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Mass Cytometry of Hematopoietic Cells
2020Mass cytometry is now a well-established method that enables the measurement of 40-50 markers (generally proteins but transcripts are also possible) in single cells. Analytes are detected via antibodies tagged with heavy metal and detected by using a time-of-flight mass spectrometer.
Jager A., Sarno J., Davis K. L.
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Aptamer-facilitated mass cytometry
Analytical and Bioanalytical Chemistry, 2018Mass cytometry is a novel cell-by-cell analysis technique, which uses elemental tags instead of fluorophores. Sample cells undergo rapid ionization in inductively coupled plasma and the ionized elemental tags are then analyzed by means of time-of-flight mass spectrometry. Benefits of the mass cytometry approach are in no need for compensation, the high
Gleb G, Mironov +5 more
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Mass Cytometry in Food Allergy Research
2023Mechanisms underlying food allergy are not well understood. Mass cytometry is a technique that allows the multiple analysis of cell surface markers and intracellular proteins by using the spectrum of rare metal isotopes of different atomic masses without channel overlap.
Nathalie, Acevedo +1 more
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Analysis of Mass Cytometry Data
2019The CyTOF system produces single cell protein expression data similar to that from flow cytometry, but with an increased number of features measured. Traditionally, analysis of these data is carried out using manual gating, but with the increased dimensionality, manual gating becomes a suboptimal analysis strategy in some cases.
Christina B, Pedersen, Lars R, Olsen
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Signal Amplification for Imaging Mass Cytometry
Bioconjugate Chemistry, 2019An enzyme-catalyzed reporter deposition stain has been developed for Imaging Mass Cytometry (IMC). The reagent consists of an alkaline phosphatase substrate tethered to a tellurophene which serves as reporter group for mass cytometry. Upon phosphate hydrolysis, a quinone methide is released which covalently labels local nucleophiles. This strategy is a
Rahul Rana +3 more
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Kupffer Cell Characterization by Mass Cytometry
2020Kupffer cells are the liver-resident macrophages lining the sinusoids and are mostly known for their role of scavengers, as crucial keepers of organ integrity. But due to the many fundamental functions of the liver notably linked to detoxication, metabolism, protein synthesis, or immunology, Kupffer cells are exposed to a dynamic environment and ...
Camille, Blériot +4 more
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Titration of Mass Cytometry Reagents
2019Mass cytometry (MC) is a powerful research tool enabling high-dimensional analysis of single cells in suspension and within tissue sections following laser ablation. Here we describe the procedure of titrating metal-conjugated antibodies, to ensure that optimal levels of staining are achieved while minimizing nonspecific signals that may occur at high ...
Caryn, van Vreden +3 more
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Immunophenotyping by Mass Cytometry
2019Mass cytometry is a novel technology similar to flow cytometry in which antibodies are tagged with heavy metal molecules rather than fluorophores and then detected with time-of-flight mass spectrometry. This enables measurement of up to 50 simultaneous parameters with no autofluorescent background and little or no spillover or required compensation ...
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Mass cytometry: blessed with the curse of dimensionality
Nature Immunology, 2016Immunologists are being compelled to develop new high-dimensional perspectives of cellular heterogeneity and to determine which applications best exploit the power of mass cytometry and associated multiplex approaches.
Evan W Newell, Yang Cheng
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Imaging Mass Cytometry in Immuno-Oncology
2023In situ profiling of the tumor-immune microenvironment (TiME) requires the ability to co-localize and detect multiple proteins simultaneously. Imaging mass cytometry (IMC), using the Hyperion™ imaging system is a novel multiplex imaging modality that currently enables detection of up to 50 markers on fixed tissues at subcellular resolution and thus has
Tiziana, Cotechini +2 more
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