Results 91 to 100 of about 2,775,388 (305)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

基于并联机器人的柔性装配工装构型精度综合

open access: yesJixie chuandong, 2014
A flexible fixture configuration for Aero-engine digital assembly is designed.Then aiming at the geometric feature of 3-UPU parallel mechanism,the error model is built considering universal hinge and rod length.Monte Carlo’s methods are adopted to ...
石宏, 朱宁, 李伟楠, 李楠
doaj  

An Inverse Analysis Method of Transmission Accuracy of Harmonic Reducer based on Multi-factor Coupling

open access: yesJixie chuandong, 2021
Transmission error of harmonic reducer is coupled by eccentric vectors such as geometric eccentricity and motion eccentricity during parts machining and assembly, and has frequency domain characteristics.
Linna Zhao   +9 more
doaj  

Jeffrey Manufacturing Company Shop View

open access: yes, 1890
Exterior view of the Jeffrey Manufacturing Company factories, Columbus, Ohio, ca. 1890. The particular use of each building is unknown.
The Jeffrey Manufacturing Company (Photographer)
core  

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Research of Digital Closed-loop Manufacturing Technology of Curvic Coupling

open access: yesJixie chuandong, 2020
In order to solve the problem that the coloring style of curvic coupling is not up to standard due to tooth profile error, it is difficult to quickly identify the root cause of the problem and it is difficult to adjust the process parameters, a digital ...
Zhiyong Wang   +3 more
doaj  

Jeffrey Manufacturing Company mine ventilation fan and employee photograph

open access: yes, 1912
A photograph of an employee of the Jeffrey Manufacturing Company in Columbus, Ohio, posing beside a mine ventilation fan manufactured by the company. The Jeffrey Manufacturing Company, also known as the Jeffrey Mining Corporate Center, was established ...
Jeffrey Manufacturing Company
core  

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

open access: yesFEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta   +7 more
wiley   +1 more source

Using cell‐free RNA to identify B‐ and T‐cell clonality for diagnosis and monitoring of B‐ and T‐cell neoplasms

open access: yesFEBS Open Bio, EarlyView.
Using peripheral blood for determining B‐cell or T‐cell clonality is more reliable when we use cell‐free RNA (cfRNA) because cells release blood significantly more RNA than DNA. Next‐generation sequencing (NGS) of cfRNA allows us to evaluate fragment cfRNA and evaluate clonality reliably without the need for prior determination of the specific dominant
Adam Albitar   +11 more
wiley   +1 more source

Editors’ Choice—Review—Sensor-Based and Computational Methods for Error Detection and Correction in 3D Printing

open access: yesECS Sensors Plus
Additive manufacturing in the realm of 3D printing has transformed the manufacturing sector, providing unmatched freedom in design and rapid prototyping abilities.
Prateek Mehta   +5 more
doaj   +1 more source

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