Results 61 to 70 of about 940,374 (326)

The remarkable solubility-enhancing power of Escherichia coli maltose-binding protein.

Postepy biochemii, 2016
A common problem encountered during the production of recombinant proteins, particularly in bacteria, is their tendency to accumulate in an insoluble and inactive form (i.e., as inclusion bodies).
D. Waugh
semanticscholar   +1 more source

Substrate induction and glucose repression of maltose utilization by Streptomyces coelicolor A3(2) is controlled by malR, a member of the lacI-galR family of regulatory genes [PDF]

, 1997
malR of Strepomyces coelicolor A3(2) encodes a homologue of the Lacl/Galr family of repressor proteins, and is divergently transcribed from the malEFG gene cluster, which encodes components of an ATP-dependent transport system that is required for ...
Bibb, M.J., Postma, P.W., Van Wezel, G.P., White, J., Young, J.P.W.   +4 more
core   +2 more sources

A Cost-Effective Immobilization Method for MBP Fusion Proteins on Microtiter Plates Using a Gelatinized Starch–Agarose Mixture and Its Application for Convenient Protein–Protein Interaction Analysis

Methods and Protocols, 2023
The detection and quantification of protein–protein interactions (PPIs) is a crucial technique that often involves the use of recombinant proteins with fusion protein tags, such as maltose-binding protein (MBP) and glutathione-S-transferase (GST).
Yuri Emoto, Ryoya Katayama, Emi Hibino, Sho Ishihara, Natsuko Goda, Takeshi Tenno, Yoshihiro Kobashigawa, Hiroshi Morioka, Hidekazu Hiroaki   +8 more
doaj   +1 more source

Dendritic glycopolymers based on dendritic polyamine scaffolds: view on their synthetic approaches, characteristics and potential for biomedical applications [PDF]

, 2014
In this review we highlight the potential for biomedical applications of dendritic glycopolymers based on polyamine scaffolds. The complex interplay of the molecular characteristics of the dendritic architectures and their specific interactions with ...
Abbott, Agarwal, Agashe, Agashe, Agrawal, Agrawal, Albersheim, Albertazzi, Amir, Anajwala, Andre, Andre, Andre, Anna Janaszewska, Antoni, Antoni, Aoi, Appelhans, Appelhans, Arima, Arima, Arima, Arima, Arima, Baek, Baigude, Baigude, Baker, Bandaru, Barbara Klajnert-Maculewicz, Barner-Kowollik, Bettinger, Bhadra, Bhadra, Boas, Bogdan, Bogdan, Bojarova, Borges, Boye, Branco de Barros, Branderhorst, Branson, Brigitte Voit, Calderon, Carlmark, Carlmark, Chabre, Chabre, Chabre, Chen, Chen, Cheng, Cheng, Choudhury, Ciepluch, Ciolkowski, Ciolkowski, Cloninger, Conte, Cook, Cummins, Danishefsky, de Paz, Diebold, Dietmar Appelhans, Dolenc, Dominguez, Dondoni, Drzewinska, Duncan, Duncan, Dutta, Dutta, Dutta, Dutta, Ennen, Erbacher, Fajac, Filimon, Fischer, Franiak-Pietryga, Gabius, Gajbhiye, Gajbhiye, Gajbhiye, Gajbhiye, Gajbhiye, Garcia-Vallejo, Garg, Ghirardello, Gorelik, Gorityala, Gringas, Grobarova, Grosse, Grosse, Grosse, Gupta, Gupta, Gutsch, Hatano, Hauptmann, Hauptmann, He, Hu, Höbel, Iha, Ikeda, Imberty, Ina, Jain, Jain, Jain, Jain, Jain, Jain, Janaszewska, Janaszewska, Jararaman, Jararaman, Jayaraman, Jia, Joanna Lazniewska, Kade, Kaminskas, Kantchev, Kempe, Kensinger, Kensinger, Kesharwani, Kiessling, Kihara, Kihara, Kim, Klajnert, Klajnert, Klajnert, Klajnert, Klajnert, Klementieva, Klementieva, Kottari, Krist, Ku, Kuhlbeil, Kumar, Kunath, Kuo, Kuo, Kurmi, Köth, Laurino, Leclercq, Lee, Li, Lindhorst, Liu, Liu, Llinares, Lopez-Marin, Ma, Majoinen, Malik, Maly, Mangold, Marradi, Martos, Martínez, Matsushita, Matsushita, McCarthy, McCarthy, McCarthy, McCarthy, McNermy, Medina, Medina, Medina, Mignani, Mignani, Mignani, Mignani, Milowska, Misra, Mkandawire, Monsigny, Motoyama, Nahar, Narayan, Needham, Nepogodiev, Nicolas, Niederhafener, Niederhafener, Niederhafener, Ogiso, Oka, Oka, Ottaviani, Palcic, Paolino, Paolino, Papadopoulos, Park, Park, Park, Park, Patel, Pedziwiatr-Werbicka, Percec, Polikarpov, Richter, Robb, Ross, Roy, Roy, Roy, Roy, Rudd, Röckendorf, Sakamoto, Santoyo-Gonzáles, Santoyo-Gonzáles, Sapsford, Sashiwa, Satoh, Schengrund, Seah, Sethi, Shah, Sharma, Sharon, Shaunak, Shcharbin, Shiao, Sinha, Solassol, Stefani, Sun, Supattapone, Svenson, Svenson, Szewczyk, Szulc, Szulc, Tekade, ten Brummelhuis, Thünemann, Ting, Touaibia, Tripp, Tsutsumiuchi, Turnbull, Vacas Córdoba, Vepŕek, Wada, Wada, Walter, Wang, Wang, Wang, Warenda, Wasiak, Wijagkanalan, Wolfenden, Woller, Wood, Wu, Wu, Zanini, Zanini, Zanta, Zhang, Ziemba, Ziemba, Ziemba, Ziemba, Zinselmeyer   +278 more
core   +2 more sources

