica to produce succinic acid (SA) from lignocellulosic biomass. Adaptive evolution and multi‐omics analysis identify regulators that alleviate xylose metabolic disorder. Multi‐copy integration of xylose metabolic genes resolves redox imbalances from a futile fatty acid cycle, producing high‐titer SA from corn stover
Yutao Zhong +7 more
wiley +1 more source
Sources of reducing equivalents for nitrite reductase in Pisum arvense roots
Glucose-6-phosphate and NADP+ as well as malic acid and NADP+ present in the incubation mixture enhanced nitrite reductase (EC 1.6.6.4) activity in Pisum arvense roots.
Grażyna Kłobus
doaj +1 more source
Two sibling species in the Pellia epiphylla (L.) Corda (n=9) complex, viz. Pellia epiphylla sp. N and Pellia epiphylla sp. S were compared in their protein concentration and in the dynamics and also in relative activity of some enzymes: malate ...
Wiesław Prus-Głowacki +2 more
doaj +1 more source
DYNAMICS OF BRAIN ENZYMES ACTIVITY IN RAT EXPOSED TO HYPOXIA [PDF]
The aim of the work was to study the dynamics of lactate dehydrogenase (LDH; EC 1.1.1.27), aconitase (AH; EC 4.2.1.3), NAD-dependent malate dehydrogenase (MDH; EC 1.1.1.37), succinate dehydrogenase (SDH; EC 1.3.99.1) activity in homogenates and sub ...
A. M. Rashidova +3 more
doaj +1 more source

