Crystal Structure of the Mannose-6-Phosphate Uncovering Enzyme [PDF]
Structure, 2020 Most lysosomal hydrolytic enzymes reach their destination via the mannose-6-phosphate (M6P) pathway. The enzyme N-acetylglucosamine-1-phosphodiester α-N-acetylglucosaminidase (NAGPA, or "uncovering enzyme") catalyzes the second step in the M6P tag formation, namely the removal of the masking N-acetylglucosamine (GlcNAc) portion. Defects in this protein
A. Gorelik, Katalin Illes, Bhushan Nagar
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Expression of Tailored α-N-Acetylglucosaminidase in Escherichia coli for Synthesizing Mannose-6-Phosphate on N-Linked Oligosaccharides of Lysosomal Enzymes [PDF]
BioengineeringLysosomal enzymes are synthesized as N-glycosylated glycoproteins with mannose-6-phosphate (M6P) moieties, which are responsible for their binding to M6P receptors and transporting to the lysosome. In the M6P biosynthetic pathway, a Man8GlcNAc2 glycoform
Yunsong Cao, Wei Wang
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Targeted protein degradation through site-specific antibody conjugation with mannose 6-phosphate glycan [PDF]
mAbsRecent developments in targeted protein degradation have provided great opportunities to eliminating extracellular protein targets using potential therapies with unique mechanisms of action and pharmacology.
Kaori Mukai +11 more
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Elevated mRNA expression and defective processing of cathepsin D in HeLa cells lacking the mannose 6‐phosphate pathway [PDF]
FEBS Open Bio, 2021 Disruption of the mannose 6‐phosphate (M‐6‐P) pathway in HeLa cells by inactivation of the GNPTAB gene, which encodes the α/β subunits of GlcNAc‐1‐phosphotransferase, results in missorting of newly synthesized lysosomal acid hydrolases to the cell ...
Lin Liu, Balraj Doray
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Identification of a fourth mannose 6-phosphate binding site in the cation-independent mannose 6-phosphate receptor. [PDF]
Glycobiology, 2015 The 300 kDa cation-independent mannose 6-phosphate receptor (CI-MPR) plays an essential role in lysosome biogenesis by targeting ∼ 60 different phosphomannosyl-containing acid hydrolases to the lysosome. This type I membrane glycoprotein has a large extracellular region comprised of 15 homologous domains.
Olson LJ +6 more
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Engineered therapeutic antibodies with mannose 6-phosphate analogues as a tool to degrade extracellular proteins [PDF]
Frontiers in ImmunologyInducing the degradation of pathological soluble antigens could be the key to greatly enhancing the efficacy of therapeutic monoclonal antibodies (mAbs), extensively used in the treatment of autoimmune and inflammatory disorders or cancer.
Morgane Daurat +13 more
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Lysosomal enzyme binding to the cation-independent mannose 6-phosphate receptor is regulated allosterically by insulin-like growth factor 2 [PDF]
Scientific ReportsThe cation-independent mannose 6-phosphate receptor (CI-MPR) is clinically significant in the treatment of patients with lysosomal storage diseases because it functions in the biogenesis of lysosomes by transporting mannose 6-phosphate (M6P)-containing ...
Richard N. Bohnsack +8 more
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Indian Journal of Ophthalmology, 2018 Purpose: To investigate the presence and patterns of lysosomal enzymes and mannose 6-phosophate receptor (MPRs) in human lacrimal drainage system. Methods: The study was performed on healthy lacrimal sacs and nasolacrimal ducts obtained from exenteration
Mohammad Javed Ali +3 more
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Cation-Independent Mannose 6-Phosphate Receptor Deficiency Enhances β-Cell Susceptibility to Palmitate. [PDF]
Mol Cell Biol, 2018 Baldwin AC +7 more
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Mannose-6-phosphate receptor: a novel regulator of T cell immunity. [PDF]
Cell Mol Immunol, 2018 Ara A, Ahmed KA, Xiang J.
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