Results 81 to 90 of about 1,123,387 (315)

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Imaging mass spectrometry of polymeric materials

, 2009
Imaging mass spectrometry (MS) is a technique that makes images of molecular distributions at surfaces based on mass spectral information. At a range (typically a raster) of positions, mass spectra are measured from the surface giving a characteristic ...
Klerk, L.A., Massaspectrometrie, Sub Biomol.Mass Spectrometry & Proteom., Biomolecular Mass Spectrometry and Proteomics   +3 more
core  

High-mass-resolution MALDI mass spectrometry imaging of metabolites from formalin-fixed paraffin-embedded tissue.

, 2016
Formalin-fixed and paraffin-embedded (FFPE) tissue specimens are the gold standard for histological examination, and they provide valuable molecular information in tissue-based research.
McDonnell, L.A., Kuppen, P.J.K., Ly, A., Velde, C.J.H. van de, Kuppen, P.J., Kuppen, Peter J K, Buck, Achim, van de Velde, Cornelis J. H., Walch, A.K., Langer, R., Feuchtinger, A., Walch, A., Sun, Na, Janssen, Klaus-Peter, Balluff, Benjamin, Gorzolka, Karin, Weirich, Gregor, Langer, Rupert, Erlmeier, Franziska, Kuppen, Peter J. K., Aubele, M., Weirich, G., McDonnell, Liam, Walch, Axel, Zitzelsberger, Horst, Erlmeier, F., Ly, Alice, Sun, N., van de Velde, Cornelis J H, Aichler, Michaela, Buck, A., van de Velde, C.J., Feuchtinger, Annette, Zitzelsberger, H., Balluff, B., Aichler, M., McDonnell, L., Janssen, K.P., Aubele, Michaela, Gorzolka, K.   +39 more
core   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

A Facile Synthesis of ZnO Nanorods on Nitrogen-Doped Graphene Sheets for Supercapacitor Applications

International Journal of Electrochemical Science, 2020
ZnO nanorods/nitrogen-doped graphene (ZnO@NG) composite networks were prepared by a convenient hydrothermal method from zinc acetate and graphene oxide (GO) using ethylene glycol as the reducing agent.
Jianping Xu, Jie Huang, Meiwu Zhu, Chuanhong Cheng, Tianxiang Jin, Bin Huang   +5 more
doaj   +1 more source

Proteomic tools to study drug function [PDF]

, 2018
With the ever growing repertoire of drugs being developed, new unbiased methods are urgently needed that allow fast screening of protein targets and off-targets.
Leijten, Niels, Mulder, Celine, Lemeer, Simone, Sub Biomol.Mass Spectrometry & Proteom., Biomolecular Mass Spectrometry and Proteomics   +4 more
core   +1 more source

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

Automatic Generic Registration of Mass Spectrometry Imaging Data to Histology Using Nonlinear Stochastic Embedding

, 2014
The combination of mass spectrometry imaging and histology has proven a powerful approach for obtaining molecular signatures from specific cells/tissues of interest, whether to identify biomolecular changes associated with specific histopathological ...
Dijkstra, J., McDonnell, L.A., Heeren, Ron M. A., Abdelmoula, W.M., Dijkstra, Jouke, Maaten, L. van der, Heeren, R.M.A., Lelieveldt, Boudewijn P. F., Abdelmoula, Walid M., Morreau, Hans, Lelieveldt, B.P.F., Skraskova, Karolina, Tolner, Else A., Skraskova, K., Morreau, H., van der Maaten, Laurens, Carreira, R.J., Balluff, B., Tolner, E.A., Maagdenberg, A.M.J.M. van den, van den Maagdenberg, Arn M. J. M., Balluff, Benjamin, McDonnell, Liam A., Carreira, Ricardo J.   +23 more
core   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Mass Spectrometry and Tandem Mass Spectrometry: An Overview

, 2014
In this chapter, a brief overview of fundamentals in mass spectrometry and of its applications in homeland security, forensic sciences and explosive detection, are presented.
openaire   +3 more sources