Results 131 to 140 of about 167,858 (299)

MALDI-TOF MS of trypsin-digested αSyn.

, 2018
MALDI-TOF mass spectra of trypsin-digested samples of αSyn purified from E. coli transformed with pET21a-alpha-synuclein alone (A) or pET21a-alpha-synuclein+pTSara-NatB (B) and only induced with 1 mM IPTG (see Table 1). The ~42 Da shift in the N-terminal
Dushyant Shailesh Patel (5504252), Matteo Rovere (5504249), Alex Edward Powers (5504255), Tim Bartels (606170)   +3 more
core   +1 more source

Nuclease‐Free Scalable Purification of Physalis Mottle Virus‐Like Particles Expressed in Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Plant virus‐like particles (VLPs) are promising nanotechnology platforms, but their translation requires purification strategies suitable for large‐scale production. Here, we developed a nuclease‐free downstream process for Physalis mottle virus (PhMV) VLPs expressed in Escherichia coli, combining ammonium sulfate precipitation, low‐pH ...
Sean K. Hsu, Patrick Opdensteinen, Tariq Hussain, Nicole F. Steinmetz   +3 more
wiley   +1 more source

Pre-trained Maldi Transformers improve MALDI-TOF MS-based prediction

Computers in Biology and Medicine
Abstract For the last decade, matrix-assisted laser desportion/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been the reference method for species identification in clinical microbiology. Hampered by a historical lack of open data, machine learning research towards models specifically adapted to MALDI-TOF MS remains in ...
Gaetan De Waele, Gerben Menschaert, Peter Vandamme, Willem Waegeman   +3 more
openaire   +2 more sources

Nanotopographical cues mechanically regulate the secretion and osteoinductive potential of MSC‐derived exosomes for bone regeneration

BMEMat, EarlyView.
A defined 700 nm low‐density nanotopographical interface mechanically primes MSCs through the integrin β1–focal adhesion–Akt–Rab27a‐dependent pathway, enhancing exosome secretion and osteoinductive potency. Enrichment of miR‐548d‐5p in these exosomes promotes osteogenic differentiation and bone regeneration, establishing nanotopography‐guided MSC ...
Ziqi Zhang, Limeng Zheng, Zehui Xu, Xiaoyang Han, Guangli Xiang, Yizeng Chang, Hong Chen, Mo Yang, Wai Yin Cheng, Nathanael Chun Him Lai, Weihao Yuan, Siu Hong Dexter Wong, Bohan Yin   +12 more
wiley   +1 more source

MALDI-MS Evaluation of Poly(3-hexylthiophene) Synthesized by Chemical Oxidation with FeCl₃

, 2016
Poly(3-hexylthiophene) synthesized by chemical oxidation with FeCl3 was investigated with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) using either dithranol or the electron-transfer matrices terthiophene and anthracene.
Robin L. McCarley (57433), C. J. DuBois, (3208617), Tracy Donovan McCarley (3208611), Noble (3208614)   +3 more
core   +1 more source

Small scale purification and biophysical characterization of haptoglobin derived from human Cohn fraction IV

Biotechnology Progress, EarlyView.
Abstract Haptoglobin (Hp) is a polymorphic acute phase α‐2 glycoprotein found in plasma that plays a critical role in binding, neutralizing, and removing cell‐free hemoglobin (Hb) from the circulation. Under clinical conditions characterized by high levels of hemolysis, such as in patients with sickle cell disease (SCD), large quantities of cell‐free ...
Shuwei Lu, Mohd Asim Khan, Pedro Cabrales, David C. Irwin, Paul W. Buehler, Andre F. Palmer   +5 more
wiley   +1 more source

Uncatalyzed Guanosine Phosphorylation under Potentially Prebiotic Conditions

Chemistry – A European Journal, EarlyView.
Pyrophosphate acts as phosphorylating agent of guanosine under simple wet‐dry cycles without condensing agent and inorganic catalysts to afford cyclic guanosine monophosphate derivatives useful for oligomerization in prebiotic processes. ABSTRACT The phosphorylation of nucleosides and their subsequent polymerization are key issues in the study of the ...
Martina Proietti, Bruno Mattia Bizzarri, Elisa De Marchi, Alessandra Giorgi, Raffaele Saladino, Giovanna Costanzo   +5 more
wiley   +1 more source

MALDI-TOF Mass Spectrometry Based on Parylene-Matrix Chip for the Analysis of Lysophosphatidylcholine in Sepsis Patient Sera

, 2019
In this work, medical diagnosis of sepsis was conducted via quantitative analysis of lysophosphatidylcholine 16:0 (LPC 16:0) by using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry based on a parylene-matrix chip.
Sohee Yoon (7573403), Nam Su Ku (7573400), Jae-Chul Pyun (1732594), Joo-Yoon Noh (6724649), Mi Hwa Shin (7573397), Eun Hye Lee (693410), Moon-Ju Kim (823996), Min-Jung Kang (12050), Tae Gyeong Yun (6724646), Kyung Soo Chung (2735668), Sang-Guk Lee (3696133), Jong-Min Park (3995360), Moo Suk Park (7504892)   +12 more
core   +1 more source

Bacterial Species Associated With Chronic Inflammation and Oral Cancer. A Comparative Study Between Patients With HNC and Patients With Chronic Periodontitis

Cancer Nexus, EarlyView.
ABSTRACT Objective To compare the presence and quantification of selected periodontopathogenic bacterial species and salivary parameters between patients with head and neck cancer (HNC) treated with radiotherapy and patients with chronic periodontitis, using chronic periodontitis as a reference model of oral dysbiosis.
Tanya Pereira‐Riveros, José López‐López, Alicia Lozano‐Borbalas, Julio Paciello‐Coronel, Teresa Vinuesa   +4 more
wiley   +1 more source

Impact of un-polymerized acrylamide monomer residues onto protein identification by MALDI TOF MS

Open Chemistry, 2012
Talian Ivan, Kováčová Veronika, Petrovič Marián, Sabo Ján   +3 more
doaj   +1 more source