Macrophage‐derived MLKL in alcohol‐associated liver disease: Regulation of phagocytosis

Hepatology, EarlyView., 2022
EtOH causes leaky gut allowing bacteria and PAMPs into the liver, resulting in hepatic inflammation and injury. We demonstrate that LPS induces STAT1‐mediated expression and phosphorylation of MLKL in macrophages and identify a novel function that myeloid MLKL translocates to phagosomes and lysosomes and regulates phagocytosis, which contributes to the
Xiaoqin Wu, Xiude Fan, Megan R. McMullen, Tatsunori Miyata, Adam Kim, Vai Pathak, Jianguo Wu, Le Z. Day, Josiah E. Hardesty, Nicole Welch, Jaividhya Dasarathy, Daniela S. Allende, Arthur J. McCullough, Jon M. Jacobs, Daniel M. Rotroff, Srinivasan Dasarathy, Laura E. Nagy   +16 more
wiley   +1 more source

In silico evidence that protein unfolding is as a precursor of the protein aggregation [PDF]

ChemPhysChem 2020, 21, 377, 2019
We present a computational study on the folding and aggregation of proteins in aqueous environment, as function of its concentration. We show how the increase of the concentration of individual protein species can induce a partial unfolding of the native conformation without the occurrence of aggregates. A further increment of the protein concentration
arxiv   +1 more source

Crystallization of the oligopeptide-binding protein AppA from Bacillus subtilis [PDF]

, 2004
AppA is the membrane-anchored extracellular receptor component of an ABC transporter responsible for the uptake of oligopeptides into Bacillus subtilis.
Blagova, E, Brannigan, J A, Chambers, J, Levdikov, V M, Pattenden, R J, Wilkinson, A J, Wright, L   +6 more
core   +1 more source

The malQ gene is essential for starch metabolism in Streptococcus mutans

Journal of Oral Microbiology, 2013
Background: The malQ and glgP genes, respectively, annotated as putative 4-α-glucanotransferase and putative glycogen phosphorylase are located with a 29 nucleotide overlap on the Streptococcus mutans genome.
Yutaka Sato, Kazuko Okamoto-Shibayama, Toshifumi Azuma   +2 more
doaj   +1 more source

A novel methodology on distributed representations of proteins using their interacting ligands [PDF]

Bioinformatics 2018, 2018
The effective representation of proteins is a crucial task that directly affects the performance of many bioinformatics problems. Related proteins usually bind to similar ligands. Chemical characteristics of ligands are known to capture the functional and mechanistic properties of proteins suggesting that a ligand based approach can be utilized in ...
arxiv   +1 more source

UV difference spectroscopy of ligand binding to maltose‐binding protein [PDF]

FEBS Letters, 1992
We have used UV absorbance spectroscopy to study the binding of linear and circular maltodextrins to maltose‐binding protein (MBP). Titrations with maltose yield three ??? points in the difference spectrum of MBP, consistent with two protein conformations: ligand‐free and ligand‐bound.
Kalle Gehring, Kogan Bao, Hiroshi Nikaido   +2 more
openaire   +3 more sources