Purifying natively folded proteins from inclusion bodies using sarkosyl, Triton X-100, and CHAPS
BioTechniques, 2010 We describe a rapid, simple, and efficient method for recovering glutathione S-transferase (GST)- and His6-tagged maltose binding protein (MBP) fusion proteins from inclusion bodies.
Hu Tao +5 more
doaj +1 more source
Giardia Cyst Wall Protein 1 Is a Lectin That Binds to Curled Fibrils of the GalNAc Homopolymer [PDF]
, 2010 The infectious and diagnostic stage of Giardia lamblia (also known as G. intestinalis or G. duodenalis) is the cyst. The Giardia cyst wall contains fibrils of a unique β-1,3-linked N-acetylgalactosamine (GalNAc) homopolymer and at least three cyst wall ...
Bullitt, Esther +6 more
core +5 more sources
Stubborn contaminants: influence of detergents on the purity of the multidrug ABC transporter BmrA. [PDF]
PLoS ONE, 2014 Despite the growing interest in membrane proteins, their crystallization remains a major challenge. In the course of a crystallographic study on the multidrug ATP-binding cassette transporter BmrA, mass spectral analyses on samples purified with six ...
Benjamin Wiseman +6 more
doaj +1 more source
Development of an electrochemical maltose biosensor [PDF]
, 2012 In this work, electrochemical maltose biosensors based on mutants of the maltose binding protein (MBP) are developed. A ruthenium II complex (Ru II ), which is covalently attached to MBP, serves as an electrochemical reporter of MBP conformational ...
García García, Alfonso +3 more
core +1 more source
The crystal structure of Pneumolysin at 2.0 Å resolution reveals the molecular packing of the pre-pore complex [PDF]
, 2015 Pneumolysin is a cholesterol-dependent cytolysin (CDC) and virulence factor of Streptococcus pneumoniae. It kills cells by forming pores assembled from oligomeric rings in cholesterol-containing membranes.
Andrew, Peter W +8 more
core +1 more source
Frontiers in Bioengineering and Biotechnology, 2020 Steroid hormones that serve as vital compounds are necessary for the development and metabolism of a variety of organisms. The neverland (NVD) family genes encode the conserved Rieske-type oxygenases, which are accountable for the dehydrogenation during ...
Shuhong Mao +14 more
doaj +1 more source
Maltose-binding protein is a potential carrier for oral immunizations [PDF]
, 2011 In humans and most animal species such as pigs, vaccination via the oral route is a prerequisite for induction of a protective immunity against enteropathogens. Hereto, live attenuated microorganisms can be used.
Bellot, Philippe +3 more
core +1 more source
Protein-mediated miRNA detection and siRNA enrichment using p19
BioTechniques, 2010 p19 RNA binding protein from the Carnation Italian ringspot virus (CIRV) is an RNA-silencing suppressor that binds small interfering RNA (siRNA) with high affinity.
Jingmin Jin +3 more
doaj +1 more source
The dual nature of trehalose in citrus canker disease: A virulence factor for Xanthomonas citri subsp. citri and a trigger for plant defence responses [PDF]
, 2015 Xanthomonas citri subsp. citri (Xcc) is a bacterial pathogen that causes citrus canker in susceptible Citrus spp. The Xcc genome contains genes encoding enzymes from three separate pathways of trehalose biosynthesis.
Feil, Regina +10 more
core +1 more source
Solution NMR studies of periplasmic binding proteins and their interaction partners
Biomolecular Concepts, 2011 Periplasmic binding proteins (PBPs) are a crucial part of ATP-binding cassette import systems in Gram-negative bacteria. Central to their function is the ability to undergo a large-scale conformational rearrangement from open-unliganded to closed ...
Pistolesi Sara +2 more
doaj +1 more source