Results 71 to 80 of about 74,095 (228)
Microbial Cell Factories, 2019 Background The production of therapeutically active single chain variable fragment (scFv) antibody is still challenging in E. coli due to the aggregation propensity of recombinant protein into inclusion bodies (IBs).
Animesh Sarker +2 more
doaj +1 more source
Crystal structure of human NLRP12 PYD domain and implication in homotypic interaction. [PDF]
PLoS ONE, 2018 NLRP12 is a NOD-like receptor that plays multiple roles in both inflammation and tumorigenesis. Despite the importance, little is known about its mechanism of action at the molecular level.
Tengchuan Jin +4 more
doaj +1 more source
BioTechniques, 2022 Antibody (Ab) validation is the procedure in which an Ab is thoroughly assayed for sensitivity and specificity in a given application. Validation of Abs against post-translationally modified (PTM) targets is particularly challenging because it requires ...
Kezzia S Jones +10 more
doaj +1 more source
Structural basis for the mechanism of ABC transporters. [PDF]
, 2015 The ATP-binding cassette (ABC) transporters are primary transporters that couple the energy stored in adenosine triphosphate (ATP) to the movement of molecules across the membrane.
Beis, K
core +1 more source
Interaction of two photoreceptors in the regulation of bacterial photosynthesis genes [PDF]
, 2012 The expression of photosynthesis genes in the facultatively photosynthetic bacterium Rhodobacter sphaeroides is controlled by the oxygen tension and by light quantity. Two photoreceptor proteins, AppA and CryB, have been identified in the past, which are
Frühwirth, Sebastian +5 more
core +1 more source
BioTechniques, 2010 We describe the use of an auto-induction medium containing N-(phosphono-methyl)glycine (glyphosate) as a means for high-level introduction of nonstandard aromatic amino acids into a protein.
Muniasamy Neerathilingam +1 more
doaj +1 more source
Prokaryotic soluble overexpression and purification of bioactive human growth hormone by fusion to thioredoxin, maltose binding protein, and protein disulfide isomerase. [PDF]
PLoS ONE, 2014 Human growth hormone (hGH) is synthesized by somatotroph cells of the anterior pituitary gland and induces cell proliferation and growth. This protein has been approved for the treatment of various conditions, including hGH deficiency, chronic renal ...
Minh Tan Nguyen +8 more
doaj +1 more source
Structure and function of negative feedback loops at the interface of genetic and metabolic networks [PDF]
, 2006 The molecular network in an organism consists of transcription/translation regulation, protein-protein interactions/modifications and a metabolic network, together forming a system that allows the cell to respond sensibly to the multiple signal molecules
Andersson, Anna M. C. +3 more
core +4 more sources
PLoS ONE, 2014 Human granulocyte colony-stimulating factor (hGCSF), a neutrophil-promoting cytokine, is an effective therapeutic agent for neutropenia patients who have undergone several cancer treatments. Efficient production of hGCSF using E.
Bich Hang Do +4 more
doaj +1 more source
A point mutation of integrin beta 1 subunit blocks binding of alpha 5 beta 1 to fibronectin and invasin but not recruitment to adhesion plaques. [PDF]
, 1992 A point mutation in a highly conserved region of the beta 1 subunit, Asp130 to Ala (D130A) substitution, abrogates the Arg-Gly-Asp (RGD)-dependent binding of alpha 5 beta 1 to fibronectin (FN) without disrupting gross structure or heterodimer assembly ...
Ginsberg, MH +4 more
core +2 more sources