Results 311 to 320 of about 940,374 (326)

Tetragonal Maltose Binding Protein

, 2021
A. Thaker, L. Sirajudeen, C. Simmons, B. Nannenga   +3 more
semanticscholar   +1 more source

A convenient method for affinity purification of maltose binding protein fusions

Journal of Biotechnology, 1998
Maltose binding protein of Escherichia coli is frequently employed as a fusion partner for the biosynthesis of polypeptides and proteins, largely because of the advantage provided by affinity purification of such a fusion. A mixture of cellulose and starch is shown to be a simple and effective alternative to the use of other, more complicated media ...
Usha Srinivasan, Jeffrey A. Bell
openaire   +2 more sources

Strategy for purifying maltose binding protein fusion proteins by affinity precipitation

Journal of Chromatography A, 2008
The maltose binding protein (MBP) affinity tag has been extensively used for protein purification. A commercial grade cationic starch could precipitate MBP or an MBP-tagged protein quantitatively by simultaneous addition of 10% (w/v) polyethylene glycol (PEG) and 50 mM calcium chloride.
Raghava, Smita, Aquil, Samina, Bhattacharyya, Sanchari, Varadarajan, Raghavan, Gupta, Munishwar N   +4 more
openaire   +3 more sources

Folding and aggregation of export-defective mutants of the maltose-binding protein

Research in Microbiology, 2002
We previously characterized a defective-folding variant of the periplasmic maltose-binding protein, MalE31. To examine the alternative folding pathways open to the MalE31 precursor, we have analyzed the cellular fates of this aggregation-prone protein carrying altered signal sequences.
Sabine Hunke, Jean-Michel Betton, Denis Phichith   +2 more
openaire   +3 more sources

Tritium NMR spectroscopy of ligand binding to maltose-binding protein

Biochemistry, 1991
Tritium-labeled alpha- and beta-maltodextrins have been used to study their complexes with maltose-binding protein (MBP), a 40-kDa bacterial protein. Five substrates, from maltose to maltohexaose, were labeled at their reducing ends and their binding studied.
Philip G. Williams, Kalle Gehring, David E. Wemmer, Jeffrey G. Pelton, Hiromi Morimoto   +4 more
openaire   +3 more sources

Associative properties of the Escherichiacoli galactose binding protein and maltose binding protein

Biochemical and Biophysical Research Communications, 1982
The ligand-binding characteristics of periplasmic galactose-binding protein and maltose-binding protein of Escherichia coli are analyzed. The saturation function was decreased upon increasing protein concentration and the monomer-dimer equilibrium was shifted towards the monomeric protein form upon an increase of the ligand concentration.
openaire   +5 more sources

Bacterial overexpression and purification of soluble recombinant human serum albumin using maltose-binding protein and protein disulphide isomerase.

Protein Expression and Purification, 2019
M. Nguyen, Y. Heo, Bich Hang Do, Sangki Baek, C. Kim, Y. Jang, Weontae Lee, H. Choe   +7 more
semanticscholar   +1 more source

Purification of Proteins Fused to Maltose-Binding Protein.

Methods in molecular biology, 2017
M. Lebendiker, T. Danieli
semanticscholar   +1 more source

Maltose-Binding Protein as a Solubility Enhancer

2003
Jeffrey D. Fox, David S. Waugh
openaire   +3 more sources

How maltose influences structural changes to bind to maltose‐binding protein: Results from umbrella sampling simulation

Proteins: Structure, Function, and Bioinformatics, 2013
N. M. Mascarenhas, J. Kästner
semanticscholar   +1 more source