Results 301 to 310 of about 940,374 (326)
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Analytical Chemistry, 2013
A periplasmic binding protein (PBP) was investigated as a novel binding species in a similar manner to an antibody in a competitive enzyme linked immunosorbent assay (ELISA), resulting in a highly sensitive and specific assay utilizing liposome-based ...
Katie A. Edwards, A. Baeumner
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A periplasmic binding protein (PBP) was investigated as a novel binding species in a similar manner to an antibody in a competitive enzyme linked immunosorbent assay (ELISA), resulting in a highly sensitive and specific assay utilizing liposome-based ...
Katie A. Edwards, A. Baeumner
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[75] Maltose-binding protein from Escherichia coli
1982Publisher Summary This chapter describes the assay method, the purification procedure, and the properties of maltose-binding protein from Escherichia coli . The maltose-binding protein is a periplasmic protein of Escherichia coli that is involved in the transport of maltose and maltodextrins across the bacterial envelope and in the chemotaxis ...
Odile K. Kellermann, Thomas Ferenci
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Kinetics and Energetics of the Translocation of Maltose Binding Protein Folding Mutants
Journal of Molecular Biology, 2008Protein translocation in Escherichia coli is mediated by the translocase that, in its minimal form, comprises a protein-conducting pore (SecYEG) and a motor protein (SecA). The SecYEG complex forms a narrow channel in the membrane that allows passage of secretory proteins (preproteins) in an unfolded state only.
Tomikiewicz, Danuta +3 more
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Export of the periplasmic maltose-binding protein ofEscherichia coli
Journal of Bioenergetics and Biomembranes, 1990The export of the maltose-binding protein (MBP), the malE gene product, to the periplasm of Escherichia coli cells has been extensively investigated. The isolation of strains synthesizing MalE-LacZ hybrid proteins led to a novel genetic selection for mutants that accumulate export-defective precursor MBP (preMBP) in the cytoplasm.
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Magnetic nanoparticles for the affinity adsorption of maltose binding protein (MBP) fusion enzymes
, 2012By the combination of fusion protein technology and nanoparticle surface modification technology, a maltose-modified magnetic support has been successfully developed for the integration of separation/purification, and immobilization of maltose binding ...
Lilin Zhou +7 more
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Dynamical persistence of active sites identified in maltose-binding protein
Journal of Molecular Modeling, 2017This study identifies dynamical properties of maltose-binding protein (MBP) useful in unveiling active site residues susceptible to ligand binding. The described methodology has been previously used in support of novel topological techniques of persistent homology and statistical inference in complex, multi-scale, high-dimensional data often ...
Dragan Nikolić +1 more
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Membrane Protein Topology Determination by Proteolysis of Maltose Binding Protein Fusions
Analytical Biochemistry, 1993A method is presented for determining the topology of Escherichia coli inner membrane proteins that is based on proteolysis of fusion proteins between maltose binding protein (MBP) and the membrane protein of interest. Fusion proteins are constructed wherein the MBP domain is fused upstream of the membrane protein domain.
J. Olson +3 more
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Expression and Purification of Recombinant Proteins by Fusion to Maltose-Binding Protein
Molecular Biotechnology, 2000The pMAL vectors provide a method for purifying proteins from cloned genes by fusing them to maltose-binding protein (MBP, product of malE), which binds to amylose. The vectors use the tac promoter and the translation initiation signals of MBP to give high-level expression of the fusion, and an affinity purification for MBP to isolate the fusion ...
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Induced fit of passenger proteins fused to Archaea maltose binding proteins
Biochemical and Biophysical Research Communications, 2006Maltose binding proteins (MBPs) are used as carriers for improving the solubility of passenger proteins. Our results indicate that the higher solubility of the fusions correlates with their elevated heat stability. Fusions of the otherwise thermo-sensitive GFP with MBPs from Archaea, but not GST-GFP and Escherichia coli MBP-GFP, maintained their ...
Ario de Marco +3 more
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Molecular Imprinting of Maltose Binding Protein: Tuning Protein Recognition at the Molecular Level
, 2011Protein imprinting in hydrogels is one approach for developing artificial receptors capable of specific recognition and binding of a target molecule. Through selection of monomers with side groups that can interact with the target protein and control ...
M. Zayats +3 more
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