A Method to Study Complex Enzyme Kinetics Involving Numerical Analysis of Enzymatic Schemes. The Mannitol Permease of Escherichia coli as an Example [PDF]
An analysis of complex kinetic mechanisms is proposed that consists of two steps, (i) building of an kinetic scheme from experimental data other than steady-state kinetics and (ii) numerical simulation and analysis of the kinetics of the proposed scheme ...
Lolkema, Juke S.,
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A novel compartment, the 'subqpical stem' of the aerial hyphae, is the location of a sigN-dependent, developmentally distinct transcription in Streptomyces coelicolor. [PDF]
Streptomyces coelicolor has nine SigB-like RNA polymerase sigma factors, several of them implicated in morphological differentiation and/or responses to different stresses. One of the nine, SigN, is the focus of this article.
Bignell D.R. +21 more
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Structural and Functional Characterization of EXPO‐Derived Extracellular Vesicles in Plants
In this study, 3D electron tomography (ET), cryo‐ET, and immunogold transmission electron microscopy (TEM) are employed to characterize plant extracellular vesicles (EVs) under physiological conditions. EVs are classified into three distinct categories according to their size, content, and molecular‐marker profiles. Furthermore, Exo70E2‐positive medium
Jiayang Gao +12 more
wiley +1 more source
The data on the mannitol content in different parts of the brown algae F. vesiculosus thallus from the Barents Sea have been obtained for the first time in different seasons.
Klindukh M. P.
doaj +1 more source
Intestinal barrier tightening by a cell-penetrating antibody to Bin1, a candidate target for immunotherapy of ulcerative colitis. [PDF]
Patients afflicted with ulcerative colitis (UC) are at increased risk of colorectal cancer. While its causes are not fully understood, UC is associated with defects in colonic epithelial barriers that sustain inflammation of the colon mucosa caused by ...
Alexander, Walker +8 more
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Seasonal cold adaptation is vital for insect survival, yet the molecular mechanisms linking diapause to mitochondrial resilience remain largely unresolved. We identify ascaroside C9 (asc‐C9) as a key endocrine signal that enhances diapause survival during cold stress by activating the AKHR–PGC1α–UCP4 axis, thereby driving cold‐induced lipolysis and ...
Jiao Zhou +14 more
wiley +1 more source
Cell volume regulation in the proximal tubule of rat kidney proximal tubule cell volume regulation [PDF]
We developed a dynamic model of a rat proximal convoluted tubule cell in order to investigate cell volume regulation mechanisms in this nephron segment.
Edwards, Aurelie, Layton, Anita T.
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Depression, a prevalent neuropsychiatric disorder with unclear pathogenesis, involves dysfunctional adenylyl cyclase 8 (Adcy8) as a key risk factor. Chronic stress selectively reduces Adcy8 expression in the dorsal CA1 (dCA1) neurons. Depletion of Adcy8 in dCA1 excitatory neurons induces depressive‐like behaviors by impairing neuronal excitability and ...
Zi‐Jie Liu +14 more
wiley +1 more source
The presence of the fermentable sugar d-mannitol in the diet improves nitrogen (N) utilization in rabbits. To clarify the mechanism by which d-mannitol improves N utilization, we studied the effect of d-mannitol on the fate of blood urea N in growing ...
L. Xiao +5 more
doaj +1 more source
Conjugated linoleic acid reduces permeability and fluidity of adipose plasma membranes from obese Zucker rats [PDF]
NOTICE: this is the author’s version of a work that was accepted for publication in Biochemical and Biophysical Research Communications. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and ...
Lopes, Paula A. +7 more
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