Results 231 to 240 of about 14,391 (251)
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Kinetics of β-mannanase fermentation by Bacillus licheniformis
Biotechnology Letters, 2003Bacterial growth, konjac powder utilization and beta-mannanase production by Bacillus licheniformis NK-27 in batch fermentation were used to develop a model of the process. The optimal set of parameters was estimated by fitting the model to experimental data.
Feng, Y.Y. +6 more
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A simple assay procedure for β-d-mannanase
Carbohydrate Research, 1978A simple assay procedure for beta-D-mannanase enzyme has been developed which employs carob D-galacto-D-mannan dyed with Remazolbrilliant Blue. Additionally, the procedure is quantitative, relatively sensitive, and highly specific for beta-D-mannanase enzyme.
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Production, properties, and applications of endo-β-mannanases
Biotechnology Advances, 2017The present review provides up-to-date information on the occurrence and methodologies used for producing and purifying endo-β-mannanases and a comprehensive comparison of their biochemical properties. The amalgamation of biochemical, molecular and structural biology approaches which have been used for understanding endo-β-mannanase families, catalytic
Praveen Kumar, Srivastava +1 more
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Purification and some properties of ?-mannanase fromPolyporus versicolor
World Journal of Microbiology & Biotechnology, 1990Multiple enzyme forms of β-mannanase activity fromPolyporus versicolor were puritied to molecular homogeneity by a sequence involving DEAE Bio-Gel A chromatography, gel filtration on Sephadex G-100 and high-performance liquid chromatography using anion exchange and hydrophobic Interaction media.
Johnson, K., Ross, Neil, Schneider, H.
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Purification and properties of mannanase from Bacillus subtilis
World Journal of Microbiology & Biotechnology, 1994Extracellular mannanase from Bacillus subtilis NM-39, an isolate from Philippine soil, was purified about 240-fold with a yield of 7.3% by ammonium sulphate fractionation, DEAE-Toyopearl chromatography and Sephacryl S-200 gel filtration. Its M r was 38 kDa and it had a pI of 4.8 and optimum activity at pH 5.0 and 55°C.
N S, Mendoza +4 more
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Enzyme and Microbial Technology, 2016
Gene encoding for β-mannanase (E.C 3.2.1.78) from Klebsiella oxytoca KUB-CW2-3 was cloned and expressed by an E. coli system resulting in 400 times higher mannanase activities than the wild type. A 3314bp DNA fragment obtained revealed an open reading frame of 1164bp, namely kman-2, which encoded for 387 amino acids with an estimated molecular weight ...
Nawapan, Pongsapipatana +5 more
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Gene encoding for β-mannanase (E.C 3.2.1.78) from Klebsiella oxytoca KUB-CW2-3 was cloned and expressed by an E. coli system resulting in 400 times higher mannanase activities than the wild type. A 3314bp DNA fragment obtained revealed an open reading frame of 1164bp, namely kman-2, which encoded for 387 amino acids with an estimated molecular weight ...
Nawapan, Pongsapipatana +5 more
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Archives of Microbiology, 1989
The gene encoding β-mannanase was cloned from alkalophilic Bacillus sp. AM-001 into Escherichia coli JM 101 by inserting HindIII-generated DNA fragments into the HindIII site of pUC19. A 2.0 kb XbaI-PstI fragment of the donor strain DNA was sufficient for β-mannanase synthesis. The amount of β-mannanase expressed in E.
Toshiro Akino +2 more
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The gene encoding β-mannanase was cloned from alkalophilic Bacillus sp. AM-001 into Escherichia coli JM 101 by inserting HindIII-generated DNA fragments into the HindIII site of pUC19. A 2.0 kb XbaI-PstI fragment of the donor strain DNA was sufficient for β-mannanase synthesis. The amount of β-mannanase expressed in E.
Toshiro Akino +2 more
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Functional enrichment of mannanase-treated spent brewer yeast
Preparative Biochemistry & Biotechnology, 2017In this study, the effect of Bacillus amyloliquefaciens-produced β-mannanase on the nutrient diffusion (release) and antioxidant activity of spent brewer yeast (SBY) was investigated. Three pretreatments were performed: (1) autolysis at 50°C for 24 h; (2) autolysis at 50°C for 24 h, with the addition of β-mannanase during the autolysis; (3) autolysis ...
Cao, R +5 more
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ß-Mannan and mannanase in poultry nutrition
World's Poultry Science Journal, 2015Dietary plant s-mannans are one of the major anti-nutritional components in monogastric nutrition. The presence of s-mannans in poultry diets has been associated with many negative features ranging from high intestinal viscosity and low nutrient digestibility to adverse effects on innate immune response and microbial proliferation in the gut.
Y. Shastak +4 more
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